Abstract
The ribonucleic acids extracted from nongerminated teliospores of Ustilago maydis showed the spectrum similar to histone-DNA complex with a high 230/260 mμ ratio. The chemical analyses of the RNA preparations revealed that the spectral abnormality was due to the complexing of RNA with a protein. On the other hand RNA prepared from the isolated ribosomes of spores exhibited a normal spectrum and low protein content relative to RNA fraction. These observations show that an unusual protein is present in germinated spores which complexes with RNA and disappears as germination occurs.
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Caltrider, P. G. and Gottlieb, D. 1966. Effects of sugars on germination and metabolism of teliospores of Ustilago maydis.—Phytopathology 56: 479–484.
Dunkle, L. D., Maheshwari, R. and Allen, P. J. 1969. Infection structures from rust urediospores: Effect of RNA protein synthesis inhibitors.—Science 163: 481–482.
Eisenstadt, J. M. and Brawerman, G. 1967. Purification of ribosomes from Euglena gracilis, p. 494–498. In L. Grossman and K. Moldave (eds.), Methods in Enzymology, Vol. XII, Part A.—Academic Press, New York.
Gottlieb, D., Rao, M. V. and Shaw, P. D. 1968. Changes in ribonucleic acid during the germination of teliospores of Ustilago maydis.—Phytopathology 58: 1593–1597.
Gierer, A. and Schramm, G. 1956. Infectivity of ribonucleic acid from tobacco mosaic virus. —Nature 177: 702–703.
Halvorson, H. O., Vary, J. C. and Steinberg, W. 1966. Developmental changes during the formation and breaking of dormant states in bacteria.—Annu. Rev. Microbiol. 20: 169–188.
Henney, H. and Storck, R. 1963a. Ribosomes and ribonucleic acids in three morphological states of Neurospora.—Science 142: 1675–1677.
Henney, H. and Storck, R. 1963b. Nucleotide composition and ribonucleic acids from Neurospora crassa.—J. Bacteriol. 85: 822–826.
Huang, R. C. C., Bonner, J. and Murray, K. 1964. Physical and biological properties of soluble nucleohistones.—J. Mol. Biol. 8: 54–64.
Hollomon, D. W. 1969. Biochemistry of germination in Peronospora tabacina (Adam) conidia: Evidence for the existence of stable messenger RNA.—J. Gen. Microbiol. 55: 267–274.
Lin, F. K., Davies, F. L., Tripathi, R. K., Raghu, K. and Gottlieb, D. 1971. Ribonucleic acids in spore germination of Ustilago maydis.—Phytopathology 61: 645–648.
Linskens, H. F. 1964. Isolation of ribosomes from pollen.—Planta 73: 194–200.
Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. 1951. Protein measurement with the Folin phenol reagent.—J. Biol. Chem. 193: 265–275.
Mandell, J. D. and Hershey, A. D. 1960. A fractionating column for analysis of nucleic acids.—Anal. Biochem. 1: 66–77.
Moyer, R. C. and Storck, R. 1964. Properties of ribosomes and RNA from Aspergillus niger. —Arch. Biochem. Biophys. 104: 193–201.
Ono, T., Kimura, K. and Yanagita, Y. 1966. Sequential synthesis of various molecular species of ribonucleic acid in the early phase of conidia germination in Aspergillus oryzae. —J. Gen. Appl. Microbiol. 12: 13–26.
Schneider, W. C. 1957. Determination of nucleic acids in tissues by pentose analysis, p. 680–684. In S. P. Colowick and N. O. Kaplan (eds.), Methods in Enzymology, Vol. III. —Academic Press, New York.
Schultz, G. A., Chen, D. and Katchalski, E. 1972. Localization of a messenger RNA in a ribosomal fraction from ungerminated wheat embryos.—J. Mol. Biol. 66: 379–390.
Seshachalam, D. and Lauritis, J. A. 1970. Characterization of abnormal RNA in Ustilago maydis.—Proc. Indian Sci. Congr. 58th, Part III: 426.
Slater, D. W. and Spiegelman, S. 1966. A chemical and physical characterization of echinoid RNA during early embryogenesis.—Biophys. J. 6: 385–404.
Spirin, A. S. 1969. Informosomes.—Europ. J. Biochem. 10: 20–35.
Staples, R. C., Bedigian, D. and Williams, P. H. 1968. Evidence for polysomes in extracts of bean rust uredospores.—Phytopathology 58: 151–154.
Tanaka, K., Ono, T. and Yanagita, Y. 1966. Further observations on the carbon dioxide incorporation into RNA in the early phase of conidia germination in Aspergillus oryzae with special reference to soluble RNA synthesis.—J. Gen. Appl. Microbiol. 12: 329–336.
Van Etten, J. L. 1969. Protein synthesis during fungal spore germination.—Phytopathology 59: 1060–1064.
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Seshachalam, D. A protein-nucleic acid complex in teliospores of Ustilago maydis . Antonie van Leeuwenhoek 40, 265–274 (1974). https://doi.org/10.1007/BF00394384
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DOI: https://doi.org/10.1007/BF00394384