Skip to main content
SpringerLink
Log in

Nucleotide sequences of cDNA clones encoding the entire precursor polypeptide for subunit VI and of the plastome-encoded gene for subunit VII of the photosystem I reaction center from spinach

  • Original Articles
  • Published:
Current Genetics Aims and scope Submit manuscript

Summary

Recombinant phage which encode the entire precursor polypeptide for subunit VI of the photosystem I reaction center have been selected from a lambda gt11 cDNA expression library made from polyadenylated RNA of spinach seedlings. The sequence predicts a precursor polypeptide of 144 amino acids (Mr = 15.3 kDa), a mature protein of 95 residues (Mr = 10.4 kDa) that lacks methionine, histidine and cysteine, and a transit peptide of 49 residues (Mr = 4.9 kDa). The corresponding gene(s) is (are) designated psaH. The gene for subunit VII, psaC, has been located in the small single-copy region of the spinach plastid chromosome using a synthetic oligonucleotide and a heterologous hybridization probe. It is part of a polycistronic transcription unit that is constitutively expressed and processed. Putative processing products include a monocistronic RNA for psaC. The polypeptide chain of 81 (deduced) amino acids is highly conserved and strikingly resembles bacterial-type ferredoxins. It harbours cysteine residues that appear to be involved in the ligation of the two 4Fe4S centres A and B in photosystem I. None of the two subunits appears to be membrane-spanning, and subunit VI, as subunit VII, is located at the reducing (stromal) side of the reaction center. All available information on the major subunits of photosystem I from spinach has been combined into a (revised) topographic model. Evidence that the innermost — plastome-encoded — core of photosystem I represents an old bacterial heritage in present day chloroplasts is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adman ET, Sicker LC, Jensen LH (1973) J Biol Chem 248:3987–3996

    Google Scholar 

  • Alt J, Westhoff P, Sears BB, Nelson N, Hurt E, Hauska, Herrmann RG (1983) EMBO J 2:979–986

    Google Scholar 

  • Bassi R, Simpson D (1987) Eur J Biochem 163:221–230

    Google Scholar 

  • Bengis C, Nelson N (1975) J Biol Chem 250:2783–2788

    Google Scholar 

  • Bengis C, Nelson N (1977) J Biol Chem 252:4564–4569

    Google Scholar 

  • Birnboim HC, Doly J (1979) Nucleic Acids Res 7:1513–1523

    Google Scholar 

  • Buchanan BB (1980) Annu Rev Plant Physiol 31:341–374

    Google Scholar 

  • Buchanan BB, Evans MCW (1969) Biochim Biophys Acta 180:123–129

    Google Scholar 

  • Carillo N, Seyer P, Tyagi A, Herrmann RG (1986) Curr Genet 10:619–624

    Google Scholar 

  • Dunn PPJ, Gray JC (1988) Plant Mol Biol 11:311–319

    Google Scholar 

  • Dunn PPJ, Packman LC, Pappin D, Gray JC (1988) FEBS Lett 228:157–161

    Google Scholar 

  • Eckerskorn C, Mewes W, Goretzki H, Lottspeich F (1988) Eur J Biochem 176:509–519

    Google Scholar 

  • Fish LE, Kück U, Bogorad L (1985) J Biol Chem 260:1413–1421

    Google Scholar 

  • Flinta C, Persson B, Jörnvall H, Heijne G von (1986) Eur J Biochem 154:193–196

    Google Scholar 

  • Fukuyama K, Nagahara Y, Tsukihara T, Katsube Y, Hase T, Matsubara H (1988) J Mol Biol 199:183–193

    Google Scholar 

  • Golbeck JH, Parrett K, McDermott AE (1987) Biochim Biophys Acta 893:149–160

    Google Scholar 

  • Hayashida N, Matsubayashi T, Shinozaki K, Sugiura M, Inove K, Hiyama T (1987) Curr Genet 12:247–250

    Google Scholar 

  • Herrmann RG, Westhoff P, Alt J, Tittgen J, Bisanz C, Sears BB, Nelson N, Hurt E, Hauska, Viebrock A, Sebald W (1983) In: Cifferi O, Dure III L (eds) Structure and function of plant genomes. Plenum Publ Corp, New York, pp 143–154

    Google Scholar 

  • Herrmann RG, Westhoff P, Alt J, Tittgen J, Nelson N (1985) In: van Vloten-Doting L, Groot SP, Hall TC (eds) Molecular form and function of plant genomes. Plenum Publ Corp, New York, pp 233–256

    Google Scholar 

  • Hippler M, Ratajczak R, Haehnel W (1989) FEBS Lett (in press)

  • Hoffman NE, Pichersky E, Malik VS, Ko K, Cashmore AR (1988) Plant Mol Biol 10:435–445

    Google Scholar 

  • Høj PB, Svendsen I, Scheller HV, Møller BL (1987) J Biol Chem 262:12676–12684

    Google Scholar 

  • Hosler JP, Yocum CF (1987) Plant Physiol 83:965–969

    Google Scholar 

  • Howarth AJ, Gardner RC, Messing J, Shepheard RJ (1981) Virology 112:678–685

    Google Scholar 

  • Jansen T, Rother C, Steppuhn J, Reinke H, Beyreuther K, Jansson C, Andersson B, Herrmann RG (1987) FEBS Lett 216:234–240

    Google Scholar 

  • Jansen T, Reiländer H, Steppuhn J, Herrmann RG (1988) Curr Genet 13:517–522

    Google Scholar 

  • Kamo M, Tsugita A, Wiessner C, Wedel N, Bartling D, Herrmann, RG, Aguilar F, Gardet-Sabri L, Schürmann P (1989) Eur J Biochem (in press)

  • Kirsch W, Seyer P, Herrmann RG (1986) Curr Genet 10:843–855

    Google Scholar 

  • Kozak M (1987) J Mol Biol 196:947–950

    Google Scholar 

  • Kyte J, Doolittle RF (1982) J Mol Biol 157:105–132

    Google Scholar 

  • Lagoutte B (1988) FEBS Lett 232:275–280

    Google Scholar 

  • Lehmbeck J, Rasmussen OF, Bookjans GB, Jepsen BR, Stummann BM, Henningsen KW (1986) Plant Mol Biol 7:3–10

    Google Scholar 

  • Lütcke HA, Chow KC, Michel FS, Moss KA, Kern HF, Scheele GA (1987) EMBO J 6:43–48

    Google Scholar 

  • Malkin R (1987) In: Barber J (ed) The light reactions. Elsevier, Amsterdam, New York, pp. 495–525

    Google Scholar 

  • Malkin R, Aparicio PJ, Arnon DI (1974) Proc Natl Acad Sci USA 71:2362–2366

    Google Scholar 

  • Matsubara M, Hase T (1983) In: Jensen U, Fairbrothers DE (eds) Proteins and nucleic acids in plant systematics. Springer, Berlin, Heidelberg, New York, pp 169–181

    Google Scholar 

  • Melton DA, Krieg PA, Rebagliati MR, Maniatis T, Zinn K, Green MR (1984) Nucleic Acids Res 12:7035–7056

    Google Scholar 

  • Merati G, Zanetti G (1987) FEBS Lett 215:37–40

    Google Scholar 

  • Michel H, Deisenhofer J (1988) Biochemistry 27:2–7

    Google Scholar 

  • Mullet JE, Burke JJ, Arntzen CJ (1980) Plant Physiol 65:814–822

    Google Scholar 

  • Mullet JE, Grossmann AR, Chua NH (1981) Cold Spring Harbor Symp Quant Biol 46:979–984

    Google Scholar 

  • Münch S, Ljungberg U, Steppuhn J, Schneiderbauer A, Nechushtai R, Beyreuther K, Herrmann RG (1988) Curr Genet 14:511–518

    Google Scholar 

  • Nanba O, Satoh K (1987) Proc Natl Acad Sci USA 84:109–112

    Google Scholar 

  • Nechushtai R, Nelson N (1981a) J Bioenerg Biomem 13:295–306

    Google Scholar 

  • Nechushtai R, Nelson N (1981b) J Biol Chem 256:11624–11628

    Google Scholar 

  • Nechushtai R, Nelson N (1985) Plant Mol Biol 4:377–384

    Google Scholar 

  • Nechushtai R, Nelson N, Mattoo AK, Edelmann M (1981) FEBS Lett 125:115–119

    Google Scholar 

  • Nelson N (1987) In: Amesz J (ed) New comprehensive biochemistry: photosynthesis. Elsevier Science Publ, Amsterdam, pp 213–231

    Google Scholar 

  • Nitschke W, Feiler U, Lockau W, Hauska (1987) FEBS Lett 218:283–286

    Google Scholar 

  • Obokata J (1986) Plant Physiol 81:705–707

    Google Scholar 

  • Oh-oka H, Takahashi Y, Wada K, Matsubara H, Ohyama K, Ozeki H (1987) FEBS Lett 218:52–54

    Google Scholar 

  • Oh-oka H, Takahashi Y, Kuriyama K, Saeki K, Matsubara H (1988) J Biochem 103:962–968

    Google Scholar 

  • Ohyama K, Fukuzawa H, Kohchi T, Shirai H, Sano T, Sano S, Umesono K, Shiki Y, Takeuchi M, Chang Z, Aota S, Inokuchi H, Ozeki H (1986) Nature 322:572–574

    Google Scholar 

  • Ortiz W, Lam E, Ghirardi M, Malkin R (1984) Biochim Biophys Acta 776:505–509

    Google Scholar 

  • Ovchinnikov YA, Abdulaev NG, Shmuckler BE, Zargarov AA, Kutuzov MA, Telezhinskaya IN, Levina NB, Zolotarev AS (1988) FEBS Lett 232:364–368

    Google Scholar 

  • Proudfoot N (1982) Nature 298:516–517

    Google Scholar 

  • Rao KK, Hall DO (1977) In: Leigh GJ (ed) The evolution of metalloenzymes, metalloproteins and related materials. Symposium Press, London, pp 39–65

    Google Scholar 

  • Robinson C, Ellis RJ (1984) Eur J Biochem 142:337–342

    Google Scholar 

  • Rother C, Jansen T, Tyagi A, Tittgen J, Herrmann RG (1986) Curr Genet 11:171–176

    Google Scholar 

  • Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467

    Google Scholar 

  • Schantz R, Bogorad L (1988) Plant Mol Biol 11:239–247

    Google Scholar 

  • Scheller HV, Svendsen I, Møller BL (1989) J Biol Chem 264:6929–6934

    Google Scholar 

  • Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, YamaguchiShinozaki K, Ohto C, Torozawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H, Sugiura M (1986) EMBO J 5:2043–2049

    Google Scholar 

  • Smeekens S, van Binsbergen J, Weisbeek P (1985) Nucleic Acids Res 13:3179–3194

    Google Scholar 

  • Smith A, Gray JC (1984) In: Sybesma C (ed) Advances in photosynthesis research, vol IV. Martinus Nijhoff, The Hague, pp 513–516

    Google Scholar 

  • Staehelin LA (1986) In: Staehelin LA, Arntzen CJ (eds) Encyclopedia of plant physiology: photosynthesis III. Springer, Berlin Heidelberg New York, pp 1–72

    Google Scholar 

  • Steppuhn J, Herrmann RG (1989) FEBS Lett (in press)

  • Steppuhn J, Hermans J, Nechushtai R, Ljungberg U, Thümmler F, Lottspeich F, Herrmann RG (1988) FEBS Lett 237:218–224

    Google Scholar 

  • Stout GH, Turley S, Sieker LC, Jensen LH (1988) Proc Natl Acad Sci USA 85:1020–1022

    Google Scholar 

  • Thornber JP (1986) In: Staehelin LA, Arntzen CJ (eds) Encyclopedia of plant physiology: photosynthesis III. Springer, Berlin Heidelberg New York, pp 98–135

    Google Scholar 

  • Tittgen J, Hermans J, Steppuhn J, Jansen T, Jansson C, Andersson B, Nechushtai R, Nelson N, Herrmann RG (1986) Mol Gen Genet 204:258–265

    Google Scholar 

  • Tyagi A, Hermans J, Steppuhn J, Jansson C, Vater F, Herrmann RG (1987) Mol Gen Genet 207:288–293

    Google Scholar 

  • Vallejos RH, Ceccarelli E, Chan R (1984) J Biol Chem 259:8048–8051

    Google Scholar 

  • Vierling E, Alberte RS (1983) Plant Physiol 72:625–633

    Google Scholar 

  • von Heijne G, Steppuhn J, Herrmann RG (1989) Eur J Biochem 180:535–545

    Google Scholar 

  • Wedel N, Bartling D, Herrmann RG (1988) Bot Acta 101:295–300

    Google Scholar 

  • Westhoff P, Herrmann RG (1988) Eur J Biochem 171:551–564

    Google Scholar 

  • Westhoff P, Alt J, Nelson N, Bottomley W, Bünemann H, Herrmann RG (1983) Plant Mol Biol 2:95–107

    Google Scholar 

  • Wynn RM, Malkin R (1988) Biochemistry 27:5863–5869

    Google Scholar 

  • Zanetti G, Merati G (1987) Eur J Biochem 169:143–146

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Botanisches Institut der Ludwig-Maximilians-Universität, Menzinger Strasse 67, D-8000, München 19, Germany

    J. Steppuhn, J. Hermann, G. S. Herrmann & R. G. Herrmann

  2. Botany Department, The Hebrew University, 91904, Jerusalem, Israel

    R. Nechushtai

Authors
  1. J. Steppuhn
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Hermann
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Nechushtai
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. S. Herrmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. R. G. Herrmann
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Dedicated to the 60th birthday of Prof. A. Trebst, Ruhr-Universität, Bochum, FRG

Rights and permissions

Reprints and permissions

About this article

Cite this article

Steppuhn, J., Hermann, J., Nechushtai, R. et al. Nucleotide sequences of cDNA clones encoding the entire precursor polypeptide for subunit VI and of the plastome-encoded gene for subunit VII of the photosystem I reaction center from spinach. Curr Genet 16, 99–108 (1989). https://doi.org/10.1007/BF00393402

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00393402

Key words

Search

Navigation