Abstract
Polyadenylated and nonpolyadenylated mRNa from the red algae Cyanidium caldarium Geitler and Porphyridium aerugineum Geitler were translated in a cell-free system. The α-and β-subunits of phycocyanin and allophycocyanin and also both subunits of ribulose-1,5-bisphosphate carboxylase-oxygenase (RuBPCase) were identified with the help of specific antibodies as translation products of non-polyadenylated mRNA. Both subunits of RuBPCase were synthesized with molecular weights in the range of the mature forms. This is in contrast to the findings with green algae and higher plants where the small subunits of RuBPCase are always made by polyadenylated mRNA in the form of a larger precursor molecule. We discuss our findings with regard to the systematic position of the algae used and the evolution of plastids.
Similar content being viewed by others
Abbreviations
- Poly(A)--RNA:
-
non-
- Poly(A)+-RNA:
-
polyadenylated RNA
- RuBPCase:
-
ribulose-1,5-bisphosphate carboxylase/oxygenase EC 4.1.1.39
- SDS:
-
sodium dodecyl sulfate
References
Allen, M.B. (1959) Studies with Cyanidium caldarium, au anomalously pigmented chlorophyte. Arch. Mikrobiol. 32, 270–277
Bonner, W.M., Laskey, R.A. (1974) A film detection method for Tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur. J. Biochem. 46, 83–88
Covey, S.N., Grierson, D. (1976) Subcellular distribution and properties of poly (A)-containing RNA from cultured plant cells. Eur. J. Biochem. 63, 599–606
Ellis, J. (1981) Chloroplast proteins: Synthesis, transport assembly. Annu. Rev. Plant Physiol. 32, 111–138
Ford, T.W. (1979) Ribulose-1,5-bisphosphate carboxylase from the thermophilic, acidophilic alga Cyanidium caldarium (Geitler). Purification, characterisation and thermostability of the enzyme. Biochim. Biophys. Acta 569, 239–248
Gantt, E. (1981) Phycobilisomes. Annu. Rev. Plant Physiol. 32, 327–347
Grossman, A., Bartlett, S., Schmidt, G., Mullet, Y., Chua, N.-H. (1982) Optimal conditions for post-translational uptake of proteins by isolated chloroplasts. J. Biol. Chem. 257, 1558–1563
Hager, D.A., Burgess, R.R. (1980) Elution of proteins from SDS-PAGE, removal of SDS and renaturation of enzyme activity, results with σ subunit of E. coli RNA-polymerase, wheat germ DNA topoisomerase and other enzyms. Anal. Biochem. 109, 76–86
Koller, K.P., Wehrmeyer, W. (1974) Isolierung und Charakterisierung der Biliproteide von Rhodella violacea (Bangiophycidae). Arch. Microbiol. 100, 253–270
Kremer, B.P., Feige, G.B. (1979) Accumulation of photoassimilatory products by phycobiliprotein-containing algae with special reference to Cyanidium caldorium. Z. Naturforsch. Teil C 34, 1209–1214
Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head bacteriophage T4. Nature (London) 227, 680–685
Nagashima, H., Fukuda, J. (1981) Low molecular weight carbohydrates in Cyanidium and some related algae. Phytochemistry 20, 439–442
Nelles, L.P., Bamburg, J.R. (1976) Rapid visualization of protein-dodecylsulfate complexes in polyacrylamide gels. Anal. Biochem. 73, 522–531
Parthier, B. (1982) The cooperation of nuclear and plastid genomes in plastid biogenesis and differentiation. Biochem. Physiol. Pflanz. 177, 283–317
Ramus, J. (1972) The production of extracellular polysaccharide by the unicellular red algae Porphyridium aerugineum. J. Phycol. 8, 97–111
Wallace, D.C. (1982) Structure and evolution of organelle genomes. Microbiol. Rev. 46, 208–240
Westhoff, P., Nelson, N., Bünemann, H., Herrmann, R.G. (1981a) Localization of genes for coupling factor subunits on the spinach plastid chromosome. Curr. Genet. 4, 109–120
Westhoff, P., Zetsche, K. (1981) Regulation of the synthesis of ribulose-1,5-bisphosphate carboxylase and its subunits in the Flagellate Chlorogonium elongatum. Different levels of translatable messenger RNAs for the large and the small subunits in autotrophic and heterotrophic cells as determined by immunological techniques. Eur. J. Biochem. 116, 261–267
Westhoff, P., Zimmermann, K., Boege, F., Zetsche, K. (1981b) Regulation of the synthesis of ribulose-1,5-bisphosphate carboxylase and its subunits in the Flagellate Chlorogonium elongatum. II. Coordinated synthesis of the large and small subunits. z. Naturforsch. Teil C 36, 942–950
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Steinmüller, K., Kaling, M. & Zetsche, K. In-vitro synthesis of phycobiliproteids and ribulose-1,5-bisphosphate carboxylase by non-poly-adenylated-RNA of Cyanidium caldarium and Porphyridium aerugineum . Planta 159, 308–313 (1983). https://doi.org/10.1007/BF00393168
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00393168