Abstract
Similar ranges of gibberellins (GAs) were detected by high-performance liquid chromatography (HPLC)-immunoassay procedures in ten cultures of wild-type and mutant strains of Rhizobium phaseoli. The major GAs excreted into the culture medium were GA1 and GA4. These identifications were confirmed by combined gas chromatographymass spectrometry. The HPLC-immunoassays also detected smaller amounts of GA9- as well as GA20-like compounds, the latter being present in some but not all cultures. In addition to GAs, all strains excreted indole-3-acetic acid (IAA) but there was no obvious relationship between the amounts of GA and IAA that accumulated. The Rhizobium strains studied included nod − and fix − mutants, making it unlikely that the IAA- and GA-biosynthesis genes are closely linked to the genes for nodulation and nitrogen fixation.
The HPLC-immunoassay analyses showed also that nodules and non-nodulated roots of Phaseolus vulgaris L. contained similar spectra of GAs to R. phaseoli culture media. The GA pools in roots and nodules were of similar size, indicating that Rhizobium does not make a major contribution to the GA content of the infected tissue.
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Abbreviations
- EIA:
-
enzyme immunoassay
- GAn :
-
gibberellin An
- GC-MS:
-
gas chromatography-mass spectrometry
- HPLC:
-
high-performance liquid chromatography
- IAA:
-
indole-3-acetic acid
- Me:
-
methyl ester
- RIA:
-
radioimmunoassay
- TLC:
-
thin-layer chromatography
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Atzorn, R., Crozier, A., Wheeler, C.T. et al. Production of gibberellins and indole-3-acetic acid by Rhizobium phaseoli in relation to nodulation of Phaseolus vulgaris roots. Planta 175, 532–538 (1988). https://doi.org/10.1007/BF00393076
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DOI: https://doi.org/10.1007/BF00393076