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Cell wall glycoproteins from Chlamydomonas reinhardii, and their self-assembly

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Abstract

We have investigated the in vitro reassembly of the salt soluble, hydroxyproline rich, glycoproteins from the cell wall of Chlamydomonas reinhardii, into structured cell wall fragments. We have devised an assay which has been used to follow the reassembly of the unfractionated and fractionated (2BI and 2BII) cell wall glycoproteins. Reassembly has a pH optimum of 5, a temperature optimum of 20°C, and the final size of the reassembled fragments appears to be promoted by the minor component 2BI. Periodate oxidation experiments show that sugar residues, in particular mannose, are important for accurate reassembly. Using electron microscopy, the structure of the reassembled products has been elucidated, as have intermediate stages in the reassembly process.

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Abbreviations

TRIS:

Tris(hydroxymethyl)-methylamine

SDS:

Sodium dodecyl sulphate

PAGE:

Polyacrylamide gel electrophoresis

References

  • Catt, J.W., Hills, G.J., Roberts, K.: A structural glycoprotein, containing hydroxyproline, isolated from the cell wall of Chlamydomonas reinhardii. Planta 131, 165–171 (1976)

    Google Scholar 

  • Clamp, J.R., Hough, L.: The periodate oxidation of amino acids with reference to studies on glycoproteins. Biochem. J. 94, 17–24 (1965)

    Google Scholar 

  • Dea, I.C.M., McKinnon, A.A. Rees, D.A.: Tertiary and quateruary structure in aqueous polysaccharide systems which model cell wall cohesion: Reversible changes in conformation and association of agarose, carrageenan and galactomannans. J. Mol. Biol. 68, 152–172 (1972)

    Google Scholar 

  • Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., Smith, F.: Colorimetric method for determination of sugars and related substances. Anal. Chem. 28, 350–356 (1956)

    Google Scholar 

  • Erickson, H.P., Klug, A.: Measurement and compensation of defocussing and aberrations by Fourier processing of electron micrographs. Phil. Trans. Roy. Soc. Lond. B. 261, 105–118 (1971)

    Google Scholar 

  • Fabergé, A.C., Oliver, R.M.: Methylamine tungstate, a new negative stain. J. Microscopie 20, 241–246 (1974)

    Google Scholar 

  • Gaskin, F., Cantor, C.R., Shelanski, M.L.: Turbidometric studies of the in vitro assembly and disassembly of porcine neurotubules. Berne B.: Appendix. Interpretation of the light scattering of long rods. J. Mol. Biol. 89, 737–758 (1974)

    Google Scholar 

  • Hills, G.J., Gurney-Smith, M., Roberts, K.: Structure, composition and morphogenesis of the cell wall of Chlamydomonas reinhardii. J. Ultrastruct. Res. 43, 179–192 (1973)

    Google Scholar 

  • Hills, G.J., Phillips, J.M., Gay, M.R., Roberts, K.: Self assembly of a plant cell wall in vitro. J. Mol. Biol. 96, 431–441 (1975)

    Google Scholar 

  • Kauss, H., Bowles, D.J.: Some properties of carbohydrate-binding proteins (lectins) solubilised from the cell walls of Phaseolus vulgaris. Planta 130, 169–174 (1976)

    Google Scholar 

  • Laemmli, U.K., Favre, M.: Maturation of the head of bacteriophage T4. J. Mol. Biol. 80, 575–599 (1973)

    Google Scholar 

  • Lamport, D.T.A.: Structure, biosynthesis and significance of cell wall glycoproteins. Rec. Adv. Phytoch. 11, 79–115 (1977)

    Google Scholar 

  • Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–278 (1951)

    Google Scholar 

  • Roberts, K.: Crystalline glycoprotein cell walls of algae: their structure, composition and assembly. Phil. Trans. Roy. Soc. Lond. B 268, 129–146 (1974)

    Google Scholar 

  • Roberts, K., Gurney-Smith, M., Hills, G.J.: Structure, composition and morphogeneis of the cell wall of Chlamydomonas reinhardii I. Ultrastructure and preliminary chemical analysis. J. Ultrastruct. Res. 40, 599–613 (1972)

    Google Scholar 

  • Roberts, K., Phillips, J.M., Hills, G.J.: Structure, composition and morphogenesis of the cell wall of Chlamydomonas reinhardii VI. The flagellar collar. Micron 5, 341–357 (1975)

    Google Scholar 

  • Smith, C.E., Lauffer, M.A.: Polymerisation — depolymerisation of TMV protein VIII. Light scattering studies. Biochemistry 6, 2457–2465

  • Sueoka, N.: Mitotic replication of deoxyribonucleic acid in Chlamydomonas reinhardii. Proc. Natl. Acad. Sci. USA 46, 83–91 (1960)

    Google Scholar 

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Author notes

  1. J. W. Catt

    Present address: Fachbereich Biologie der Universität, Postfach 3049, D-6750, Karserslautern, Federal Republic of Germany

Authors and Affiliations

  1. John Innes Institute, Colney Lane, NR4 7UH, Norwich, U.K.

    J. W. Catt, G. J. Hills & K. Roberts

Authors
  1. J. W. Catt
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  2. G. J. Hills
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  3. K. Roberts
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Additional information

This is the fifth paper in a series entitled “Structure composition and morphogenesis of the cell wall of Chlamydomonas reinhardii”. The last paper in this series was Catt et al. (1976)

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Catt, J.W., Hills, G.J. & Roberts, K. Cell wall glycoproteins from Chlamydomonas reinhardii, and their self-assembly. Planta 138, 91–98 (1978). https://doi.org/10.1007/BF00392922

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  • DOI: https://doi.org/10.1007/BF00392922

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