Abstract
Phospholipid multibilayers, obtained from two cultivars of thermally acclimated wheats of different frost resistances (Triticum aestivum L. cv. Penjamo 62, the sensitive cultivar, and T. aestivum L. cv. Miranovskaja 808, the frost-resistant cultivar), were investigated using electron-spin-resonance and X-ray-diffraction techniques. The former technique revealed two breaks in the motion of the spin-labelled fatty acid 2-(14-carboxyte-tradecyl)-2-ethyl-4,4-dimethyl-3-oxazolidinyloxyl, for both cultivars (+3°C,-17° C and +5° C,-18° C, respectively) when grown at 22° C. The resistant cultivar compensated for exposure to cold (+2° C) by shifting the onset of the apparent phase-separation temperature from +3° C to-16° C. The sensitive cultivar was unable to do so. X-ray diffraction did not reveal fluid-to-gel transitions between +20° C and-10° C in any of the samples. The possible role of the formation of relatively ordered aggregates or clusters of lipid molecules discerned by spin probe within the otherwise freely dispersed liquid-like lipids is discussed in terms of freezing injury of plants.
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Abbreviations
- ESR:
-
electron-spin resonance
- MIR:
-
cultivar Miranovskaja 808
- PEN:
-
cultivar Penjamo 62
References
Anderson, R.L., Minton, K.W., Li, G.C., Hahn, G.M. (1981) Temperature induced homeoviscous adaptation of Chinese hamster ovary cells. Biochim. Biophys. Acta 470, 395–411
Blazyk, J.F., Stein, J.M. (1972) Phase transitions in mammalian membranes. Biochim. Biophys. Acta 266, 737–741
Carter, J.V. (1980) Role of membrane lipid phase transitions in responses of plants to low temperature stress. Cryo-Letters 1, 408–416
Chapman, E., Wright, L.C., Raison, J.K. (1979) Seasonal changes in the structure and function of mitochondrial membranes of Artichoke tubers. Plant Physiol. 63, 363–366
Cossins, A.R. (1977) Adaptation of biological membranes to temperature. The effect of temperature acclimation of goldfish upon the viscosity of synaptosomal membranes. Biochim. Biophys. Acta 470, 395–411
Dickens, B.F., Thompson, G.A., Jr. (1981) Rapid membrane response during low-temperature acclimation, correlation of early changes in the physical properties and lipid composition of Tetrahymena microsomal membranes. Biochim. Biophys. Acta 664, 211–218
Dickens, B.F., Thompson, G.A., Jr. (1982) Phospholipid molecular species alterations in microsomal membranes as an initial key step during cellular acclimation to low temperature, Biochemistry 21, 3604–3611
Engelman, D.M. (1971) Lipid bilayer structure in the membrane of Mycoplasma laidlawii. J. Mol. Biol. 58, 153–165
Horváth, I., Vigh, L., Belea, A., Farkas, T. (1979) Conversion of phosphatidyl choline to phosphatidic acid in freeze injured rye and wheat cultivars. Physiol. Plant. 45, 57–62
Horváth, I., Vigh, L., Belea, A., Farkas, T. (1980) Hardiness dependent accumulation of phospholipids in leaves of wheat cultivars. Physiol. Plant. 49, 117–120
Horváth, I., Vigh, L., Woltjes, J., van Hasselt, P.R., Kuiper, P.J.C. (1982) The physical state of lipids of the leaves of cucumber genotypes as affected by temperature. In: Biochemistry and Metabolism of Plant Lipids, vol. 8, pp. 427–431, Wintermans, J.F.G.M., Kuiper, P.J.C., eds. Elsevier Biomedical Press, Amsterdam New York Oxford
Jensen, M., Heber, U., Oettmeier, W. (1981) Chloroplast membrane damage during freezing: the lipid phase. Cryobiology 18, 322–335
Kahovcova, J., Odavic, R. (1969) A simple method for the quantitative analysis of phospholipids separated by thin layer chromatography. J. Chromatogr. 40, 90–96
Lee, A.G. (1977) Lipid phase transitions and phase diagrams. I. Lipid phase transitions. Biochim. Biophys. Acta 472, 237–281
Lynch, D., Thompson, G.A., Jr. (1984) Retailored lipid molecular species: a tactical mechanism for modulating membrane properties. Trends Biochem. Sci. 9, 442–445
Martin, C.E., Siegel, D., Aaronson, L.S. (1981) Effect of temperature acclimation on Neurospora phospholipids. Fatty acid desaturation appears to be a key element in modifying phospholipid fluid properties. Biochim. Biophys. Acta 665, 399–407
Martonosi, M.A. (1974) Thermal analysis of sarcoplasmic reticulum membranes. FEBS Lett. 47, 327–329
Pomeroy, M.K., Raison, J.K. (1981) Maintenance of membrane fluidity during development of freezing tolerance of winter wheat seedlings. Plant Physiol. 68, 382–385
Raison, J.K., Wright, L.C. (1983) Thermal phase transitions in the polar lipids of plant membranes. Their induction by desaturated phospholipids and their possible relation to chilling injury. Biochim. Biophys. Acta. 731, 69–78
Rottem, S., Hubbel, W.L., Hayflick, L., McConnell, H.M. (1970) Motion of fatty acid spin labels in the plasma membrane of Mycoplasma. Biochim. Biophys. Acta 219, 104–113
Rouser, G., Fleischer, S., Yamamoto, A. (1970) Two dimensional thin-layer chromatographic separation of polar lipids and determination of phospholipid phosphorus analysis of spots. Lipids 5, 494–496
Sinensky, M. (1974) Homeoviscous adaptation — a homeostatic process that regulates the viscosity of membrane lipids in Escherichia coli. Proc. Natl. Acad. Sci. USA 71, 522–525
Steim, J.M., Tourtellotte, M.E., Reinert, J.G., McElhahey, R.N., Rader, R.L. (1969) Calorimetric evidence for the liquid-crystalline state of lipids in a biomembrane. Proc. Natl. Acad. Sci. USA 63, 104–109
Tsukamoto, Y., Ueki, T., Mitsui, T., Ono, T.A., Murata, N. (1980) Relationship between growth temperature of Anacystis nidulans and phase transition temperature of its thylakoid membranes. Biochim. Biophys. Acta 602, 673–675
Vigh, L. (1982) Adaptation of thylakoid membranes of wheat seedlings to low temperature. In: Biochemistry and Metabolism of Plant Lipids, vol. 8, pp. 401–414, Wintermans, J.F.G.M., Kuiper, P.J.C., eds. Elsevier Biomedical Press, Amsterdam New York Oxford
Vigh, L., Horváth, I., Horváth, L.I., Dudits, D., Farkas, T. (1979) Protoplast plasmalemma fluidity of hardened wheats correlates with frost resistance. FEBS Lett. 107, 291–294
Vigh, L., Huitema, H., Woltjes, J., van Hasselt, P.R. (1986) Drought stress-induced changes in the composition and physical state of phospholipids in wheat. Physiol. Plant. 67, 92–96
Willcox, M.E., Patterson, B.D. (1979) Breaks or curves? A visual aid to the interpretation of data. In: Low temperature stress in crop plants. The role of the membrane, pp. 523–525, Lyons, J.M., Graham, D., Raison, J.K., eds. Academic Press, London New York
Willemot, C., Pelletier, P. (1979) Effect of drought on frost resistance and fatty acid content of young winter wheat plants. Can. J. Plant Sci. 59, 639–643
Wunderlich, F., Ronai, A., Speth, V., Seeling, I., Blume, A. (1975) Thermotropic lipid clustering in Tetrahymena membranes. Biochemistry 14, 3730–3735
Yamada, Y., Hara, Y., Katagi, H., Senda, M. (1980) Protoplast fusion. Effect of low temperature on the membrane fluidity of cultured cells. Plant Physiol. 65, 1099–1102
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Vigh, L., Horváth, I., Woltjes, J. et al. Combined electron-spin-resonance, X-ray-diffraction studies on phospholipid vesicles obtained from cold-hardened wheats. Planta 170, 14–19 (1987). https://doi.org/10.1007/BF00392375
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DOI: https://doi.org/10.1007/BF00392375