Abstract
Urease (EC 3.5.1.5) is abundantly present in the seeds of many species of Leguminosae. There is at present conflicting information in the literature about its subcellular location and status as a glycoprotein. We have made a study of the subcellular location of urease in jack-bean cotyledons using an immunocytochemical approach; in addition, we studied the biosynthesis and glycoprotein nature of the enzyme using several biochemical approaches. All the results are in agreement with the interpretation that the seed urease is not a glycoprotein, is synthesized on free polysomes, and is present in the cytosol of the storage parenchyma cells.
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Abbreviations
- ConA:
-
Concanavalin A
- ER:
-
endoplasmic reticulum
- IgG:
-
immunoglobulin G
- Mr :
-
relative molecular mass
- SDS-PAGE:
-
sodium dodecyl sulfate-polyacrylamide gel electrophoresis
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On leave from Laboratoire de Photobiologie (CNRS-UA 203), Faculté des Sciences de Rouen, F-76130 Mont Saint Aignan, France
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Faye, L., Greenwood, J.S. & Chrispeels, M.J. Urease in jack-bean (Canavalia ensiformis (L.) DC) seeds is a cytosolic protein. Planta 168, 579–585 (1986). https://doi.org/10.1007/BF00392279
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DOI: https://doi.org/10.1007/BF00392279