Summary
Perception of polarized light inducing phytochrome-mediated polarotropism in protonemata of the fern Adiantum capillus-veneris L. was analyzed using brief microbeam irradiation with polarized red (R) or far-red light (FR). The polarotropic response inducible by irradiation of the subapical 10–30-μm part with polarized R vibrating parallel to the cell axis was nullified by subsequently giving R at the apical 0–2.5-μm region. This inhibitory effect of R showed an action dichroism, that is, polarized R vibrating normal to the cell axis was effective but the parallel-vibrating R was not. On the other hand, FR irradiation of the extreme tip after irradiation of the whole cell with depolarized R effectively induced a tropic response. This FR effect also showed action dichroism, with parallel-vibrating polarized FR being more effective than FR vibrating normal to the cell axis. When the apical-dome region and the adjacent subapical 10–20-μm region were sequentially irradiated with polarized R vibrating obliquely in different directions, polarotropism took place depending on the vibrating direction of the light given to the apical-dome region. Obliquely vibrating polarized FR given to the apical dome after irradiation of the whole cell with depolarized R also induced polarotropism. Thus, the difference in amount (or percent) of the far-redabsorbing form of phytochrome (Pfr) between the extreme tip and the subapical region appears to be crucial in regulating the direction of apical growth; the difference in Pfr level between the two sides of the protonemal apex may occur mainly at the apical dome. Furthermore, the transition moments of the red-absorbing form of phytochrome (Pr) and Pfr seem to be aligned parallel and normal, respectively, to the cell surface at the periphery of the apical hemisphere.
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Abbreviations
- FR:
-
far-red light
- Pfr :
-
far-red-absorbing form of phytochrome
- Pr :
-
red-absorbing form of phytochrome
- R:
-
red light
References
Bünning, E., Etzold, H. (1958) Über die Wirkung von polarisiertem Licht auf keimende Sporen von Pilzen, Moosen und Farnen. Ber. Dtsch. Bot. Ges. 71, 304–306
Davis, B.D. (1975) Bending growth in fern gametophyte protonema. Plant Cell Physiol. 16, 537–541
Etzold, H. (1965) Der Polarotropismus und Phototropismus der Chloronemen von Dryopteris filix mas (L.) Schott. Planta 64, 254–280
Haupt, W., Mörtel, G., Winkelnkemper, I. (1969) Demonstration of different orientation of phytochrome Pr and Pfr. Planta 88, 183–186
Kadota, A., Furuya, M. (1977) Apical growth of protonemata in Adiantum capillus-veneris. I. Red far-red reversible effect on growth cessation in the dark. Dev. Growth Differ. 19, 357–365
Kadota, A., Wada, M., Furuya, M. (1982) Phytochrome-mediated phototropism and different dichroic orientation of Pr and Pfr in protonemata of the fern Adiantum capillus-veneris L. Photochem. Photobiol. 35, 533–536
Kadota, A., Koyama, M., Wada, M., Furuya, M. (1984) Action spectra for polarotropism and phototropism in protonemata of the fern Adiantum capillus-veneris. Physiol. Plant. 61, 327–330
Murashige, T., Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497
Wada, M., Furuya, M. (1970) Photocontrol of the orientation of cell division in Adiantum. I. Effects of the dark and red periods in the apical cell of gametophytes. Dev. Growth Differ. 12, 109–118
Wada, M., Furuya, M. (1978) Effects of narrowbeam irradiations with blue and far-red light on the timing of cell division in Adiantum gametophytes. Planta 138, 85–90
Wada, M., Kadota, A., Furuya, M. (1981) Intracellular photoreceptive site for polarotropism in protonema of the fern Adiantum capillus-veneris L. Plant Cell Physiol. 22, 1481–1488
Wada, M., Kadota, A., Furuya, M. (1983) Intracellular localization and dichroic orientation of phytochrome in plasma membrane and/or ectoplasm of a centrifuged protonema of fern Adiantum capillus-veneris L. Plant Cell Physiol. 24, 1441–1447
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Kadota, A., Wada, M. & Furuya, M. Phytochrome-mediated polarotropism of Adiantum capillus-veneris L. protonemata as analyzed by microbeam irradiation with polarized light. Planta 165, 30–36 (1985). https://doi.org/10.1007/BF00392208
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DOI: https://doi.org/10.1007/BF00392208