Summary
We isolated a carrot cell mutant resistant to 8-azaguanine. Into this line a second mutation for resistance to α-amanitin was introduced. The double mutant was shown to be selectable in α-amanitin and counter-selectable in HAT medium. Resistance to Azg and sensitivity to HAT were correlated with an alteration of the enzyme HPRT. The properties of the double mutant as universal hybridizer were verified in a fusion with wild-type carrot protoplasts.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- Azg:
-
8-azaguanine
- HAT:
-
Hypoxanthine, aminopterin, thymidine
- HPRT:
-
Hypoxanthine-phosphoribosyl transferase
- PE:
-
Plating efficiency
- wt:
-
Wild-type
- 6TG:
-
6-thioguanine
References
Bright SWJ, Northcote DH (1975) A deficiency of hypoxanthine phosphoribosyl transferase in a sycamore callus resistant to azaguanine. Planta 123: 79–89
Caskey CT, Kruh GD (1979) The HPRT locus. Cell 16: 1–9
Cella R, Parisi B, Nielsen E (1982) Characterization of a carrot cell line resistant to azetidine-2-carboxylic acid. Plant Sc Lett 24: 135–135
Debatisse M, Berry M, Buttin G (1981) The potentiation of adenine toxicity to Chinese hamster cells by coformycin: suppression in mutants with altered regulation of purine biosynthesis or increased adenylate deaminase activity. J Cell Physiol 106: 1–11
Horsh RB, Jones GE (1978) 8-Azaguanine-resistant variants of cultured cells in Haplopappus gracilis. Can J Bot 56: 2660–2665
Kao KN, Michayluk MR (1974) A method for high frequency intergeneric fusion of plant protoplasts. Planta 115: 355–367
Lescure AM (1973) Selection of markers of resistance to base analogue in somatic cell cultures of Nicotiana tabacum. Plant Sc Lett 1: 375–383
Lo Schiavo F, Nuti Ronchi V, Terzi M (1980) Genetic effects of Griseofulvin on plant cell cultures. Theor Appl Genet 58:43–47
Lo Schiavo F, Biasini G, Giuliano G, Terzi M (1981) Development of a selective system for somatic cell hybrids. In: Induced mutations — a tool in plant breeding. IAEA, Vienna, pp 363–372
Oostindier-Braaksma FJ, Feenstra WJ (1973) Isolation and characterization of chlorate resistant mutants of Arabidopsis thaliana. Mutation Res 19: 175–185
Simon AE, Taylor MW, Bradley WEC, Thompson LH (1982) Model involving gene inactivation in the generation of autosomal recessive mutants in mammalian cells in culture. Mol Cell Biol 2: 1126–1133
Szybalski W, Szybalska EH, Ragni G (1962) Genetic studies with human cell lines. Natl Cancer Inst Monogr 7: 75–89
Terzi M, Giuliano G, Lo Schiavo F, Nuti Ronchi V (1982) Studies on plant cells showing temperature sensitive embryogenesis. In: Burger MM (ed) Embryonic Development. Part B: Cellular Aspects. Alan R Liss, Inc, New York
Van Diggelen OP, Donahue TF, Shin S (1979) Basis for differential cellular sensitivity to 8-azaguanine and 6-thioguanine. J Cell Physiol 98: 59–72
Vergara MR, Biasini G, Lo Schiavo F, Terzi M (1982) Isolation and characterization of carrot cell mutants resistant to α-amanitin. Z Pflanzenphysiol 107: 313–319
Author information
Authors and Affiliations
Additional information
Communicated by J. Schell
Rights and permissions
About this article
Cite this article
LoSchiavo, F., Giovinazzo, G. & Terzi, M. 8-azaguanine resistant carrot cell mutants and their use as universal hybridizers. Mol Gen Genet 192, 326–329 (1983). https://doi.org/10.1007/BF00392170
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00392170