Summary
An association is reported between an albuminlike protein (b-70) and the semidominant locus fluory-2 (fl2) which reduces the level of zein polypeptides in the maize endosperm. The protein b-70 is present at low level in wild-type endosperms and derppressed in fl2 endosperms. A correlation between the doses of the fl2 allele and the b-70 level has been found. Moreover a concomitant loss of the regulatory role of fl2 on zein level and on b-70 overproduction is evident when fl2 is genetically associated with o2 and o7, two recessive alleles of other zein regulatory loci. Protein b-70 is located on the membrane of the protein body where zein polypeptides accumulate. The existence of a functional relationship between this protein and the zein-secretory system is suggested or, as an alternative, that b-70 is a type of storage protein different from zeins, repressed in normal endosperms and derepressed by the fl2 allele.
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Abbreviations
- DAP:
-
days after pollination
- ER:
-
endoplasmic reticulum
- RER:
-
rough endoplasmic reticulum
- DTT:
-
dithiothreitol
- EDTA:
-
ethylene-diamintetra-acetate
- NADH:
-
nicotinamide-adenine dinucleotide, reduced
- PMSF:
-
phenylmethylsulfonyl-fluoride
- SDS:
-
sodium dodecylsulfate
- PAGE:
-
polyacrylamide gel electrophoresis
- PBS:
-
phosphate buffered saline (0.15 M NaCl, 0.01 M Na phosphate, pH 6.8)
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Communicated by H. Saedler
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Galante, E., Vitale, A., Manzocchi, L. et al. Genetic control of a membrane component and zein deposition in maize endosperm. Mol Gen Genet 192, 316–321 (1983). https://doi.org/10.1007/BF00392168
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DOI: https://doi.org/10.1007/BF00392168