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Comparison of DNA-incising capacities in fibroblast strains from the Mannheim XP collection after treatment with N-acetoxy-2-acetylaminofluorene and UV light

  • Original Papers
  • Experimental Oncology
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Summary

The DNA-incising capacity was determined in 8 normal and 23 XP fibroblast strains of the Mannheim XP collection using the alkaline elution technique after treatment with both UV light and the “UV-like” carcinogen (Ac)2ONFln. Experimental conditions were chosen to allow for selective monitoring of repair-specific enzyme-catalyzed breaks. In order to compare DNA-incising capacities of the various cell strains after UV irradiation with those after treatment with (Ac)2ONFln, dose-response experiments including up to 8 dose levels were performed. The elution curves were analyzed by linear regression analysis. Elution velocities (in terms of DNA singlestrand breaks per 106 nucleotides) were plotted against the square root of the doses. The slope of the resulting regression line yielded a characteristic term, designated E 0, for the DNA-incising capacity of each cell strain. In contrast to normal fibroblasts, E 0 was found to be reduced in all XP cell strains belonging to the complementation groups A, C, D, E, F (or G) and I investigated, after treatment with both UV light or (Ac)2ONFln. Surprisingly, XP variant strains also exhibited lower E 0 values. A comparison of post-UV with post-(Ac)2ONFln DNA-incising capacities revealed that reduction in the E 0 values was very similar in all XP cell strains tested. These data suggest that the sensitivity of XP cells towards UV light or (Ac)2ONFln is due to the same enzymatic defect, namely impaired incision of DNA containing pyrimidine dimers or (Ac)2ONFln-DNA adducts.

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Abbreviations

XP:

xeroderma pigmentosum

(Ac)2ONFln:

N-acetoxy-2-acetylaminofluorene

HEPES:

N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid

ara-C:

1-β-d-arabinofuranosyl cytosine

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Authors and Affiliations

  1. Institute of Biochemistry, German Cancer Research Centre, Im Neuenheimer Feld 280, D-6900, Heidelberg, Federal Republic of Germany

    Odilia Popanda & Heinz Walter Thielmann

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  1. Odilia Popanda
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  2. Heinz Walter Thielmann
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Dedicated to Prof. W. Kunz on the occasion of his 65th birthday

This work was supported by the Deutsche Forschungsgemeinschaft, SFB 136

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Popanda, O., Thielmann, H.W. Comparison of DNA-incising capacities in fibroblast strains from the Mannheim XP collection after treatment with N-acetoxy-2-acetylaminofluorene and UV light. J Cancer Res Clin Oncol 114, 459–467 (1988). https://doi.org/10.1007/BF00391492

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  • DOI: https://doi.org/10.1007/BF00391492

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