Summary
Restriction mapping and DNA sequencing were used to characterize dispersed repetitive DNA in the chloroplast genome of Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco]. To map repeat families, chloroplast DNA (cpDNA) clones were hybridized at high stringency to one another and to cpDNA cut with restriction enzymes. Repeats are clustered in four regions of the genome and comprise at least six families. Sequence analysis of one repeat family shared among three XbaI fragments indicated the presence of a 633 by inverted repeat which contains a complete tRNA-Serine (GCU) gene and a highly conserved open reading frame (ORF 3.6). Both ends of this 633 bp dispersed repeat have a transposon-like combination of short direct and inverted repeats. One copy of the repeat flanks one of the endpoints of a major inversion which differentiates Douglas-fir from tobacco cpDNA. Dispersion of repetitive DNA by transposition, coupled with loss of the large inverted repeat, appears to have predisposed conifer cpDNA to a number of inversions. An 8 by (CATCTTTT) direct repeat in tobacco is located between two inverted sections in Douglas-fir; it may be a target sequence for homologous recombination.
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References
Axelrod DI (1986) Ann Missouri Bot Gard 73:565–641
Bowman CM, Barker RF, Dyer TA (1988) Curr Genet 14:127–136
Doring H-P, Stringer P (1986) Annu Rev Genet 20:175–200
Feinberg AP, Vogelstein B (1984) Anal Biochem 137:266–267
Galas DJ, Chandler M (1989) In: Berg DE, Howe MM (eds) Mobile DNA. Am Soc Microbiol Washington, DC, pp 109–162
Grindley NDF, Reed RR (1985) Annu Rev Biochem 54:863–96
Hermann RK (1985) The Genus Pseudotsuga: Ancestral History and Past Distribution. (Spec 2b) Forest Research Laboratory, Oregon State University, Corvallis
Howe CJ (1985) Curr Genet 10:139–145
Howe CJ, Barker RF, Bowman CM, Dyer TA (1988) Curr Genet 13:343–349
Landy A, Ross W (1977) Science 197:1147–1160
Lidholm J, Szmidt AE, Hallgren J-E, Gustafsson P (1988) Mol Gen Genet 212:6–10
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, NY
Milligan BG, Hampton JN, Palmer JD (1989) Mol Biol Evol 6:355–368
Ogihara Y, Terachi T, Sasakuma T (1988) Proc Natl Acad Sci USA 85:8573–8577
Palmer JD, Thompson WF (1982) Cell 29:537–550
Palmer JD (1985) Annu Rev Genet 19:325–354
Palmer JD (1986) Methods Enzymol 118:167–186
Palmer JD, Stein DB (1986) Curr Genet 11:823–834
Palmer JD, Osorio B, Aldrich J, Thompson WF (1987) Curr Genet 11:275–286
Pearson WR, Lipman DJ (1988) Proc Natl Acad Sci USA 85:2444–2448
Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467
Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H, Shugiura M (1986) EMBO J 5:2043–2049
Stein J vom, Hachtel W (1988) Mol Gen Genet 213:513–518
Strauss SH, Palmer JD, Howe GT, Doerksen AH (1988) Proc Natl Acad Sci USA 85:3898–3902
Tsai C-H (1989) Dispersed repetitive sequences in the chloroplast genome of Douglas-fir (Pseudotsuga menziesd (Mirb.) Franco). MS, thesis, Oregon State University, Corvallis, Ore
Wolfe KH, Li W-H, Sharp PM (1987) Proc Natl Acad Sci USA 84:9054–9058
Wolfe KH, Sharp PM (1988) Gene 66:215–222
Zhang H, Scholl R, Browse J, Somerville C (1988) Nucleic Acids Res 16:1220
Zhou DX, Massenet O, Quigley F, Marion MJ, Moneger F, Huber P, Mache R (1988) Curr Genet 13:433–439
Zurawski G, Clegg MT (1987) Annu Rev Plant Physiol 38:391–418
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Tsai, CH., Strauss, S.H. Dispersed repetitive sequences in the chloroplast genome of Douglas-fir. Curr Genet 16, 211–218 (1989). https://doi.org/10.1007/BF00391479
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DOI: https://doi.org/10.1007/BF00391479