Abstract
The bark of some young woody stems contains storage proteins which are subject to an annual rhythm: they accumulate in the autumn and are mobilized in the spring. We show here that the bark phoem-parenchyma cells of Sambucus nigra L. contain numerous protein bodies, and that the bark lectin (S. nigra agglutinin) which undergoes an annual rhythm is localized in these protein bodies. The protein bodies in the cotyledons of legume seeds also contain lectin, indicating that lectins may be storage compounds themselves or may have a function in storage and-or mobilization processes.
Similar content being viewed by others
Abbreviations
- PBS:
-
phosphate-buffered saline
- IgG:
-
immunoglobulin
- SNA:
-
Sambucus nigra agglutinin
References
Bollini, R., Chrispeels, M.J. (1978) characterization and subcellular localization of vicilin and phytohemagglutinin, the two major reserve proteins of Phaseolus vulgaris L. Planta 142, 291–298
Brandtzaeg, P. (1973) Conjugates of immunoglobulin G with different fluorochromes. I. Characterization by anionic-exchange chromatography. Scand. J. Immunol. 2, 273–290
Broekaert, W.F., Nsimba-Lubaki, M., Peeters, B., Peumans, W.J. (1984) A lectin from elder (Sambucus nigra L.) bark. Biochem. J. 221, 163–169
Chrispeels, M.J. (1984) Biosynthesis, processing and transport of storage proteins and lectins in cotyledons of developing legume seeds. Phil. Trans. R. Soc. London B 304, 309–322
Chrispeels, M.J., Baumgartner, B., Harris, N. (1976) Regulation of reserve protein metabolism in the cotyledons of mung bean seedlings. Proc. Natl. Acad. Sci. USA 73, 3168–3172
Hennerty, M.J., O'Kennedy, B.T., Titus, J.S. (1980) Conservation and reutilization of bark proteins in apple trees. In: Mineral nutrition of fruit trees, pp. 369–377, Atkinson, D., Jackson, J.E., Sharples, R.O., Waller, W.M., eds. Butterworths, London
Horisberger, M., Volanthen, M. (1980) Ultrastructural localization of soybean agglutinin on thin sections of Glycine max var. Altona, by the gold method. Histochemistry 65, 181–186
Kang, S.-M., Titus, J.S. (1980) Isolation and partial characterization of an acid endoprotease present in dormant apple shoot bark. Plant Physiol. 66, 984–989
Miller, J.B., Hsu, R., Heinrikson, R., Yachnin, S. (1975) Extensive homology between the subunits of phytohemagglutinin mitogenic proteins derived from Phaseolus vulgaris. Proc. Natl. Acad. Sci. USA 72, 1388–1391
Millerd, A. (1975) Biochemistry of legume seed proteins. Annu. Rev. Plant Physiol. 26, 53–72
O'Kennedy, B.T., Titus, J.S. (1979) Isolation and mobilization of storage proteins from apple shoot bark. Physiol. Plant. 45, 419–424
Titus, J.S., Kang, S-M. (1982) Nitrogen metabolism, translocation, and recycling in apple trees. Hort. Rev. 4, 204–246
Tokuyasu, K.T., Singer, S.J. (1976) Improved procedures for immunoferretin labeling of ultrathin frozen sections. J. Cell. Biol. 71, 894–906
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Greenwood, J.S., Stinissen, H.M., Peumans, W.J. et al. Sambucus nigra agglutinin is located in protein bodies in the phloem parenchyma of the bark. Planta 167, 275–278 (1986). https://doi.org/10.1007/BF00391426
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00391426