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Sambucus nigra agglutinin is located in protein bodies in the phloem parenchyma of the bark

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Abstract

The bark of some young woody stems contains storage proteins which are subject to an annual rhythm: they accumulate in the autumn and are mobilized in the spring. We show here that the bark phoem-parenchyma cells of Sambucus nigra L. contain numerous protein bodies, and that the bark lectin (S. nigra agglutinin) which undergoes an annual rhythm is localized in these protein bodies. The protein bodies in the cotyledons of legume seeds also contain lectin, indicating that lectins may be storage compounds themselves or may have a function in storage and-or mobilization processes.

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Abbreviations

PBS:

phosphate-buffered saline

IgG:

immunoglobulin

SNA:

Sambucus nigra agglutinin

References

  • Bollini, R., Chrispeels, M.J. (1978) characterization and subcellular localization of vicilin and phytohemagglutinin, the two major reserve proteins of Phaseolus vulgaris L. Planta 142, 291–298

    Google Scholar 

  • Brandtzaeg, P. (1973) Conjugates of immunoglobulin G with different fluorochromes. I. Characterization by anionic-exchange chromatography. Scand. J. Immunol. 2, 273–290

    Google Scholar 

  • Broekaert, W.F., Nsimba-Lubaki, M., Peeters, B., Peumans, W.J. (1984) A lectin from elder (Sambucus nigra L.) bark. Biochem. J. 221, 163–169

    Google Scholar 

  • Chrispeels, M.J. (1984) Biosynthesis, processing and transport of storage proteins and lectins in cotyledons of developing legume seeds. Phil. Trans. R. Soc. London B 304, 309–322

    Google Scholar 

  • Chrispeels, M.J., Baumgartner, B., Harris, N. (1976) Regulation of reserve protein metabolism in the cotyledons of mung bean seedlings. Proc. Natl. Acad. Sci. USA 73, 3168–3172

    Google Scholar 

  • Hennerty, M.J., O'Kennedy, B.T., Titus, J.S. (1980) Conservation and reutilization of bark proteins in apple trees. In: Mineral nutrition of fruit trees, pp. 369–377, Atkinson, D., Jackson, J.E., Sharples, R.O., Waller, W.M., eds. Butterworths, London

    Google Scholar 

  • Horisberger, M., Volanthen, M. (1980) Ultrastructural localization of soybean agglutinin on thin sections of Glycine max var. Altona, by the gold method. Histochemistry 65, 181–186

    Google Scholar 

  • Kang, S.-M., Titus, J.S. (1980) Isolation and partial characterization of an acid endoprotease present in dormant apple shoot bark. Plant Physiol. 66, 984–989

    Google Scholar 

  • Miller, J.B., Hsu, R., Heinrikson, R., Yachnin, S. (1975) Extensive homology between the subunits of phytohemagglutinin mitogenic proteins derived from Phaseolus vulgaris. Proc. Natl. Acad. Sci. USA 72, 1388–1391

    Google Scholar 

  • Millerd, A. (1975) Biochemistry of legume seed proteins. Annu. Rev. Plant Physiol. 26, 53–72

    Google Scholar 

  • O'Kennedy, B.T., Titus, J.S. (1979) Isolation and mobilization of storage proteins from apple shoot bark. Physiol. Plant. 45, 419–424

    Google Scholar 

  • Titus, J.S., Kang, S-M. (1982) Nitrogen metabolism, translocation, and recycling in apple trees. Hort. Rev. 4, 204–246

    Google Scholar 

  • Tokuyasu, K.T., Singer, S.J. (1976) Improved procedures for immunoferretin labeling of ultrathin frozen sections. J. Cell. Biol. 71, 894–906

    Google Scholar 

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Greenwood, J.S., Stinissen, H.M., Peumans, W.J. et al. Sambucus nigra agglutinin is located in protein bodies in the phloem parenchyma of the bark. Planta 167, 275–278 (1986). https://doi.org/10.1007/BF00391426

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  • DOI: https://doi.org/10.1007/BF00391426

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