Abstract
Photosynthesis, assimilate partitioning and intracellular distribution of orthophosphate (Pi) in barly (Hordeum vulgare L.) leaves were measured in plants grown with either 25, 1 or 0 mmol· 1−1 nutrient phosphate supply. Phosphate deficiency resulted in a significant decrease in the leaf Pi, diminished rates of photosynthesis and a decrease in the sucrose/starch ratio in the leaves. Changes in the cytoplasmic Pi content were relatively small in comparison with the large variations in vacuolar Pi. The cytoplasmic Pi concentration was slightly higher in the leaves of plants grown at 25 mmol·l−1 Pi than in those grown at 1 mmol·l−1 Pi and was decreased in the phosphate-deficient plants in which photosynthesis was inhibited. With barley plants grown in phosphate-deficient media, very little, if any, Pi was present in the vacuole. All of the cellular Pi was in the cytoplasm. Barley, spinach (Spinacia oleracea L.) and soya (Glycine max L.) plants were grown to a comparative stage of phosphate deficiency as measured by leaf Pi levels. These species showed a uniform response to phosphate deficiency by increasing starch synthesis relative to sucrose but the accompanying limitation on photosynthetic capacity varied considerably among the species. Interspecific differences in assimilate partitioning between starch and sucrose were maintained over a wide range of Pi supply.
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Abbreviations
- Hepes:
-
4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid
- 31P n.m.r.:
-
Phosphorus-31 nuclear magnetic resonance
- Pi:
-
orthophosphate
References
Arnon, D.I. (1949) Copper enzyme in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiol. 24, 1–15
Cockburn, W., Baldry, C.W., Walker D.A. (1967a) Oxygen evolution by isolated chloroplasts with carbon dioxide as the hydrogen acceptor. A requirement for orthophosphate or pyrophosphate. Biochim. Biophys. Acta 131, 594–596
Cockburn, W., Baldry, C.W., Walker, D.A. (1967b) Some effects of inorganic phosphate on O2 evolution by isolated chloroplasts. Biochim. Biophys. Acta. 143, 614–624
Doehlert, D.C., Huber, S.C. (1983) Spinach leaf sucrose phosphate synthetase activation by glucose-6-phosphate and inactivation with inorganic phosphate. FEBS Lett. 153, 293–298
Doehlert, D.C., Huber, S.C. (1984) Phosphate inhibition of spinach leaf sucrose phosphate synthetase as affected by glucose-6-phosphate and phosphoglucoisomerase. Plant Physiol. 76, 250–253
Edwards, G.E., Walker, D.A. (1983) C3,C4 Mechanisms: Cellular and environmental regulation of photosynthesis, pp. 498–500. Blackwell Scientific publications, Oxford London
Foyer, C., Walker, D., Spencer, C., Mann, B. (1982) Observations on the phosphate status and intracellular pH of intact cells, protoplasts and chloroplasts from photosynthetic tissue using phosphorus-31 nuclear magnetic resonance. Biochem. J. 202, 429–434
Furbank, R.T. Stitt, M., Foyer, C.H. (1985) Intercellular compartmentation of sucrose synthesis in leaves of Zea mays L. Planta 164, 172–178
Harbon, S., Foyer, C., Walker, D.A. (1981) The purification and properties of sucrose phosphate synthetase from spinach leaves: the involvement of this enzyme and fructose bisphosphatase in the regulation of sucrose biosynthesis. Arch. Biochem. Biophys. 212, 237–246
Herold, A. (1980) Regulation of photosynthesis by sink activity-the missing link. New Phytol. 86, 134–144
Heldt, H.W., Chon, C.J., Maronde, D., Herold, A., Stankovic, Z.S., Walker, D.A., Kraminer, A., Kirk, M.R., Heber, U. (1977) The role of orthophosphate and other factors in the regulation of starch formation in leaves and isolated chloroplasts. Plant Physiol. 59, 1146–1155
Heldt, H.W., Rapley, L. (1970) Specific transport of inorganic phosphate, 3-phosphoglycerate and dihydroxyacetonephosphate and of dicarboxylates across the inner membrane of spinach chloroplasts. FEBS Lett. 10, 143–148
Herzog, B., Stitt, M., Heldt, H.W. (1984) Control of photosynthetic sucrose synthesis by fructose 2,6-bisphosphate. Plant Physiol. 75, 561–565
Huber, S.C., (1981) Interspecific variation in activity and regulation of leaf sucrose phosphate synthase. Z. Pflanzenphysiol. 102, 443–450
Huber, S.C. (1983) Role of sucrose-phosphate synthase in partitioning of carbon in leaves. Plant Physiol. 71, 818–821
Jones, M.G.K., Outlaw, W.H., Lowry, O.H. (1977) Enzymic assay of 10−7 to 10−14 moles of sucrose in plant tissues. Plant Physiol. 60, 379–383
Mitsumori, F., Ito, O. (1984) Phosphorus-31 nuclear magnetic resonance studies of photosynthesising Chlorella. FEBS Lett. 174, 248–252
Portis, A.R. Jr. (1982) Effects of the relative extra-chloroplastic concentrations of inorganic phosphate, 3-phosphoglycerate and dihydroxyacetonephosphate on the rate of starch synthesis in isolated spinach chloroplasts. Plant Physiol. 70, 393–396
Rebeille, F., Bligny, R., Douce, R. (1982) Regulation of Pi uptake by Acer pseudoplatanus cells. Arch. Biochem. Biophys. 219, 371–378
Rebeille, F., Bligny, R., Martin, J-B., Douce, R. (1983) Relationship between the cytoplasm and the vacuole phosphate pool in Acer pseudoplatanus cells. Arch. Biochem. Biophys. 225, 143–148
Stitt, M., Herzog, R., Heldt, H.W. (1984a) Control of photosynthetic sucrose synthesis by fructose 2,6-bisphosphate. 1. Coordination of CO2 fixation and sucrose synthesis. Plant Physiol. 75, 548–553
Stitt, M., Kurzel, B., Heldt, H.W. (1984b) Control of photosynthetic sucrose synthesis by fructose 2,6-bisphosphate II. Partitioning between sucrose and starch. Plant Physiol. 75, 554–560
Stitt, M., Cseke, C., Buchanan, B.B. (1984c) Regulation of fructose 2,6-bisphosphate concentration in spinach leaves. Eur. J. Biochem. 143, 89–93
Stitt, M., Wirtz, W., Gerhardt, R., Heldt, H.W., Spencer, C., Walker, D., Foyer, C. (1985) A comparative study of metabolite levels in plant leaf material in the dark. Planta 166, 354–364
Taussky, H.H., Shorr, E. (1953) A microcolorimetric method for the determination of inorganic phosphorus. J. Biol. Chem. 202, 675–685
Waterton, J.C., Bridges, I.G., Irving, M.P. (1983) Intracellular compartmentation detected by 31P-NMR in intact photosynthetic wheat-leaf tissue. Biochim. Biophys. Acta 763, 315–320
Woodrow, I.E., Ellis, J.R., Jellings, A., Foyer, C.H. (1984) Compartmentation and fluxes of inorganic phosphate in photosynthetic cells. Planta 161, 525–530
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Foyer, C., Spencer, C. The relationship between phosphate status and photosynthesis in leaves. Planta 167, 369–375 (1986). https://doi.org/10.1007/BF00391341
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DOI: https://doi.org/10.1007/BF00391341