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Sequence and expression of endogenous S-locus glycoprotein genes in self-compatible Brassica napus

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Abstract.

Brassica napus is an amphidiploid plant which is self-compatible even though it is derived from hybridisation of the self-incompatible species B. oleracea and B. campestris. Experiments were undertaken to establish if S-locus glycoprotein (SLG) genes exist in B. napus and whether these are expressed as in self-incompatible Brassica species. Two different stigma-specific cDNA sequences homologous to SLG genes were obtained from the B. napus cultivar Westar. One of these sequences, SLG WS1, displayed highest homology to class I SLG alleles, whereas the other, SLG WS2, showed greatest homology to class II SLG genes. Both were expressed at high levels in Westar stigmas following a developmental pattern typical of SLG genes in the self-incompatible diploids. We infer that they represent the endogenous SLG genes at the two homoeologous S-loci. The occurrence of normally expressed SLG genes and its relevance to the self-compatible phenotype of B. napus is discussed.

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Author notes

  1. Sharon Allard

    Present address: Centre for Food and Animal Research, Central Experimental Farm, K1A 0C6, Ottawa, Ontario, Canada

Authors and Affiliations

  1. Plant Research Centre, Central Experimental Farm, K1A 0C6, Ottawa, Ontario, Canada

    Laurian S. Robert & Sharon Allard

  2. John Innes Centre, Cambridge Laboratory, Institute of Plant Science Research, NR4 7UJ, Norwich, UK

    Tanya M. Franklin & Martin Trick

Authors
  1. Laurian S. Robert
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  2. Sharon Allard
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  3. Tanya M. Franklin
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  4. Martin Trick
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Communicated by D.M. Lonsdale

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Robert, L.S., Allard, S., Franklin, T.M. et al. Sequence and expression of endogenous S-locus glycoprotein genes in self-compatible Brassica napus . Molec. Gen. Genet. 242, 209–216 (1994). https://doi.org/10.1007/BF00391015

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