Summary
In view of the great variation in the size of epithelial cell nucleic in benign changes in the mammary gland, the cytologist is often faced with diagnostic difficulties. Clear differences in the size of the nucleic in aspiration biopsies from the mammary glands of pregnant women and those from the mammary glands of post-menopausal women seem to indicate that even in normal, unchanged mammary gland epithelia, the cell nucleic size varies in accordance with the hormonal situation and any functional changes that might have occurred.
To test these findings, the nuclear area of the mammary gland epithelia was compared in four groups consisting of 20 post-menopausal women, 20 sexually mature women, 20 gravid women and 20 women taking ovulation inhibitors.
In addition, we measured the nuclear area of tumour cell nuclei from solidly growing mammary carcinomas and their hybrid forms. The 40 patients with mammary carcinomas included 20 sexually mature women and 20 post-menopausal women.
A comparison of the size of the nuclei of normal epithelial cells revealed distinct differences in each group. The only exceptions were the sexually mature women and the women taking ovulation inhibitors, between whom there was no significant difference. The average nuclear area in post-menopausal women was 48 μm2, in sexually mature women 75 μm2, in pregnant women 118 μm2, and in women taking ovulation inhibitors 79 μm2.
The difference between the size of the nucleic in the tumour cells and those in normal mammary gland epithelia was significant in all groups. The average size of the tumour cell nuclei in sexually mature women was 185 μm2, and in post-menopausal women 170 μm2. A major difference between the two tumour groups was the noticeably larger range in the size of the nuclear area in the sexually mature women.
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References
Bässler R, Forssmann W (1964) Experimenteller Strukturwandel der Drüsenzelle durch Hormonwirkung. Verh Dtsch Ges Pathol 48:240
Blume G (1970) Morphologische und histometrische Untersuchungen an der Brustdrüse der Ratte bei hormonaler Stimulation und Involution. Doctoral thesis, Mainz
Cornellisse, CJ, de Koning HR, Arentz PW, Raatgever JW, van Heerde P (1981) Quantitative analysis of the nuclear area variation in benign and malignant breast cytology specimens. Int Acad Cytol Analyt Quant Cytol 3:128–134
Cornillot M, Verhaeghe M (1959) Données cytologiques dans le ponctions des tumeurs du sein. Pathol Biol 7:793
Curtiss C (1949) Factors influencing lobulo-alveolar development and mammary secretion in the rat. Endocrinology 45:284
Kern W, Dermer G (1972) The cytopathology of hyperplastic and neoplastic mammary duct epithelium. Acta cytol 16:120
Kirkham WR, Turner W (1954) Introduction of mammary growth in rats by estrogen and progesteron. Proc Soc Exp Biol Med 87:139
Klein U (1971) Karyometrische und cytophotometrische Untersuchungen über den Kernstoffwechsel (DNS-PO4-Gruppen) der Milchdrüse bei hormonaler Stimulation. Doctoral thesis, Mainz
Koss L (1968) Diagnostic cytology and its histopathologic basis, 2nd edn. Lippincott, Philadelphia
Kreuzer G, Boquoi E (1981) Zytologie der weiblichen Brustdrüse. Thieme Stuttgart, New York
Leidenberger FA (1976) Hormonelle Regulation der Brustdrüsenfunktion beim Menschen. Fortschr Med 94:196
Murad TM, Synder ME (1973) The diagnosis of breast lesions from cytological material. Acta Cytol 17:418
Nienhaus H (1975) Karyometrische Befunde über Altersveränderungen der weiblichen Brustdrüse. Verh Dtsch Ges Pathol 59:422
Nienhaus H, Grundmann E (1980) Kanzerogenese der Mamma. MD-GBK 30 Bulletin of the GBK
Oehlert W, Schultze B (1960) Die Kerngröße als Ausdruck der synthetischen Aktivität des Kerns. Beitr Pathol Anat 123:101
Prechtel K (1970) Altersabhängiger Strukturwandel der weiblichen Brustdrüse. Verh Dtsch Ges Pathol 54:393
Schöndorf H (1977) Aspirationszytologie der Brustdrüse. Schattauer, Stuttgart, New York
Söderström N (1966) Fine-needle aspiration biopsy. Almquist and Wiksell, Stockholm, Göteborg, Uppsala
Tajima M, Tohma H, Inamura M, Nakamura N (1981) Studies of morphological characteristics of mammary aspiration smears. Nagoya J Med Sci 26:63–75
Takeda T (1977) Studies on cytological characteristics of mammary aspiration smears based on histological types. Acta Cytol 21:424
Zajdela A (1979) The relation of prognosis to the nuclear diameter of breast cancer cells obtained by cytologic aspiration. Acta Cytol 23:75
Zajick J (1974) Aspiration biopsy cytology, part I. Karger, Basel, New York
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Schöndorf, H., Naujoks, H. Determining the nuclear area in normal breast epithelia and in the nucleic of mammary carcinomas. J Cancer Res Clin Oncol 109, 241–244 (1985). https://doi.org/10.1007/BF00390363
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DOI: https://doi.org/10.1007/BF00390363