Summary
We have isolated 150 benomyl resistant mutants of the fission yeast Schizosaccharomyces pombe. Seven of these mutants were found to be cold sensitive for mitosis. These mutants were the subject of physiological, cytological and genetical characterisation. Growth and division of the seven mutants were similar to the wild type strain at 35 °C. After shift from the permissive (35 °C) to the restrictive temperature (20 °C) the mutants became blocked in mitosis whilst cellular growth continued. Consequently, elongate cells were formed. Six of the seven benomyl resistant mutants became blocked in mitosis at 20 °C with a single aberrant nucleus. In every case the benomyl resistant and cold sensitive phenotype was due to a mutation in a single nuclear gene. These mutants were found to comprise a single genetic linkage group (ben4) and were unlinked to existing TBZ/MBC resistant mutants of S. pombe. Whilst no cross resistance was found in our mutants to TBZ, six of the seven mutants were super sensitive to the spindle poison CIPC. We believe that the phenotype exhibited by these mutants is consistent with a defective tubulin subunit.
Similar content being viewed by others
References
Egel R (1973) Mol Gen Genet 121:277–284
Fantes PA (1979) Nature 279:428–430
Fantes PA (1982) J Cell Sci 55:383–402
Frankel J, Jenkins LM, DeBault LE (1976) J Cell Biol 71:242–260
Gutz H, Heslot H, Leupold U, Loprieno N (1974) Schizosaccharomyces pombe. In: King RC (ed) Handbook of Genetics vol 1. Plenum Press, New York, pp 395–445
Hartwell LH (1978) J Cell Biol 77:627–637
Howell S (1974) An analysis of cell cycle controls in temperature sensitive mutants of Chlamydomonas reinhardii. In: Padilla GM, Cameron IL, Zimmerman A (eds) Cell cycle controls. Academic press, New York, pp 235–249
Inoué S, Sato H (1967) J Gen Physiol 50:259–292
Jarvik J, Botstein D (1973) Proc Natl Acad Sci USA 70:2046–2050
King SM, Hyams J (1982) Protoplasma 110:54–62
Leupold U (1950) CR Lab Carlsberg Ser Physiol 24:381–480
McCully EK, Robinow CF (1971) J. Cell Sci. 9:475–507
Mitchison JM (1970) Physiological and cytological methods for S. pombe. In: Prescott DM (ed) Methods in cell physiology, vol. 4. Academic Press, New York, pp 131–165
Mitchison JM, Creanor J (1971) Expl Cell Res 67:368–374
Moir D, Stewart S, Botstein D (1978) Abstracts, 9th International Conference on Yeast Genetics and Molecular Biology, Rochester, New York, p 69
Morris NR (1980) Chromosome structure and the molecular biology of mitosis in eukaryotic micro-organisms. In Gooday GW, Lloyd D, Trinci APJ (eds) The Eukaryotic Microbial Cell. University Press, Cambridge, pp 41–76
Nasmyth K, Nurse P (1981) Mol Gen Genet 182:119–124
Neff N, Thomas JH, Botstein D (1981) In: Molecular Biology of Yeast. Cold Spring Harbour Symposium, p 246
Nurse P (1975) Nature 256:547–551
Nurse P (1981) Genetic Analysis of the Cell Cycle. In: Glover SW, Hopwood DA (eds) Genetics as a Tool in Microbiology. University Press, Cambridge, pp 291–315
Nurse P, Thuriaux P, Nasmyth K (1976) Mol Gen Genet 146:167–178
Oakley BR (1981) Mitotic Mutants. In: Cellular Dynamics: Mitosis/Cytokinesis. Academic Press, New York, pp 181–196
Peterson EL, Berger JD (1976) Can J Zool 54:2089–2097
Quinlan RA, Pogson CI, Gull K (1980) J Cell Sci 46:341–352
Roth LE (1967) J Cell Biol 34:47–59
Sheir-Neiss G, Lai MH, Morris NR (1978) Cell 15:638–647
Simchen G (1978) Ann Rev Genet 12:161–191
Thuriaux P, Nurse P, Carter B (1978) Mol Gen Genet 161:215–220
Toda T, Yamamoto M, Yanagida M (1981) J Cell Sci 52:271–287
Walker GM (1982) J Gen Microbiol 128:61–71
Weisenberg RC (1972) Science 177:1104–1105
Yamamoto M (1980) Mol Gen Genet 180:231–234
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Roy, D., Fantes, P.A. Benomyl resistant mutants of Schizosaccharomyces pombe cold-sensitive for mitosis. Curr Genet 6, 195–201 (1982). https://doi.org/10.1007/BF00390338
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00390338