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Nitrite reduction in leaves; Studies on isolated chloroplasts

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Summary

Chloroplast preparations from spinach leaves containing a high percentage of intact chloroplasts were capable of light dependent nitrite reduction at rates around 9 μmol/mg chlorophyll/h for at least 50 min. This reduction was inhibited by DCMU but unaffected by uncouplers of photosynthetic phosphorylation. Nitrite reduction was not accompanied by a stoichiometric evolution of oxygen evolution. The disappearance of nitrite was accompanied by an approximately stoichiometric formation of reduced nitrogen.

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Abbreviations

DCMU:

3-(3′,4′-dichlorophenyl)-1,1-dimethyl urea

CCCP:

carbonylcyanide m-chlorophenylhydrazone

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Author notes

  1. B. J. Miflin

    Present address: Biochemistry Department, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts

Authors and Affiliations

  1. Department of Plant Science, Faculty of Agriculture, The University, NE1 7RU, Neweastle upon Tyne, UK

    B. J. Miflin

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Miflin, B.J. Nitrite reduction in leaves; Studies on isolated chloroplasts. Planta 116, 187–196 (1974). https://doi.org/10.1007/BF00390226

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  • DOI: https://doi.org/10.1007/BF00390226

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