Summary
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1.
Like other photosynthesizing organisms which have been investigated, Ankistrodesmus braunii absorbs more glucose from the surrounding medium in the light than in the dark.
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2.
When the algae are incubated with glucose and 32P-labelled orthophosphate in short-time-experiments, the TCA-soluble organic phosphate fraction is markedly increased. No such effect is seen when 2-desoxy-glucose is administered to the algae instead of glucose.
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3.
In pre-labelled algae glucose causes an increase in the TCA-soluble acid-stable organic P-fraction which shows light-dependent saturation kinetics. In such experiments 2-desoxyglucose causes a linear increase in the acid-stable organic P-fraction which shows no light dependence.
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4.
DCMU and Antimycin A when given together block oxidative as well as light phosphorylation. These compounds do not, however inhibit the increase in the sugar-P-fraction caused by 2-desoxy-glucose in 32P-labelled algae.
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5.
The increase in the sugar-32P after administration of substrates to the algae is accompanied by a decrease in the fraction of the polyphosphates “C” and/or “D”.
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6.
These results are explained by assuming that an inorganic polyphosphate-glucose-phosphotransferase is active in Ankistrodesmus braunii.
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Herrn Prof. Harder zum 80. Geburtstag gewidmet
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Lysek, G., Simonis, W. Substrataufnahme und Phosphatstoffwechsel bei Ankistrodesmus braunii . Planta 79, 133–145 (1968). https://doi.org/10.1007/BF00390157
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DOI: https://doi.org/10.1007/BF00390157