Abstract
The uptake of sucrose against a concentration gradient into the dextran-impermeable [3H]H2O space of red beet (Beta vulgaris L.) vacuoles has been studied using silicone-layer-filtering centrifugation on both fluorometric and 14C-measurement of sucrose. Sucrose transport into vacuoles proceeds partly by an active transport system and partly by passive permeation. The K M(20°C) for active sucrose uptake was found to be about 22 mM and the V Max(20°C) was about 174 nmol sucrose x (unit betacyanin)-1 x h-1. The temperature dependency of sucrose transport appears to have an activation energy of 35,0 KJ×mol-1. Among various mono-, di-, and trisaccharides tested, raffinose acts as a competitive inhibitor of sucrose uptake.
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Abbreviations
- EDTA:
-
ethylenediamine tetraacetic acid
- fr. wt.:
-
fresh weight
- Tris:
-
tris-(hydroxymethyl)-aminomethan
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Willenbrink, J., Doll, S. Characteristics of the sucrose uptake system of vacuoles isolated from red beet tissue. Kinetics and specifity of the sucrose uptake system. Planta 147, 159–162 (1979). https://doi.org/10.1007/BF00389518
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DOI: https://doi.org/10.1007/BF00389518