Summary
In an effort to examine the specificity of the auxin transport system, the movement of a variety of growth substances and of auxin analogues through corn coleoptile sections was measured in both the basipetal and acropetal directions. In contrast to the basipetal, polar transport of the auxins indoleacetic acid (IAA) and 2,4-dichlorophenoxyacetic acid, no such movement was found for benzoic acid or for gibberellin A1. A comparison of the α- and β-isomers of naphthaleneacetic acid showed that the growth-active α-form is transported, but not the inactive β-analogue. Both the dextro (+) and leavo (-) isomer of 3-indole-2-methylacetic acid showed the basipetal movement characteristic of IAA, the dextro isomer being more readily transported than the (-)-form. In this instance, too, the transport was roughtly proportional to the growth promoting activity. The antiauxin p-chlorophenoxyisobutyric acid inhibited auxin transport as it inhibited auxin-induced growth. These results agree with the hypothesis that processes involved in auxin transport are closely linked to or even identical with the primary auxin action.
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References
Clor, M. A.: Translocation of tritium-labeled gibberellic acid in pea stem segments and potato tuber cylinders. Nature (Lond.) 214, 1263–1264 (1967).
Dörffling, K., u. M. Böttger: Transport von Abscisinsäure in Explantaten, Blattstiel- und Internodialsegmenten von Coleus rheneltianus. Planta (Berl.) 80, 299–308 (1968).
Evans, E. C., III, and B. E. Vaughan: Wounding response in relation to polar transport of radiocalcium in isolated root segments of Zea mays. Plant Physiol. 41, 1145–1151 (1966).
Evans, M. L., and P. M. Ray: Timing of the auxin response in coleoptiles and its implications regarding auxin action. J. gen. Physiol., 53, 1–20 (1969).
—, and R. Hokanson: Timing of the response of coleoptiles to application and withdrawal of various auxins. Planta (Berl.), 85, 85–95 (1969).
Foster, R. J., D. H. McRae, and J. Bonner: Auxin-antiauxin interaction at high auxin concentrations. Plant Physiol. 30, 323–327 (1955).
Fox, J. E., and J. S. Weis: Transport of the kinin, N6-benzyladenine: non-polar or polar. Nature (Lond.) 206, 678–679 (1965).
Gomori, G.: Preparation of buffers for use in enzyme studies. In: Methods of enzymology (S. P. Colowick and N. O. Kaplan, eds.), vol. I, p. 138–146. New York: Acad. Press 1955.
Hartt, C. E.: Light and translocation of C14 in detached blades of sugar cane. Plant Physiol. 40, 718–724 (1965).
Hertel, R., u. A. C. Leopold: Versuche zur Analyse des Auxintransports in der Koleoptile von Zea mays L. Planta (Berl.) 59, 535–562 (1963).
—, and R. Flory: Auxin movement in corn coleoptiles. Planta (Berl.) 82, 123–144 (1968).
Horton, R. F., and R. A. Fletcher: Transport of the auxin, pichloram, through petioles of bean and Coleus and stem sections of pea. Plant Physiol. 43, 2045–2048 (1968).
Jacobs, W. P.: Control of elongation in the bean hypocotyl by the ability of the hypocotyl tip to transport auxin. Amer. J. Bot. 37, 551–555 (1950).
—: Hormonal regulation of leaf abscission. Plant Physiol. 43, 1480–1495 (1968).
Jaffe, L. F.: Electrical currents through the developing Fucus egg. Proc. nat. Acad. Sci. (Wash.) 56, 1102–1109 (1966).
Joensson, A.: Chemical structure and growth activity of auxins and antiauxins. Handb. Pflanzenphysiol. (W. Ruhland, ed.), vol. XIV, p. 959–1006 Berlin-Göttingen-Heidelberg: Springer 1961.
Kaindl, K.: The action-concentration curves of mixtures of growth-promoting and growth-inhibiting substances. In: The chemistry and mode of action of plant growth substances (R. L. Wain and F. Wightman, eds.), p. 159–164. London: Butterworth 1956.
Keitt, G. W.: Auxin activity of substituted benzoic acids and their effect on polar auxin transport. Plant Physiol. 41, 1561–1569 (1966).
Kende, H. J.: Preparation of radioactive gibberellin A1 and its metabolism in dwarf peas. Plant Physiol. 42, 1612–1618 (1967).
Kutácek, M., et Z. Prochazka: Methodes de determination et d'isolement de composés indoliques chez les Crucifères. In: Régulateurs naturels de la croissance végétale, p. 445–455, Paris: C.N.R.S. 1964.
Leopold, A. C.: Plant Growth and Development, p. 116. New York: McGraw-Hill 1964.
—, and S. L. Lam: Polar transport of three auxins. In: Plant growth regulators (R. M. Klein, ed.), p. 411–418. Ames: Iowa State Univ. Press 1961.
McCready, C. C.: Movement of growth regulators in plants. I. Polar transport of 2,4-dichlorophenoxyacetic acid in segments from the petioles of Phaseolus vulgaris. New Phytologist 62, 3–18 (1963).
—: Translocation of growth regulators. Ann. Rev. Plant Physiol. 17, 283–294 (1966).
—. New Phytologist 62, 19–34 (1963).
Niedergang-Kamien, E., and A. C. Leopold: The inhibition of transport of indoleacetic acid by phenoxyacetic acids. Physiol. Plantarum (Copenh.) 12, 776–785 (1959).
Schlender, K. K.: Synthesis and physiological activity of several indole-3-alkanoic acids. Dissert. (M.S.), Michigan State University, East Lansing (1963).
Steeves, T. A., and W. R. Briggs: Morphogenetic studies on Osmunda cinnamonea L. J. exp. Bot. 11, 45–67 (1960).
Stowe, B. B., and J. F. Schilke: Submicrogram identification and analysis of indole auxin by gas chromatography and spectrophotofluorometry. In: Régulateurs naturels de la croissance végétale, p. 409–419. Paris: C.N.R.S. 1964.
Thimann, K. V., and W. D. Bonner Jr.: The action of tri-iodobenzoic acid on growth. Plant Physiol. 23, 158–161 (1948).
Udenfriend, S.: Fluorescence assay in biology and medicine. New York: Acad. Press 1962.
Veen, H.: On the relation between auxin transport and auxin metabolism in explants of Coleus. Planta (Berl.) 73, 281–295 (1967).
Zaerr, J. B., and J. W. Mitchell: Polar transport related to mobilization of plant constituents. Plant Physiol. 42, 863–874 (1967).
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Hertel, R., Evans, M.L., Leopold, A.C. et al. The specificity of the auxin transport system. Planta 85, 238–249 (1969). https://doi.org/10.1007/BF00389401
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DOI: https://doi.org/10.1007/BF00389401