Abstract
A strain of the marine hymenostome ciliate Uronema marinum was isolated in axenic culture. This strain, designated PW2, was cultivated in a chemically defined medium containing 17 amino acids, 4 nucleotides, 5 fatty acids, stigmasterol, 8 growth factors and an artificial sea water. Increased populations were observed when either phosphatidylinositol or Tween 80 were included in the medium. The vitamins thiamine, riboflavin, pyridoxal, nicotinamide, folic acid and pantothenate were required by U. marinum PW2. No requirement could be demonstrated for exogenous thioctic acid or biotin, although growth was diminished in their absence. The amino acid and nucleotide requirements were found to be higher than the levels reported for other ciliates. This strain was found to be euryhaline, with maximum growth observed in media of specific gravity 1.007, corresponding to approximately 25% sea water. Although no requirement could be demonstrated for Fe, Zn, Mn or Cu, high levels of these metals were observed to be toxic. Growth was observed over a pH range of 6.6 to 8.3, with an optimum observed near 7.3.
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Literature Cited
Allen, E.J.: On the culture of the plankton diatom Thalassiosira gravida Cleve, in artificial sea water. J. mar. biol. Ass. U.K. 10, 417–438 (1914)
Braarud, A.C.: Salinity as an ecological factor in marine phytoplankton. Pl. Physiol., Lancaster 4, 28–34 (1951)
Conner, R.L., J.R. Landrey, E.S. Kaneshiro and W.J. van Wagtendonk: The metabolism of stigmasterol and cholesterol by Paramecium aurelia. Biochim. biophys. Acta 239, 312–319 (1971)
Dewey, V.C. and G.W. Kidder: Amino acid antagonisms in Tetrahymena. Archs Biochem. Biophys. 73, 29–37 (1958)
Dickerson, B., E.G. Fischer and D.M. Lilly: Axenic cultures of Paramecium calkinsi for osmotic experiments. Am. Zool. 3, p. 281 (1964)
Gold, K.: Methods for growing tintinnida in continuous culture. Am. Zool. 13, 203–208 (1973)
Gubler, C.J. and G.E. Bethsold: Studies on the physiological effects of thiamine. II. Effects of sorbitol on growth and α-ketoacid metabolism in thiamine-deficient and antagonist-treated rats. J. Nutr. 77, 332–342 (1962)
Hall, R.P.: Nutrition and growth of protozoa. In: Research in protozoology, Vol. I. pp 337–404. Ed. by T.T. Chen. Oxford: Pergamon Press Ltd. 1967
Hanna, B.A. and D.M. Lilly: Axenic cultivation of Uronema marinum. Am. Zool. 10, 539–540 (1970)
——: Nutrition of axenic Uronema marinum. Am. Zool. 11, p. 640 (1971)
——: Nutritional varieties of Uronema marinum. J. Protozool. 19 (Suppl.), p. 37 (1972)
Hill, D.L.: The biochemistry and physiology of Tetrahymena, 320 pp. New York: Academic Press 1972
Holz, G.G. Jr., J. Erwin and B. Wagner: The sterol requirements of Tetrahymena paravorax RP. J. Protozool. 8, 297–300 (1961a)
—, B. Wagner, J. Erwin, J.J. Britt and K. Bloch: Sterol requirements of a ciliate, Tetrahymena corlissi Th-X. Comp. Biochem. Physiol. 2, 202–217 (1961b)
—— and N. Rosenbaum: The nutritional of Tetrahymena setifera HZ-1; sterol and alcohol requirements. J. Protozool. 9, 359–363 (1962)
Kidder, G.W. and V.C. Dewey: Studies on the biochemistry of Tetrahymena. XII. B-vitamin requirements. Archs Biochem. 21, 66–72 (1949)
——: biochemistry of ciliates in pure culture. In: Biochemistry and physiology of Protozoa, Vol. I. pp 323–400. Ed. by A. Lwoff. New York: Academic Press 1951
Lee, J.J., J.H. Tietjen and C. Mastropolo: Axenic culture of the marine hymenostome ciliate Uronema marinum in chemically defined medium. J. Protozool. 18 (Suppl.), p. 10 (1971)
Lilly, D.M.: Growth factors in protozoa. In: Chemical zoology, Vol. I. pp 275–309. Ed. by G.W. Kidder. New York: Academic Press 1967
— and R. Stillwell: Probiotics: growth promoting factors produced by microorganisms. Science, N.Y. 147, 747–748 (1965)
Lwoff, A.: Sur la nutrition des infusorie. C.r. hebd. Séanc. Acad. Sci., Paris 176, 928–931 (1923)
Meskill, V.P.: Factors influencing the growth of Glaucoma chattoni in chemically defined medium. J. Protozool. 17, 104–107 (1970)
Morgan, T.B. and J. Yudkin: The vitamin sparing action of sorbitol. Nature, Lond. 180, 543–545 (1957)
Napolitano, R.L. and D.M. Lilly: Tolerance of Paramecium sp. for estuarine conditions. J. Protozool. 19 (Suppl.), p. 33 (1972)
Provasoli, L., J.J.A. McLaughlin and M.R. Droop: The development of artificial media for marine algae. Arch. Mikrobiol. 25, 392–428 (1957)
—— and I.J. Pinter: Relative and limiting concentrations of major mineral constituents for the growth of algal flagellates. Trans. N.Y. Acad. Sci. 16, 412–417 (1954)
Soldo, A.T. and E. Merlin: The cultivation of marine ciliates in axenic medium. J. Protozool. 18 (Suppl.), p. 26 (1971)
——: The cultivation of symbiont-free marine ciliates in axenic medium. J. Protozool. 19, 519–524 (1972)
— and E. Merlin and W.J. van Wagtendonk: The nutrition of Paramecium aurelia stock 299. J. Protozool. 16, 500–506 (1969)
Thompson, J.C. Jr.: A redescription of Uronema marinum, and a proposed new family Uronematidae. Va J. Sci. 15, 80–87 (1964)
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Communicated by J. Bunt, Miami
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Hanna, B.A., Lilly, D.M. Growth of Uronema marinum in chemically defined medium. Marine Biology 26, 153–160 (1974). https://doi.org/10.1007/BF00388884
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DOI: https://doi.org/10.1007/BF00388884