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Time course of hormonal control of the first mitosis in tobacco mesophyll protoplasts cultivated in vitro

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Abstract

The presence of auxin and cytokinin is necessary for the induction of mitosis in tobacco mesophyll protoplasts cultivated in vitro. In their absence, protoplasts firstly accumulate inhibitors of mitosis in the culture medium, possibly because of non-coordinated cell-wall synthesis, and secondly evolve a nonmitotic and degenerative metabolism. By changing the intoxified medium, it is possible to show that auxin is necessary from the beginning of culture, while cytokinin is only required later to allow a step in the development of the mitotic apparatus.

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Abbreviations

2.4-D:

2.4 dichlorophenoxyacetic acid

6-BA:

6-benzyladenine

NAA:

naphthaleneacetic acid

IAA:

indoleacetic acid

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Authors and Affiliations

  1. Laboratoire de Physiologie Végétale, Université de Perpignan, F-66025, Perpignan-Cedex, France

    Yves Meyer & Richard Cooke

Authors
  1. Yves Meyer
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  2. Richard Cooke
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Meyer, Y., Cooke, R. Time course of hormonal control of the first mitosis in tobacco mesophyll protoplasts cultivated in vitro. Planta 147, 181–185 (1979). https://doi.org/10.1007/BF00388735

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  • DOI: https://doi.org/10.1007/BF00388735

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