Summary
Two celllines from Daucus carota callus were tested for phenylalanine ammonia-lyase activity. The white callus (DCw) has only 15% of the enzyme activity of the blue callus (DCb). The enzyme activity reaches a maximum value after a cultivation period of 21 days, whereas the maximum in anthocyanin content is reached after 28 days. We can demonstrate here a good correlation between one of the enzymes and the end product of the anthocyanin biosynthesis.
The crude extract from DCw cells has no inhibitory effect on the enzyme from DCb-cells. Gibberellic acid A3, a component of the culture medium for DCw cells, does not act as an inhibitor on the partially purified enzyme. The procedure of purification and the characteristics of phenylalanine ammonia-lyase from the callus of Daucus carota are described.
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Literatur
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Heinzmann, U., Seitz, U. Beziehung von Anthocyansynthese und Enzymaktivität der Phenylalanin-Ammonium-Lyase (PAL) bei Kalluskulturen von Daucus carota . Planta 117, 75–81 (1974). https://doi.org/10.1007/BF00388680
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DOI: https://doi.org/10.1007/BF00388680