Summary
The dependence of the ATP level of the blue-green alga Anacystis nidulans on light intensity (300–20000 erg·cm-2·s-1 white light) and on temperature (0–50°), pH (6.5–9.0), and phosphate concentration (up to 10-1 M) in light (20000 lx) and dark was measured. In contrast to a low ATP level in darkness the level in light remains high and constant with each treatment in a broad range of the respective scales. DCMU [3-(3′,4′-dichlorophenyl)-l,1-dimethyl-urea)] (5×10-6 M) has no effect on the ATP pool in light, supporting the view that the ATP level is maintained by cyclic photophosphorylation. Dio-9, an inhibitor of energy transfer, lowers the ATP level according to concentration in light and dark. The unchanged ATP level after transitions from N2 to N2+CO2 under different conditions indicates an intensive regulation of the ATP pool. The ADP level, where measured, was found to be inverse to the ATP level. These results are discussed in comparison with data collected by other authors using different organisms.
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Abbreviations
- DCMU:
-
3-(3′,4′-dichlorophenyl)-1,1-dimethylurea
References
Biggins, J.: Respiration in blue-green algae. J. Bact. 99, 570–575 (1969)
Bornefeld, T., Domanski, J., Simonis, W.: Influence of light conditions, gassing and inhibitors on photophosphorylation and ATP level in Anacystis nidulans. In: Proc. II. Intern. Congr. on Photosynth., vol. II, p. 1379–1386, Forti, G., Avron, M., Melandri, A., eds. The Hague: Dr. W. Junk N. V. Publishers 1972
Eßl, A.: Vergleichende Untersuchungen über die Wirkungsspektren der Photophosphorylierungsprozesse und der photosynthetischen Sauerstoffproduktion bei der Blaualge Anacystis nidulans. Dissertation Würzburg 1969
Forti, G., Parisi, B.: Evidence for the occurence of cyclic photophosphorylation in vivo. Biochim. biophys. Acta (Amst.) 71, 1–6 (1963)
Heldt, H. W., Sauer, F., Rapley, L.: Differentiation of the permeability properties of the two membranes of the chloroplast envelope. In: Proc. II. Intern. Congr. Photosynth. Res., vol. II, p. 1345–1355; Forti, G., Avron, M., Melandri, A., eds. The Hague: Dr. W. Junk N. V. Publishers 1972
Jaworek, D., Gruber, W., Bergmeyer, H. U.: Adenosin-5′-diphosphat und Adenosin-5′-monophosphat. In: Methoden der enzymatischen Analyse, 2nd edn., vol. II, p. 2051–2053, Bergmeyer, H. U., ed. Weinheim: Verlag Chemie 1970
Jeschke, W.-D.: Über den lichtgeförderten Influx von Ionen in Blättern von Elodea densa. Vergleich der Influxe von K+- und Cl--Ionen. Planta (Berl.) 103, 164–180 (1972)
Jones, P. C. T.: The effect of light, temperature, and anaestetics on ATP levels in the leaves of Chenopodium rubrum and Phaseolus vulgaris. J. exp. Bot. 21, 58–63 (1970)
Kratz, W. A., Myers, J.: Photosynthesis and respiration of three blue-green algae. Plant Physiol. 30, 275–280 (1955)
Lewenstein, A., Bachofen, R.: Transient induced oscillations in the level of ATP in Chlorella fusca. Biochim. biophys. Acta (Amst.) 267, 80–85 (1972)
Santarius, K. A., Heber, U.: Changes in the intracellular levels of adenosine 5′-triphosphate, adenosine 5′-diphosphate, adenosine 5′-monophosphate and inorganic phosphate and regulatory function of the adenylate system in leaf cells during photosynthesis. Biochim. biophys. Acta (Amst.) 102, 39–54 (1965)
Simonis, W., Urbach, W.: Photophosphorylation in vivo. Ann. Rev. Plant Physiol. 24, 89–114 (1973)
Trebst, A., Pistorius, E.: ATP formation in photosynthetic NADP+ reduction with artificial electron donors in isolated chloroplasts. In: Progr. Photosynth. Res., vol. III, p. 1229–1235, Metzner, H., ed. Tübingen 1969
Ullrich, W. R.: Nitratabhängige nichtcyclische Photophosphorylierung bei Ankistrodesmus braunii in Abwesenheit von CO2 und O2. Planta (Berl.) 100, 18–30 (1971)
Ullrich-Eberius, C., Simonis, W.: Der Einfluß von Natrium- und Kaliumionen auf die Phosphataufnahme bei Ankistrodesmus braunii. Planta (Berl.) 93, 214–226 (1970)
Urbach, W., Kaiser, W.: Changes of ATP levels in green algae and intact chloroplasts by different photosynthetic reactions. In: Proc. II. Intern. Congr. Photosynth. Res., vol. II, p. 1401–1411, Forti, G., Avron, M., Melandri, A., eds. The Hague: Dr. W. Junk N. V. Publishers 1972
Welsch, F., Smith, L.: Kinetics of synthesis and utilization of adenosine triphosphate by intact cells of Rhodospirillum rubrum. Biochemistry 8, 3403–3408 (1969)
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Bornefeld, T., Simonis, W. Effects of light, temperature, pH, and inhibitors on the ATP level of the blue-green alga Anacystic nidulans . Planta 115, 309–318 (1974). https://doi.org/10.1007/BF00388613
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DOI: https://doi.org/10.1007/BF00388613