Abstracts
The interaction between histamine, adenosine triphosphate (ATP) and noradrenaline was studied with the perforated-patch technique in single cells isolated from the rabbit pulmonary artery. In these cells all of the agents activated caffeine-sensitive currents. In potassium-free conditions at a holding potential of −50 mV bath applied histamine, in concentrations that did not produce a response, greatly enhanced the magnitude of inward currents evoked by ionophoretic application of noradrenaline. These inward currents were calcium-activated chloride currents, I Cl(Ca). In potassiumcontaining solutions at a holding potential of 0 mV, bath applied histamine potentiated the outward currents (calcium-activated potassium currents (I K(Ca)) induced by noradrenaline. This synergistic action was rapid in onset, sustained during the continued presence of histamine and reversible. Bath application of noradrenaline inhibited the response to ionophoretically applied noradrenaline but not the caffeine-induced currents. ATP also stimulated I Cl(Ca) and I K(Ca) through a mechanism dependent on the caffeine-sensitive intracellular calcium store and also potentiated the currents activated by noradrenaline. It is concluded that one explanation for the phenomenon of potentiation in smooth muscle is convergence of several distinct pharmacological receptors to a common cellular mechanism.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aickin CC (1990) Chloride transport across the sarcolemma of vertebrate smooth and skeletal muscle. In: Alvarez-Leefmans FJ, Russell JM (eds) chloride channels and carriers in nerve, muscle and glial cells. Plenum Press, New York
Benham CD, Bolton TB, Byrne NG, Large WA (1987) Action of externally applied adenosine triphosphate on single smoothmuscle cells dispersed from the rabbit ear artery. J Physiol (Lond) 387:473–488
Bevan JA, Duckles SP, Lee JF (1975) Histamine potentiation of nerve- and drug-induced responses of a rabbit cerebral artery. Circ Res 36:647–653
Bolton TB, Large WA (1986) Are junction potentials essential? Dual mechanism of smooth-muscle cell activation by transmitter released from autonomic nerves. Q J Exp Physiol 71:1–28
De La Lande IS, Cannell VA, Waterson JG (1966) The interaction of serotonin and noradrenaline on the perfused artery. Br J Pharmacol 28:255–272
Droogmans G, Callewaert G, Declerck I, Casteels R (1991) ATP-induced Ca2+ release and Cl− current in cultured smoothmuscle cells from pig aorta. J Physiol (Lond) 440:623–634
Foldes A, Hall RC (1979) The effects of histamine on responses of the rabbit ear artery to electrical stimulation and to exogenous noradrenaline. Br J Pharmacol 67:329–335
Friel DD (1988) An ATP-sensitive conductance in single smooth-muscle cells from the rat vas deferens. J Physiol (Lond) 401:361–380
McGrath MA, Shepherd JT (1976) Inhibition of adrenergic neurotransmission in canine vascular smooth muscle by histamine. Circ Res 39:566–573
Schneider P, Hopp HH, Isenberg G (1991) Ca2+ influx through ATP-gated channels increments [Ca2+]i and inactivates ICa in myocytes from guinea-pig urinary bladder. J Physiol (Lond) 440:479–496
Suzuki H, Kou K (1983) Direct and indirect effects of histamine on the smooth muscle cells of the guinea-pig main pulmonary artery. Pflügers Arch 399:46–53
Van Breemen C, Saida K (1989) Cellular mechanisms regulating [Ca2+]i smooth muscle. Ann Rev Physiol 51:315–329
Wang Q, Large WA (1993) Action of histamine on single smooth muscle cells dispersed from the rabbit pulmonary artery. J Physiol (Lond) 468:125–139
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Helliwell, R.M., Wang, Q., Hogg, R.C. et al. Synergistic action of histamine and adenosine triphosphate on the response to noradrenaline in rabbit pulmonary artery smooth muscle cells. Pflugers Arch. 426, 433–439 (1994). https://doi.org/10.1007/BF00388307
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00388307