Summary
The amino acid permeability of the envelope of intact, functional spinach (Spinacia oleracea L.) chloroplasts was investigated by light scattering, volumetry and uptake of 14C-labelled amino acids. The criterion for the functionally of the chloroplasts was their ability to reduce CO2, PGA and oxaloacetate in the light at high rates.
Net uptake into the chloroplast interior of neutral amino acids such as alanine, glycine, serine, proline, threonine or valine occurred only at very low rates. The uptake was concentration dependent, indicating unspecific diffusion rather than carrier-mediated transport. The slowness of uptake is emphasized by the capability of neutral amino acids to provide osmotic support for intact chloroplasts during a considerable length of time. Back-exchange experiments also failed to indicate the existence of specific exchange carriers for the transport of neutral amino acids such as alanine or glycine through the envelope of intact chloroplasts. Dicarboxylic amino-acids are known to be taken up by the so-called dicarboxylate translocator. The same carrier was found to catalyze also the transfer of asparagine and glutamine.
The data do not support current assumptions concerning fast carrier-mediated transport of neutral amino acids and their role in the transfer of carbon during photosynthesis.
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References
Aach, H. G., Heber, U.: Kompartimentierung von Aminosäuren in der Blattzelle. Planta (Berl.) 57, 317–328 (1967)
Crofts, A. R., Deamer, D. W., Packer, L.: Mechanism of light-induced structural changes in chloroplasts. II. The role of ion movements in volume changes. Biochim. biophys. Acta (Amst.) 131, 97–118 (1967)
Chappel, J. B., Crofts, A. R.: Ion transport and reversible volume changes of isolated mitochondria. In: Regulation of Metabolic Processes in Mitochondria, p. 293–316, (Jager, J. M., Papa, S., Quagliariello, E., Slater, E. C., eds.) Amsterdam: Elsevier 1966
Hall, D. O.: Nomenclature for isolated chloroplasts. Nature (Lond.) New Biol. 235, 125–126 (1972)
Heber, U.: Protein synthesis in chloroplasts during photosynthesis. Nature (Lond.) 195, 91–92 (1962)
Heber, U.: Stochiometry of reduction and phosphorylation during illumination of intact chloroplasts. Biochim. biophys. Acta (Amst.) 305, 140–152 (1973)
Heber, U.: Metabolite exchange between chloroplasts and cytoplasm. Ann. Rev. Plant Physiol. 25, 28–55 (1974)
Heber, U., Sartorius, K. A.: Compartmentation and reduction of pyridine nucleotides in relation to photosynthesis. Biochim. biophys Acta (Amst.) 109, 390–498 (1065)
Heber, U., Santarius, K. A.: Direct and indirect transfer of ATP and ADP across the chloroplast envelope. Z. Naturforsch. 25b, 718–728 (1970)
Heldt, H. W.: Adenine nucleotide translocation in spinach chloroplasts. FEBS Letters 5, 11–14 (1969)
Heldt, H. W., Rapley, L.: Specific transport of inorganic phosphate, 3-phosphoglycerate and dihydroxyacetonephosphate, and of dicarboxylates across the inner membrane of spinach chloroplasts. FEBS Letters 10, 143–148 (1970)
Heldt, H. W., Sauer, F.: The inner membrane of the chloroplast envelope as the site of specific metabolite transport. Biochim. biophys. Acta (Amst.) 234, 83–91 (1971)
Hellbust, J. A., Bidwell, R. G. S.: Protein turnover in attached wheat and tobacco leaves. Canad. J. Bot. 42, 1–12 (1964)
Jacobs, M. H.: Some aspects of cell permeability to weak electrolytes. Cold Spr. Harb. Symp. Quant. Biol. 8, 30–38 (1940)
Jensen, R. G., Bassham, J. A.: Photosynthesis by isolated chloroplasts. Proc. nat. Acad. Sci. (Wash.) 56, 1095–1101 (1966)
Kirk, P. R., Leech, R. M.: Amino acid biosynthesis during photosynthesis by isolated chloroplasts. Plant Physiol. 50, 228–234 (1972)
Klingenberg, M., Pfaff, E.: Means of terminating reactions. In: Methods of Enzymology, vol. X, p. 680–684, Colowick, S. P., Kaplan, N. I., eds. New York: Acad. Press 1967
Nobel, P. S., Cheung, Y. S.: Two amino acid carriers in pea chloroplasts. Nature (Lond.) New Biol. 237, 207–208 (1972)
Nobel, P. S., Wang, C. T.: Amino acid permeability of pea chloroplasts as measured by osmotically determined reflection coefficients. Biochim. biophys. Acta (Amst.) 211, 78–87 (1970)
Ongun, A., Stocking, C. R.: Effects of light and dark on the intracellular fate of photosynthetic products. Plant Physiol. 40, 825–831 (1965)
Packer, L., Siegenthaler, P. A., Nobel, P. S.: Light-induced volume changes in spinach chloroplasts. J. Cell Biol. 26, 593–599 (1965)
Parthier, B.: Proteinsynthese in grünen Blättern. I. Die Proteinsynthese in verschiedenen subzellulären Fraktionen nach 14CO2-Photosynthese. Z. Naturforsch. 19b, 235–248 (1964)
Roberts, G. R., Keys, A. J., Whittingham, C. P.: The transport of photosynthetic products from the chloroplasts of tobacco leaves. J. exp. Bot. 21, 683–692 (1970)
Stein, W. D.: The movements of molecules across the cell membrane. New York: Acad. Press 1967
Wang, C. T., Nobel, P. S.: Permeability of pea chloroplasts to alcohols and aldoses as measured by reflection coefficients. Biochim. biophys. Acta (Amst.) 241, 200–212 (1971)
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Dedicated to Professor W. Simonis on the occasion of his 65th birthday
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Gimmler, H., Schäfer, G., Kraminer, H. et al. Amino acid permeability of the chloroplast envelope as measured by light scattering, volumetry and amino acid uptake. Planta 120, 47–61 (1974). https://doi.org/10.1007/BF00388270
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DOI: https://doi.org/10.1007/BF00388270