Summary
The time courses of the level of ascorbate oxidase (AO; EC 1.10.3.3.) were followed in the different organs (cotyledons, hypocotyl, taproot) of the developing mustard seedling. Phytochrome (operationally, far-red light, cf. [20]) rapidly and strongly enhances the rate of apparent ascorbate oxidase (AO)1 synthesis in cotyledons and hypocotyl, while in the taproot the detectable amount of AO is only small. However, the relative increase of AO as mediated by continuous far-red light is the same in all organs. Far-red → dark kinetics indicate that the phytochrome-induced enzyme is much less stable in the hypocotyl than in the cotyledons, at least during the experimental period. It is concluded that the effect of phytochrome on enzyme induction is precisely the same in cotyledons and hypocotyl, while the processes of enzyme degradation are specific for the organ. Thus the time courses of enzyme levels can be determined by the “nature” of the particular organ, even if no isoenzymes are involved and the “mechanism” of the inductive process is the same in the different organs.
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References
Beevers, H.: Respiratory metabolism in plants. Evanston, Ill.-White Plains, New York: Row, Peterson and Company 1961.
Dittes, L., Rissland, I., Mohr, H.: On the regulation of enzyme levels (phenylalanine ammonia-lyase) in different organs of a plant (Sinapis alba L.). Z. Naturforsch. 26b, 1175–1180 (1971).
Drumm, H., Elchinger, I., Möller, J., Peter, K., Mohr, H.: Induction of amylase in mustard seedlings by phytochrome. Planta (Berl.) 99, 265–274 (1971).
Häcker, M.: Der Abbau von Speicherprotein und die Bildung von Plastiden in den Kotyledonen des Senfkeimlings (Sinapis alba L.) unter dem Einfluß des Phytochroms. Planta (Berl.) 76, 309–325 (1967)
Hallaway, M., Phethean, P. D., Taggart, J.: A critical study on the intracellular distribution of ascorbate oxidase and a comparison of the kinetics of the soluble and cell-wall enzyme. Phytochem. 9, 935–944 (1970).
Hanke, J., Hartmann, K. M., Mohr, H.: Die Wirkung von “Störlicht” auf die Blütenbildung von Sinapis alba L., Planta (Berl.) 86, 235–249 (1969).
Hartmann, K. M.: A general hypothesis to interpret high energy phenomena of photomorphogenesis on the basis of phytochrome. Photochem. Photobiol. 5, 349–366 (1966).
Lange, H., Bienger, I., Mohr, H.: Eine neue Beweisführung für die Hypothese einer differentiellen Genaktivierung durch Phytochrom 730. Planta (Berl.) 76, 359–366 (1967).
Lange, H., Shropshire, W., Mohr, H.: An analysis of phytochrome-mediated antocyanin synthesis. Plant Physiol. 47, 649–655 (1971).
Mapson, L. W.: Metabolism of ascorbic acid in plants: part I. function. Ann. Rev. Plant Physiol. 9, 119–150 (1958).
Masoner, M., Unser, G., Mohr, H.: Accumulation of protoclorophyll and chlorophyll a as controlled by photomorphogenically effective light. Planta (Berl.) (in press).
Möller, J., Poucke, M. van: Gel electrophoretic comparison of light-induced ascorbic acid oxidase from mustard seedlings and from pumpkin tissue. Phytochem. 9, 1803–1805 (1970).
Mohr, H.: Untersuchungen zur phytochrominduzierten Photomorphogenese des Senfkeimlings (Sinapis alba L.). Z. Pflanzenphysiol. 54, 63–83 (1966).
Mohr, H.: Regulation der Enzymsynthese bei der höheren Pflanze. Naturwiss. Rdsch. 23, 187–195 (1970).
Mohr, H.: Lectures on photomorphogenesis. Berlin-Heidelberg-New York: Springer (in press).
Mohr, H., Bienger, I., Lange, H.: Primary reaction of phytochrome. Nature (Lond.) 230, 56–58 (1971).
Mohr, H., Meyer, U., Hartmann, K.: Die Beeinflussung der Farnsporen-Keimung [Osmunda cinnamomea (L.) und O. claytoniana (L.)] über das Phytochromsystem und die Photosynthese. Planta (Berl.) 60, 483–496 (1964).
Oberbacher, M. F., Vines, H. M.: Spectrophotometric assay of ascorbic acid oxidase. Nature (Lond.) 197, 1203–1204 (1963).
Schopfer, P., Hock, B.: Nachweis der phytochrom-induzierten de-novo-Synthese von Phenylalaninammoniumlyase (PAL, EC 4.3.1.5.) in Keimlingen von Sinapis alba L. durch Dichtemarkierung mit Deuterium. Planta (Berl.) 96, 248–253 (1971).
Schopfer, P., Mohr, H.: Phytochrome mediated induction of phenylalanine ammonia-lyase in mustard seedlings: a contribution to eliminate some misconceptions. Plant. Physiol. 49, 8–10 (1972).
van Poucke, M., Barthe, F., Mohr, H.: Phytochrome-mediated induction of ascorbic acid oxidase in mustard seedlings. Naturwissenschaften 56, 417 (1969).
van Poucke, M., Barthe, F.: Induction of glycolic acid oxidase activity in mustard seedlings under the influence of continuous irradiation with red and far-red light. Planta (Berl.) 94, 308–318 (1970).
Weidner, M.: Der DNS-Gehalt von Kotyledonen und Hypocotyl des Senfkeimlings (Sinapis alba L.) bei der phytochromgesteuerten Photomorphogenese. Planta (Berl.) 75, 94–98 (1967).
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Drumm, H., Brüning, K. & Mohr, H. Phytochrome-mediated induction of ascorbate oxidase in different organs of a dicotyledonous seedling (Sinapis alba L.). Planta 106, 259–267 (1972). https://doi.org/10.1007/BF00388103
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DOI: https://doi.org/10.1007/BF00388103