Abstract
The ultrastructure and distribution of the Golgi apparatus in developing wheat endosperm was investigated using a zinc iodide-osmium tetroxide staining complex in conjunction with low and high voltage electron microscopy. Dictyosomes were numerous in starchy endosperm and aleurone at 15 days after anthesis, and during the period of rapid storage protein deposition 25 d after anthesis. Fewer dictyosomes were seen in maturing endosperm. Two types of vesicles were associated with the dictyosomes; small, heavily-stained vesicles were sited at the ends of fine tubules which extend from the cisternae, and larger less-stained vesicles were associated with the periphery of the cisternae. Stereo-pairs of micrographs up to 1 μm thick were taken to demonstrate the interconnections between cisternal and tubular endoplasmic reticulum. Elements of tubular ER were closely associated with dictyosomes, but connections were not observed. These results are discussed in relation to the transport of endosperm storage proteins from their site of synthesis on the cisternal ER to their site of storage, the protein bodies.
Similar content being viewed by others
References
Barlow, K.K., Lee, J.W., Vesk, M. (1974) Morphological development of storage protein bodies in wheat. In: Mechanisms of regulation of plant growth, pp. 793–797, Bieleski, R.L., Ferguson, A.R., Creswell, M.M., eds., Bull. No. 12, Roy. Soc. of New Zealand, Wellington
Briarty, L.G. (1978) The mechanisms of protein body deposition in legumes and cereals. In: Plant proteins, pp. 81–106, Norton, G., ed. Butterworth, London Boston
Briarty, L.G., Hughes, C.E., Evers, A.D., (1979) The developing endosperm of wheat — a stereological analysis. Ann. Bot. 44, 641–658
Buttrose, M.S. (1963) Ultrastructure of the developing wheat endosperm. Aust. J. Biol. Sci. 16, 305–317
Campbell, W.P., Lee, J.W., O'Brien, T.P., Smart, M.G. (1981) Endosperm morphology and protein body formation in developing wheat grain. Aust. J. Plant Physiol. 8, 5–19
Harris, N. (1978) Nuclear pore distribution and relation to adjacent cytoplasmic organelles in cotyledon cells of developing Vicia faba. Planta 141, 121–128
Harris, N. (1979) Endoplasmic reticulum in developing seeds of Vicia faba. A high voltage electron microscope study. Planta 146, 63–69
Hawes, C.R. (1981) Applications of high voltage electron microscopy to botanical ultrastructure. Micron 12, 227–257
Hughes, C.E. (1976) The developing endosperm of Triticum aestivum L.: an ultrastructural and morphometric study. Ph. D. thesis, University of Nottingham
Marty, F. (1973) Sites réactifs à l'iodure de zinc-tétroxyde d'osmium dans les cellules de la racine d'Euphorbia characias L. C. R. Acad. Sci. 277, 1317–1320
Marty, F. (1978) Cytochemical studies on GERL, provacuoles, and vacuoles in root meristematic cells of Euphorbia. Proc. Natl. Acad. Sci. USA 75, 852–856
Marty, F. (1980) High voltage electron microscopy of membrane interactions in wheat. J. Histochem. Cytochem. 28, 1129–1132
Parker, M.L. (1981) Storage protein deposition in developing wheat endosperm. Micron 12, 187–188
Parker, M.L. (1982) Protein accumulation in developing endosperm of a high-protein line of Triticum dicoccoides. Plant Cell Environ. 5 (in press)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Parker, M.L., Hawes, C.R. The Golgi apparatus in developing endosperm of wheat (Triticum aestivum L.). Planta 154, 277–283 (1982). https://doi.org/10.1007/BF00387875
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00387875