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The effect of light pretreatments on phytochrome-mediated induction of anthocyanin and of phenylalanine ammonia-lyase

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Abstract

Induction by light of phenylalanine ammonia-lyase (PAL; EC 4.3.1.5) and of anthocyanin in cotyledons of the mustard (Sinapis alba L.) seedling is strongly affected by a light pretreatment which operates through phytochrome. If PAL or anthocyanin is induced by a light pulse, the effectiveness of phytochrome (Pfr) is strongly increased by a light pretreatment; however, if the increase of the PAL level or synthesis of anthocyanin is elicited by continuous far-red light (operating via phytochrome in the ‘High Irradiance Response’), effectiveness of light is strongly reduced by the same light pretreatment. This reduction of effectiveness is correlated with a decrease of total phytochrome (Ptot) caused by the light pretreatment. It is argued that the observations are compatible only with the ‘open phytochrome-receptor model’ as suggested by Schäfer (J. Mathem. Biol. 2, 41–56, 1975). The peaks of the time courses of the PAL levels under continous far-red light are located at 48 h after sowing and do not depend on the original level of phytochrome. The decrease of the PAL levels beyond 48 h after sowing takes place independently of phytochrome and of the actual level of PAL.

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Abbreviations

Pr :

red absorbing form of phytochrome

Pfr :

far-red absorbing form of phytochrome

Ptot :

total phytochrome (Pr+Pfr)

{ie369-1}:

[Pfr] λ/[Ptot], photoequilibrium of phytochrome at wavelength λ

HIR:

High Irradiance Response

PAL:

phenylalanine ammonialyase (EC 4.3.1.5)

References

  • Drumm, H., Mohr, H.: The dose response curve in phytochrome-mediated anthocyanin synthesis in the mustard seedling. Photochem. Photobiol. 20, 151–157 (1974)

    Google Scholar 

  • Drumm, H., Wildermann, A., Mohr, H.: The ‘high-irradiance response’ in anthocyanin formation as related to the phytochrome level. Photochem. Photobiol. 21, 269–273 (1975)

    PubMed  Google Scholar 

  • Durst, F., Mohr, H.: Phytochrome-mediated induction of enzyme synthesis in mustard seedlings (Sinapis alba L.) Naturwissen-schaften 53, 531–532 (1966)

    Google Scholar 

  • Fox, L.R., Hillman, W.S.: Differences in photoresponse and phytochrome spectrophotometry between etiolated and de-etiolated pea stem tissue. Plant Physiol. 43, 1799–1804 (1968)

    Google Scholar 

  • Frosch, S., Drumm, H., Mohr, H.: Regulation of enzyme levels by phytochrome in mustard cotyledons: multiple mechanisms? Planta 136, 181–186 (1977)

    Google Scholar 

  • Krupp, M.N., Livingston, J.N.: Effects of insulin on insulin-binding components extracted from rat fat cell membranes. Nature 278, 61–62 (1979)

    PubMed  Google Scholar 

  • Lange, H., Shropshire, W., Mohr, H.: An analysis of phytochrome-mediated anthocyanin synthesis. Plant Physiol. 47, 649–655 (1971)

    Google Scholar 

  • Mancinelli, A.L., Rabino, I.: The ‘High Irradiance Responses’ of plant photomorphogenesis. Bot. Rev. 44, 129–177 (1978)

    Google Scholar 

  • McCullough, J.M., Shropshire, W.: Physiological predetermination of germination responses in Arabidopsis thaliana (L.) Heynh. Plant Cell Physiol. 11, 139–148 (1970)

    Google Scholar 

  • Mohr, H.: Untersuchungen zur phytochrominduzierten Photomorphogenese des Senfkeimlings (Sinapis alba L.). Z. Pflanzenphysiol. 54, 63–83 (1966)

    Google Scholar 

  • Mohr, H.: Lectures on Photomorphogenesis. Berlin, Heidelberg, New York: Springer 1972

    Google Scholar 

  • Mohr, H.: The role of phytochrome in controlling enzyme levels in plants. In: MTP International Reviews of Science. Biochemistry Series One. Biochemistry of Cell Differentiation, vol. 9, pp. 37–81. Paul, J., ed. London: Butterworths 1974

    Google Scholar 

  • Mohr, H.: Pattern specification and realization in photomorphogenesis. Bot. Mag. Tokyo Special Issue 1, 199–217 (1978)

    Google Scholar 

  • Mohr, H., Meyer, U., Hartmann, K.M.: Die Beeinflussung der Farnsporen-Keimung [Osmunda cinnamomea (L.) und O. claytoniana (L.)] über das Phytochromsystem und die Photosynthese. Planta 60, 483–496 (1964)

    Google Scholar 

  • Peter, K., Mohr, H.: Control of phenylalanine ammonialyase and ascorbate oxidase in the mustard seedling by light and Hoagland's nutrient solution. Z. Naturforsch. 29c, 222–228 (1974)

    Google Scholar 

  • Pratt, L.H.: Molecular properties of phytochrome. Photochem. Photobiol. 27, 81–105 (1978)

    Google Scholar 

  • Raven, C.W., Shropshire, W.: Photoregulation of logarithmic fluence-response curves for phytochrome control of chlorophyll formation in Pisum sativum L. Photochem. Photobiol. 21, 423–429 (1975)

    Google Scholar 

  • Raven, C.W., Spruit, C.J.P.: Induction of rapid chlorophyll accumulation in dark grown seedlings. III. Transport model for phytochrome action. Acta Bot. Neerl. 22, 135–143 (1973)

    Google Scholar 

  • Schäfer, E.: A new approach to explain the ‘high irradiance response’ of photomorphogenesis on the basis of phytochrome. J. Math. Biol. 2, 41–56 (1975)

    Google Scholar 

  • Schäfer, E., Lassig, T.-U., Schopfer, P.: Photocontrol of phytochrome destruction in grass seedlings. The influence of wavelength and irradiance. Photochem. Photobiol. 22, 193–202 (1975)

    PubMed  Google Scholar 

  • Schäfer, E., Marchal, B., Marmé, D.: In vivo measurements of the phytochrome photostationary state in far-red light. Photochem. Photobiol. 15, 457–464 (1972)

    Google Scholar 

  • Schäfer, E., Schmidt, W., Mohr, H.: Comparative measurements of phytochrome in cotyledons and hypocotyl hook of mustard (Sinapis alba L.). Photochem. Photobiol. 18, 331–334 (1973)

    Google Scholar 

  • Schopfer, P.: Phytochrome control of enzymes. Annu. Rev. Plant Physiol. 28, 223–252 (1977)

    Google Scholar 

  • Schopfer, P., Mohr, H.: Phytochrome-mediated induction of phenyl-alanine ammonialyase in mustard seedlings. Plant Physiol. 49, 8–10 (1972)

    Google Scholar 

  • Smith, H.: Phytochrome and Photomorphogenesis. London: McGraw-Hill 1975

    Google Scholar 

  • Spruit, C.J.P., Mancinelli, A.L.: Phytochrome in cucumber seeds. Planta 88, 303–310 (1969)

    Article  Google Scholar 

  • Steinitz, B.: The appearance of competence for phytochrome-mediated anthocyanin synthesis and some system properties of the phytochrome involved in mediation of anthocyanin synthesis in Sinapis alba L. Dissertation, University of Freiburg 1976

  • Steinitz, B., Drumm, H., Mohr, H.: The appearance of competence for phytochrome-mediated anthocyanin synthesis in the cotyledons of Sinapis alba L. Planta 130, 23–31 (1976)

    Google Scholar 

  • Steinitz, B., Schäfer, E., Drumm, H., Mohr, H.: Correlation between far-red absorbing phytochrome and response in phytochrome-mediated anthocyanin synthesis. Plant, Cell and Environment (in press)

  • Tanada, T.: Phytochrome control of another phytochrome-mediated process. Plant Physiol. 49, 560–562 (1972)

    Google Scholar 

  • Tong, W.F.: Untersuchungen zum Mechanismus der phytochrom-induzierten Akkumulation von Phenylalaninammoniumlyase in den Kotyledonen des Senfkeimlings (Sinapis alba L.). Dissertation, University of Freiburg 1975

  • Tong, W.F., Schopfer, P.: Phytochrome-mediated de novo synthesis of phenylalanine ammonialyase: A new approach using preinduced mustard seedlings. Proc. Nat. Acad. Sci. USA 73, 4017–4021 (1976)

    Google Scholar 

  • Tong, W.F., Schopfer, P.: Absence of Pfr destruction in the modulation of phenylalanine ammonia-lyase synthesis of mustard cotyledons. Plant Physiol. 61, 59–61 (1978)

    Google Scholar 

  • Weidner, M.: Der DNS-Gehalt von Kotyledonen und Hypokotyl des Senfkeimlings (Sinapis alba L.) bei der phytochromgesteuerten Photomorphogenese. Planta 75, 94–98 (1967)

    Google Scholar 

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Author notes

  1. B. Steinitz

    Present address: Department of Floriculture and Ornamental Horticulture, The Volcani Center, P.O.B. 6, Bet Dagan, Israel

Authors and Affiliations

  1. Biologisches Institut II der Universität Freiburg, Schänzlestraße 1, D-7800, Freiburg, Federal Republic of Germany

    H. Mohr, H. Drumm, R. Schmidt & B. Steinitz

Authors
  1. H. Mohr
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  2. H. Drumm
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  3. R. Schmidt
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  4. B. Steinitz
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Mohr, H., Drumm, H., Schmidt, R. et al. The effect of light pretreatments on phytochrome-mediated induction of anthocyanin and of phenylalanine ammonia-lyase. Planta 146, 369–376 (1979). https://doi.org/10.1007/BF00387810

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  • DOI: https://doi.org/10.1007/BF00387810

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