Abstract
During the first 24 h of in vitro incubation of excised potato tuber (Solanum tuberosum L.) discs, the appearance of phenylalanine ammonia-lyase (PAL; EC 3.4.1.5) and the accumulation of chlorogenic acid are both stimulated by infection with Phytophthora infestans (Mont.) de Bary. Whereas in control tissue the level of PAL reached a stable plateau value after 40 h, in infected tissue it subsequently rose again, in one experiment, as the fungal mycelium developed. In the infected but not the control tissue, the level of chlorogenic acid subsequently fell to about to about 20% of its maximum after 50 h. The time courses of increases in cinnamic acid 4-hydroxylase (CA4H; EC 1.14.13.11; 0–60 h) and of caffeic acid acid o-methyltransferase (COMT; EC 2.1.1.42; 0–160 h) are not altered by fungal infection. If the discs are restored to the tuber environment immediately after excision, by placing them inside a “host tuber”, the activity of PAL as well as those of CA4H and COMT remained at the constant low endogenous level for at least 60 h, irrespective of whether the discs had first been inoculated with P. infestans. The increase in PAL may not be an obligatory feature of the P. infestans/potato compatible interaction but dependent on an underlying wound response. The experiments provide further evidence that PAL is the rate limiting step of chlorogenic acid biosynthesis in potato tuber discs.
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Abbreviations
- PAL:
-
phenylalanine ammonia-lyase
- CA4H:
-
cinnamic acid 4-hydroxylase
- COMT:
-
caffeic acid o-methyltransferase
- CGA:
-
chlrogenic acid (5-o-caffeoylquinic acid)
- gfwt:
-
gram fresh weight
References
Clarke, D.D. (1972) The resistance of potato tissue to the hyphal growth of fungal pathogens. Proc. R. Soc. London, Ser. B, 181, 303–317
Clarke, D.D. (1973) The accumulation of scopolin in potato tissue in response to infection. Physiol. Plant Pathol. 3, 347–358
Clarke, D.D., Kassim, M.Y.A. (1977) Resistance factors in potato tuber tissue to hyphal growth of Phytophthora infestans. Physiol. Plant Pathol 11, 267–277
Crosier, W. (1934) Studies in the biology of Phytophthora infestans. (Mont.) de Bary. Mem. Cornell Univ. Agric. Exp. Stn. 155
Dixon, R.A., Fuller, K.W. (1976) Effects of synthetic auxin levels on phaseollin production and phenylalanine ammonia-lyase (PAL) activity in tissue cultures of Phaseolus vulgaris. Physiol. Plant Pathol. 9, 299–312
Doke, N., Garas, N.A., Kuć, J. (1979) Partial characterisation and aspects of the mode of action of a hypersensitivity-inhibiting factor (HIF) isolated from Phytophthora infestans. Physiol. Plant. Pathol. 15, 127–140
Friend, J., Reynolds, S.B., Aveyard, M.A. (1973) Phenylalanine ammonia-lyase, chlorogenic acid and lignin in potato tuber tissue inoculated with Phytophthora infestans. Physiol. Plant Pathol. 3, 495–597
Friend, J., Thornton, J.D. (1974) Caffeic acid-0-methyl transferase, phenolase and peroxidase in potato tuber tissue inoculated with Phytophthora infestans. Phytopathol. Z. 81, 56–64
Garas, N.A., Doke, N., Kuć, J. (1979) Suppression of the hypersensitive reaction in potato tubers by mycelial components from Phytophthora infestans. Physiol. Plant Pathol. 15, 117–126
Gulliver, P.A., Tipton, K.F. (1978) Direct extraction radioassay for catechol-0-methyl-transferase activity. Biochem. Pharmacol. 27, 773–775
Ingram, D.S., Robertson, N.F. (1965) Interaction between Phytophthora infestans and tissue cultures of Solanum tuberosum J. Gen. Microbiol. 40, 431–437
Lamb, C.J. (1977a) Phenylalanine ammonia-lyase and cinnamic acid 4-hydroxylase: Characterisation of the concomitant changes in enzyme activities in illuminated potato tuber discs. Planta 135, 169–175
Lamb, C.J. (1977b) trans-Cinnamic acid as a mediator of the light-stimulated increase in hydroxycinnamoyl-CoA: quinate hydroxycinnamoyl transferase. FEBS. Lett. 75, 37–40
Lamb, C.J. (1979) Regulation of enzyme levels in phenylpropanoid biosynthesis: characterisation of the modulation by light and pathway intermediates. Arch. Biochem. Biophys. 192, 311–317
Lamb, C.J., Merritt, T.K., Butt, V.S. (1979) Synthesis and removal of phenylalanine ammonia-lyase activity in illuminated discs of potato tuber parenchyme. Biochim. Biophys. Acta. 582, 196–212
Lamb, C.J., Rubery, P.H. (1975) A spectrophotometric assay for cinnamic acid 4-hydroxylase activity. Anal. Biochem. 68, 554–561
Lamb, C.J., Rubery, P.H. (1976a) Phenylalanine ammonia-lyase and cinnamic acid 4-hydroxylase: Product repression of the level of enzyme activity in potato tuber discs. Planta 130, 283–290
Lamb, C.J., Rubery, P.H. (1976b) Photocontrol of chlorogenic acid biosynthesis in potato tuber discs. Phytochemistry 15, 665–668
Lamb, C.J., Rubery, P.H. (1976c) Differential effects of cycloheximide on the activity of phenylalanine ammonia-lyase and cinnamic acid 4-hydroxylase in light and dark-incubated potato tuber discs. Plant Sci. Lett. 7, 33–37
Lamb, C.J., Rubery, P.H. (1976d) Inhibition of co-operative enzymes by substrate-analogues: Possible implications for the physiological significance of negative co-operativity illustrated by phenylalanine metabolism in higher plants. J. Theor. Biol. 40, 441–447
Mapson, L.W., Swain, T., Tomalin, A.W. (1963) Influence of variety, cultural conditions and temperature of storage on enzymic browning of potato tubers. J. Sci. Food Agric. 14, 673–684
Massala, R., Legrand, M., Fritig, B. (1980) Effect of α-aminooxyacetate, a competitive inhibitor of phenylalanine ammonia-lyase, on the hypersensitive resistance of tobacco to tobacco mosaic virus. Physiol. Plant Pathol. 16, 213–226
Rhodes, M.J., Wooltorton, L.S.C. (1978) The biosynthesis of phenolic compounds in wounded plant storage tissues. In: Biochemistry of of wounded plant storage tissues, pp. 243–282, Kahl, G., ed. de Gruyter, Berlin New York
Rhodes, M.J.C., Wooltorton, L.S.C., Lourenço, E.J. (1979) Purification and properties of hydroxycinnamoyl CoA quinate hydroxycinnamoyl transferase from potatoes. Phytochemistry 18, 1125–1129
Sharpless, T.K., Wolfenden, R. (1967) A purified nonspecific adenosine deaminase from Taka-Diastase. In: Methods in enzymology, nucleic acids, vol. 12, pt. A pp. 126–131, Grossman, L., Moldave, K., eds. Academic Press, New York
Shimony, C., Friend, J. (1977) The ultrastructure of the interaction between Phytophthora infestans (Mont.) de Bary and tuber discs of potato (Solanum tuberosum L.) cv. King Edward. Physiol. Plant Pathol. 11, 243–249
Smith, B.G. (1980) Phenolic metabolism in healthy and blight-infected excised potato tuber tissue. Ph. D. Thesis, University of Cambridge
Smith, B.G., Rubery, P.H. (1979) Modifications of wound-induced changes in phenylalanine ammonia-lyase activity in potato tuber tissue. Plant Sci. Lett. 15, 29–33
Taylor, A.O., Zucker, M. (1966) Turnover and metabolism of chlorogenic acid in Xanthium leaves and potato tubers. Plant Physiol. 41, 1350–1359
Zucker, M. (1965) Induction of phenylalanine deaminase by light and its relation to chlorogenic acid synthesis in potato tuber tissue. Plant Physiol. 40, 779–788
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Smith, B.G., Rubery, P.H. The effects of infection by Phytophthora infestans on the control of phenylpropanoid metabolism in wounded potato tissue. Planta 151, 535–540 (1981). https://doi.org/10.1007/BF00387431
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DOI: https://doi.org/10.1007/BF00387431