Summary
Etioplasts obtained from the primary leaves of dark-grown bean plants contained cytochromes f, b-559LP and b-563 in a molar ratio of approximately 1.0:2.0:1.5. On illumination of the plants there was a lag of between 10 and 15 h before these cytochromes increased in amount, but after 48 h they had increased from 6- to 10-fold on a per plastid basis. The presence of cytochrome b-559HP in the plastids was first detected after 15 h of illumination, which coincided with the commencement of grana formation and the onset of a number of photosynthetic reactions in the greening leaves. After 48 h of illumination the molar ratio for cytochromes f, b-559HP, b-559LP and b-563 was 1.0:1.2:2.8:2.6.
Agranal chloroplasts formed by the exposure of dark-grown plants to intense light flashes contained high amounts of cytochromes f, b-559LP and b-563 but cytochrome b-559HP could not be detected.
As the light-induced formation of cytochromes f, b-559LP and b-563 was substantially inhibited by D-threo chloramphenicol, but not by the L-threo isomer, it seems likely that their formation was dependent on 70S ribosomes. Both chloramphenicol isomers gave plastids which lacked cytochrome b-559HP.
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References
Bendall, D.S.: Oxidation-reduction potential of cytochromes in chloroplasts from higher plants. Biochem. J. 109, 46P-47P (1968).
Bendall, D. S., Davenport, H. E., Hill, R.: Cytochrome components in chloroplasts of the higher plants In: Methods in enzymology, vol. 23A, p. 327–344 (San Pietro, A., ed.). New York: Academic Press 1971.
Boardman, N. K.: Cytochromes of developing and mutant chloroplasts. In: Comparative biochemistry and biophysics of photosynthesis, p. 206–213 (Shibata, K., Takamiya, A., Jagendorf, A. T., Fuller, R. C., eds.). Tokyo: University of Tokyo Press 1968.
Bradbeer, J. W.: The activities of the photosynthetic carbon cycle enzymes of greening bean leaves. New Phytologist 68, 233–245 (1969).
Bradbeer, J. W.: The synthesis of chloroplast enzymes. In: Biosynthesis and its control in plants, in the press (Milborrow, B. V., ed.). London: Academic Press 1973.
Bradbeer, J. W., Clijsters, H., Glydenholm, A. O., Edge, H. J. W.: Plastid development in primary leaves of Phaseolus vulgaris. The effects of brief flashes of light on dark-grown plants. J. exp. Bot. 21, 525–533 (1970).
Bradbeer, J. W., Ireland, H. M. M., Gyldenholm, A. O., Haslett, B. G., Murray, D. R., Whatley, F. R.: The biosynthesis of the enzymes of the photosynthetic carbon cycle in Phaseolus vulgaris. In: Proceedings of the IInd Internat. Congr. on Photosynthesis Research, vol. 3, in the press (Forti, G., Avron, M., Melandri, A., eds.) The Hague: Dr. W. Junk N.V. 1972.
Ellis, R. J.: Chloroplast ribosomes: stereo-specificity of inhibition by chloramphenicol. Science 163, 477–478 (1969).
Gyldenholm, A. O., Palmer, J. M., Whatley, F. R.: Development of electron transport functions, in greening chloroplasts. In: Energy transduction in respiration and photosynthesis, p. 369–374 (Quagliarcello, E., Papa, S., Rossi, C. S., eds.). Bari: Adriatica Editrice 1971.
Gyldenholm, A. O., Whatley, F. R.: The onset of photophosphorylation in chloroplasts isolated from developing bean leaves. New Phytologist 67, 461–468 (1968).
Ireland, H. M. M., Bradbeer, J. W.: Plastid development in primary leaves of Phaseolus vulgaris. The effects of D-threo and L-threo chloramphenicol on the light-induced formation of enzymes of the photosynthetic carbon pathway. Planta (Berl.) 96, 254–261 (1971).
Irving, A. A.: The beginning of photosynthesis and the development of chlorophyll. Ann. Bot. 24, 805–820 (1910).
Koski, V. M.: Chlorophyll formation in seedlings of Zea mays L. Arch. Biochem. 29, 339–343 (1950).
Mackinney, G.: Absorption of light by chlorophyll solutions. J. biol. Chem. 140, 315–322 (1941).
Oelze-Karow, H., Butler, W. L.: The development of photophosphorylation and photosynthesis in greening bean leaves. Plant Physiol. 48, 621–625 (1971).
Plesnicar, M., Bendall, D. S.: The development of photochemical activities during greening of etiolated barley. In: Proceedings, of the IInd Internat. Congr. on Photosynthesis Research, vol. 3, in the press (Forti, G., Avron, M., Melandri, A., eds.). The Hague: Dr. W. Junk N. V. 1972.
Sironval, C., Bronchart, R., Michel, J. M., Brouers, M., Kuyper, Y.: Structure macromoleculaire et activitiés photochimiques des lamelles plastidiales (essais). Bull. Soc. franç. Physiol. végét. 14, 195–225 (1968).
Wallis, M. E., Bradbeer, J. W.: Plastid development in primary leaves of Phaseolus vulgaris. Free nucleotide concentrations in greening leaves. J. exp. Bot. 21, 1039–1047 (1970).
Wara-Aswapati, O.: Study on the development of photosynthetic activity of greening leaves. M.Sc. Dissertation, University, of London (1970).
Whatley, F. R., Gregory, P., Haslett, B. G., Bradbeer, J. W.: Development of electron transport intermediates in greening chloroplasts. In: Proceedings of the IInd Internat. Congr. on Photosynthesis Research, vol. 3, in the press (Forti, G., Avron, M., Melandri, A., eds.). The Hague: Dr. W. Junk N.V. 1972.
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Gregory, P., Bradbeer, J.W. Plastid development in primary leaves of Phaseolus vulgaris: The light-induced development of the chloroplast cytochromes. Planta 109, 317–326 (1973). https://doi.org/10.1007/BF00387100
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DOI: https://doi.org/10.1007/BF00387100