Summary
Hormone-like substances were isolated from culture media of haploid strains of Saccharomyces cerevisiae. The one excreted by cells of mating type a made cells of the α type expand; the other, excreted by α type cells, made cells of the a type expand. Tentatively we call the former a hormone and the latter α hormone. The cell-expanding action of the a hormone was inhibited by actinomycin D, chloramphenicol and cycloheximide. The a hormone was shown to be heat-stable and dialyzable. Both hormones could be extracted with methylene chloride. The abilities of cells to produce these hormones and to respond to them are under control by the mating-type genes.
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Barksdale, A. W.: The sexual hormones of the fungus Achlya. Ann. N. Y. Acad. Sci. 144, 313–319 (1967).
Levi, J. D.: Mating reaction in yeast. Nature (Lond.) 117, 753–754 (1956).
Machlis, L., W. H. Nutting, M. W. Williams, and H. Rapoport: Production, isolation, and characterization of sirenin. Biochemistry 5, 2147–2152 (1966).
Masuda, Y., and S. Wada: Requirement of RNA for the auxin-induced elongation of oat coleoptile. Physiol. Plantarum (Cph.) 19, 1055–1063 (1966).
McMorris, T. C., and A. W. Barksdale: Isolation of a sex hormone from the water mould Achlya bisexualis. Nature (Lond.) 215, 320–321 (1967).
Mortimer, R. K., and D. C. Hawthorne: Yeast genetics. Ann. Rev. Microbiol. 20, 151–168 (1966).
Noodén, L. D., and K. V. Thimann: Evidence for a requirement for protein synthesis for auxin-induced cell enlargement. Proc. nat. Acad. Sci. (Wash.) 50, 194–200 (1963).
Yanagishima, N., and C. Shimoda: Auxin-induced expansion growth of cells and protoplasts of yeast. Physiol. Plantarum (Cph.) 21, 1122–1128 (1968).
——, H. Kumano, T. Takahashi, and N. Takao: Hormonal regulation of mating reaction in yeast. Proc. XII Internat. Congr. Genetics (Tokyo), vol. 1, p. 65. (1968).
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Yanagishima, N. Sexual hormones in yeast. Planta 87, 110–118 (1969). https://doi.org/10.1007/BF00386970
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DOI: https://doi.org/10.1007/BF00386970