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Induced production of invertase in sugar-beet root by γ-irradiation: Role of RNA

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Summary

Gamma-irradiation dosages between 100 and 200 krad greatly stimulate the development of invertase activity in sugar-beet tissue. However, exposure of tissue to 800 krad virtually eliminates the production of invertase. The production of the enzyme in control and irradiated tissue requires RNA and protein synthesis. Failure of 5-fluorouracil to inhibit the development of invertase implies that the synthesis of ribosomal RNA is not required for enzyme production. A close correlation between irradiation-stimulated methylation of sRNA and enzyme production is noted. We suggest that the synthesis or modification of some RNA required for the translation of “masked invertase RNA” is stimulated by γ-irradiation.

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Authors and Affiliations

  1. Horticulture Department, Purdue University, Lafayette, Indiana

    B. P. Stone & Joe H. Cherry

  2. Department of Biology, Austin Peay State University, 37040, Clarksville, Tennessee, USA

    B. P. Stone

Authors
  1. B. P. Stone
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  2. Joe H. Cherry
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Additional information

This research was supported by a contract (C00-1313-30) from the U.S. Atomic Energy Commission. This is journal paper 4473 of the Purdue University Agriculture Experiment Station.

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Stone, B.P., Cherry, J.H. Induced production of invertase in sugar-beet root by γ-irradiation: Role of RNA. Planta 102, 179–189 (1972). https://doi.org/10.1007/BF00386889

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  • DOI: https://doi.org/10.1007/BF00386889

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