Summary
As found earlier, supply of EDTA was obligatory for both flowering and satisfactory vegetative growth in Wolffia microscopica. It is now shown that the metal affecting growth and flowering is most probably iron. Omission of Fe but not of Cu, Zn, Mn and B from the medium markedly affects vegetative growth. There exists also a strong interaction between EDTA and Fe, one being largely inactive in the absence of the other. When Fe-EDDHA is substituted for Fe-citrate and EDTA in the medium, no great effect is seen in vegetative growth, but flowering takes place even under continuous light. Studies with 59Fe show that, in the medium containing Fe-EDDHA, Fe uptake is stimulated several-fold; this is apparently associated with the flowering condition.
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Abbreviations
- EDTA:
-
ethylenediaminetetraacetic acid
- EDDHA:
-
ethylenediamine-di-o-hydroxyphenylacetic acid
References
Frost, A. E., Freedman, H. H., Westerback, S. J., Martell, A. E.: Chelating tendencies of N, N-ethylenebis-[2-(o-hydroxyphenyl)]-glycine. J. Amer. chem. Soc. 80, 530–536 (1958).
Hillman, W. S.: Photoperiodism, chelating agents, and flowering of Lemna perpusilla and L. gibba in aseptic culture. In: Light and life (W. D. McElroy and B. Glass, eds.), p. 673–686. Baltimore: Johns Hopkins Press 1961 (a).
—: Experimental control of flowering in Lemna. III. A relationship between medium composition and the opposite photoperiodic responses of L. perpusilla 6746 and L. gibba G3. Amer. J. Bot. 48, 413–419 (1961b).
—: The Lemnaceae, or duckweeds. Bot. Rev. 27, 221–287 (1961c).
—: Experimental control of flowering in Lemna. IV. Inhibition of photoperiodic sensitivity by copper. Amer. J. Bot. 49, 892–897 (1962).
Holmes, R. S.: Determination of total copper, zinc, cobalt, and lead in soils and soil solutions. Soil Sci. 59, 77–84 (1945).
Kroll, H., Knell, M., Powers, J., Simonian, J.: A phenolic analogue of ethylenediamine tetraacetic acid. J. Amer. chem. Soc. 79, 2024–2025 (1957).
Maheshwari, S. C., Chauhan, O. S.: In vitro control of flowering in Wolffia microscopia. Nature (Lond.) 198, 99–100 (1963).
—, Seth, P. N.: Induction of flowering in Wolffia microscopica by the iron salt of ethylenediamine-di-o-hydroxyphenylacetic acid (Fe-EDDHA). Z. Pflanzenphysiol. 55, 89–91 (1966).
—, Venkataraman, R.: Induction of flowering in a duckweed, Wolffia microscopica, by a new kinin, zeatin. Planta (Berl.) 70, 304–306 (1966).
Munns, D. N., Johnson, C. M.: Removal of heavy metal and halide contamination from macronutrient salts. Plant Physiol. 65, 978–981 (1960).
Smith, G. F., McCurdy, W. H., Jr., Diehl, H.: The colorimetric determination of iron in raw and treated municipal water supplies by use of 4:7-diphenyl-1:10-phananthroline. Analyst 77, 418–422 (1952).
Smith, H. J., McIlrath, W. J., Bogorad, L.: Some effects of iron deficiency on flowering of Xanthium. Bot. Gaz. 118, 174–179 (1957).
Venkataraman, R., Seth, P. N., Maheshwari, S. C.: Studies on the growth and flowering of a short-day plant, Wolffia microscopica. I. General aspects and induction of flowering by cytokinins. Z. Pflanzenphysiol. (in press).
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Seth, P.N., Venkataraman, R. & Maheshwari, S.C. Studies on the growth and flowering of a short-day plant, Wolffia microscopica . Planta 90, 349–359 (1970). https://doi.org/10.1007/BF00386387
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DOI: https://doi.org/10.1007/BF00386387