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Binding of oxytetracycline to E. coli ribosomes

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Abstract

Binding isotherms of oxytetracycline to E. coli MRE-600 ribosomes were measured by equilibrium dialysis. The results are consistent with the presence of two classes of binding sites for the antibiotic on ribosomes having different reactivities. There is one strong binding site for the antibiotic on the ribosome, while the number of weak binding sites is about 500. The association constant for strong complexes is about 103 times greater than the corresponding value for weak complexes. The strong binding of the antibiotic prevents the template dependent association of aminoacyl-tRNA with ribosomes.

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Authors and Affiliations

  1. Institute of Molecular Biology, Academy of Sciences of the U.S.S.R., 117312, Moscow, U.S.S.R.

    S. A. Streltsov, M. K. Kukhanova, A. A. Krayevsky, I. V. Beljavskaja, L. S. Victorova, G. V. Gursky, A. D. Treboganov & B. P. Gottikh

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  1. S. A. Streltsov
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  2. M. K. Kukhanova
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  3. A. A. Krayevsky
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  4. I. V. Beljavskaja
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  5. L. S. Victorova
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  6. G. V. Gursky
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  7. A. D. Treboganov
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  8. B. P. Gottikh
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All-Union Institute of Antibiotics Research, Moscow 113105.

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Streltsov, S.A., Kukhanova, M.K., Krayevsky, A.A. et al. Binding of oxytetracycline to E. coli ribosomes. Mol Biol Rep 1, 391–396 (1974). https://doi.org/10.1007/BF00385671

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  • DOI: https://doi.org/10.1007/BF00385671

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