Summary
A cytokinin bioassay based on bud formation in 10-cell-long caulonema filaments of Funaria hygrometrica is described. The test has high specificity and sensitivity; is completed in 2 days; exhibits linearity between cytokinin concentration in the medium and bud number; and no buds are formed in the absence of a cytokinin.
References
Bopp, M.: Controll of differentiation in fernallies and bryophytes. Ann. Rev. Plant Physiol. 19, 361–380 (1968).
—, u. W. Diekmann: Die Kinetinwirkung bei Caulonemaregeneration. Planta (Berl.) 74, 86–96 (1967).
Brandes, H.: Der Wirkungsmechanismus des Kinetins bei der Induktion von Knospen am Protonema der Laubmoose. Planta (Berl.) 74, 55–71 (1967).
Jahn, H.: Der Winfluß von Kinetin auf die Anlage der Stämmchen von Funaria hygrometrica. Flora (Jena) 154, 568–588 (1964).
Letham, D. S.: Chemistry and physiology of kinetin-like compounds. Ann. Rev. Plant Physiol. 18, 349–364 (1967).
Miller, C. O., F. Skoog, M. H. Von Saltza, and F. M. Strong: Kinetin, a cell division factor from desoxyribonucleic acid. J. Amer. chem. Soc. 77, 1392 (1955).
Skoog, F., H. Q. Hamzi, A. M. Szweykowska, N. J. Leonard, K. L. Carraway, T. Fujii, J. P. Helgeson, and R. N. Leoppky: Cytokinins: structure/activity relationships. Phytochemistry 6, 1169–1192 (1967).
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Hahn, H., Bopp, M. A cytokinin test with high specificity. Planta 83, 115–118 (1968). https://doi.org/10.1007/BF00385140
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DOI: https://doi.org/10.1007/BF00385140