Abstract
The etiolating, intact mustard (Sinapis alba L.) seedling exhibits a distinct temporal pattern of ethylene production. Light, operating through phytochrome, increases the rate of ethylene production without changing the pattern. Ethylene production of the isolated plant parts (segments), added together, exceed the production of the intact system even if the wound effect is taken into account. There is no significant light effect on ethylene production of the segments. Phytochrome-mediated anthocyanin synthesis in the cotyledons is inhibited by ethylene. The responsiveness towards ethylene of the anthocyanin producing metabolic chain is decreased by phytochrome. As anthocyanin synthesis is only partly inhibited under saturating ethylene concentrations in the atmosphere around the seedlings (100 μl l−1), a twofactor analysis becomes feasible. This analysis leads to the result that phytochrome and ethylene show multiplicative behavior, meaning that phytochrome and ethylene act on the same metabolic sequence (leading to anthocyanin) but independently of each other, and at different sites. Therefore, the hypothesis that ethylene mediates the action of phytochrome in anthocyanin synthesis and photomorphogenesis in general appears to be inapplicable.
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Abbreviations
- Pfr :
-
far-red absorbing form of phytochrome
- Pr :
-
red absorbing form of phytochrome
- Ptot :
-
total phytochrome, i.e. [Pr]+[Pfr]
References
Abeles, F.B.: Ethylene in plant biology. New York: Academic Press 1973
Bühler, B.: Untersuchungen zur Wechselwirkung von Phytochrom und Äthylen bei der Anthocyansynthese des Senfkeimlings (Sinapis alba L.). Doct. dissertation, Univ. of Freiburg, West Germany (1977)
Burg, S.P.: Ethylene in plant growth. Proc. Natl. Acad. Sci. USA 70, 591–597 (1973)
Burg, S.P., Burg, E.A.: Auxin stimulated ethylene formation: its relationship to auxin inhibited growth, root geotropism and other plant processes. In: Biochemistry and physiology of plant growth substances. Wightman, F., Setterfield, G., eds., pp. 1275–1294. Ottawa: Runge Press 1968
Craker, L.E., Abeles, F.B., Shropshire, W.: Light-induced ethylene production in Sorghum. Plant Physiol. 51, 1082–1083 (1973)
Craker, L.E., Standley, L.A., Starbuck, M.J.: Ethylene control of anthocyanin synthesis in Sorghum. Plant Physiol. 48, 349–352 (1971)
Craker, L.E., Wetherbee, P.J.: Ethylene, light and anthocyanin synthesis. Plant Physiol. 51, 436–438 (1973a)
Craker, L.E., Wetherbee, P.J.: Ethylene, carbon dioxide, and anthocyanin synthesis. Plant Physiol. 52, 177–179 (1973b)
DeGreef, J., DeProft, M., DeWinter, F.: Gas chromatographic determination of ethylene in large air volumes at the fractional parts-per-billion level. Anal. Chem. 48, 38–41 (1976)
Drumm, H., Mohr, H.: The dose response curve in phytochromemediated anthocyanin synthesis in the mustard seedling. Photochem. Photobiol. 20, 151–157 (1974)
Drumm, H., Wildermann, A., Mohr, H.: The “high irradiance response” in anthocyanin formation as related to the phytochrome level. Photochem. Photobiol. 21, 269–273 (1975)
Friederich, K.E., Mohr, H.: Adenosine 5′-triphosphate content and energy charge during photomorphogenesis of the mustard seedling, Sinapis alba L. Photochem. Photobiol. 22, 49–53 (1975)
Goeschl, J.D., Pratt, H.K., Bonner, B.A.: An effect of light on the production of ethylene and the growth of the plumular portion of etiolated pea seedlings. Plant Physiol. 42, 1077–1080 (1967)
Goeschl, J.D., Pratt, H.K.: Regulatory roles of ethylene in the etiolated growth habit of Pisum sativum. In: Biochemistry and Physiology of Plant Growth Substances Wightman, F., Setterfield, G., eds., pp. 1229–1242. Ottawa: Runge Press 1968
Goeschl, J.D., Rappaport, L., Pratt, H.K.: Ethylene as a factor regulating the growth of pea epicotyls subjected to physical stress. Plant Physiol. 41, 877–884 (1966)
Janes, H.W., Loercher, L., Frenkel, C.: Effects of red light and ethylene on growth of etiolated lettuce seedlings. Plant Physiol. 57, 420–423 (1976)
Kang, B.G., Burg, S.P.: Involvement of ethylene in phytochrome-mediated carotenoid synthesis. Plant Physiol. 49, 631–633 (1972a)
Kang, B.G., Burg, S.P.: Relation of phytochrome-enhanced geotropic sensitivity to ethylene production. Plant Physiol. 50, 132–135 (1972b)
Kang, B.G., Burg, S.P.: Role of ethylene in phytochrome-induced anthocyanin synthesis. Planta 110, 227–235 (1973)
Kang, B.G., Yocum, C.S., Burg, S.P., Ray, P.M.: Ethylene and carbon dioxide: mediation of hypocotyl hook-opening response. Science 156, 958–959 (1967)
Ku, H.S., Suge, H., Rappaport, L., Pratt, H.K.: Stimulation of rice coleoptile growth by ethylene. Planta 90, 333–339 (1970)
Lange, H., Shropshire, W., Mohr, H.: An analysis of phytochrome-mediated anthocyanin synthesis. Plant Physiol. 47, 649–655 (1971)
Lovell, P.H., Moore, K.G.: A comparative study of cotyledons as assimilatory organs. J. Exp. Bot. 21, 1017–1030 (1970)
Mapson, L.W.: Biogenesis of ethylene. Biol. Rev. 44, 155–187 (1969)
Mayak, S., Dilley, D.R.: Regulation of senescence in carnation (Dianthus caryophyllus). Plant Physiol. 58, 663–665 (1976)
Mohr, H.: Der Einfluß monochromatischer Strahlung auf das Längenwachstums des Hypocotyls und auf die Anthocyanbildung bei Keimlin gen von Sinapis alba L. Planta 49, 389–405 (1957)
Mohr, H.: Untersuchungen zur phytochrominduzierten Photomorphogenese des Senfkeimlings (Sinapis alba L) Z. Pflanzenphysiol 54, 63–83 (1966)
Mohr, H.: Lectures on photomorphogenesis. Berlin-Heidelberg-New York: Springer 1972
Mohr, H.: Lectures on structure and significance of science. Berlin-Heidelberg-New York: Springer 1977
Mohr, H., Appuhn, U.: Die Steuerung des Hypocotylwachstums von Sinapis alba L. durch Licht und Gibberellinsäure. Planta 59, 49–67 (1962)
Negm, F.B., Smith, O.E., Kumamoto, J.: The role of phytochrome in an interaction with ethylene and carbon dioxide in overcoming lettuce seed termodormancy. Plant Physiol. 51, 1089–1094 (1973)
Payer, H.D., Sotriffer, U., Mohr, H.: Die Aufnahme von 14CO2 und die Verteilung des 14C auf freie Aminosäuren und auf Proteinaminosäuren in Hellrot und in Blaulicht. [Objekt: Farnvorkeime von Dryopteris filix-mas (L.) Schott]. Planta 85, 270–283 (1969)
Schäfer, E., Marchal, B., Marmé, D.: In vivo measurements of the phytochrome photostationary state in far-red light. Photochem. Photobiol. 15, 457–464 (1972)
Wareing, P.F., Thompson, A.G.: Rapid effects of red light on hormone levels. In: Light and plant development. Smith, H., ed., pp. 285–294. London: Butterworths 1976
Young, R., Pratt, H., Biale, J.B.: Manometric determinations of low concentrations of ethylene. Anal Chem 24, 551–555 (1952)
Zeroni, M., Galil, J., Ben-Yehoshua, S.: Autoinhibition of ethylene formation in non-ripening stages of the fruit of sycomore fig (Ficus sycomorus L.) Plant Physiol. 57, 647–650 (1976)
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Bühler, B., Drumm, H. & Mohr, H. Investigations on the role of ethylene in phytochrome-mediated photomorphogenesis. Planta 142, 109–117 (1978). https://doi.org/10.1007/BF00385128
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DOI: https://doi.org/10.1007/BF00385128