Abstract
A method for purification of tRNA nucleotidyltransferase from Lupinus luteus L. seeds on a preparative scale is presented. This involved ammonium sulfate fractionation and chromatography on DEAE-cellulose, hydroxylapatite, and QAE-Sephadex A-50. The final enzyme prepartion was apparently homogeneous and a 2000-fold purification was achieved. Data presented suggest the existence of only one form of tRNA nucleotidyltransferase in dry lupin seeds.
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Cudny, H., Pietrzak, M. & Kaczkowski, J. Plant tRNA nucleotidyltransferase. Planta 142, 23–27 (1978). https://doi.org/10.1007/BF00385115
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DOI: https://doi.org/10.1007/BF00385115