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Regulation of adenosine 5′-phosphosulfate sulfotransferase activity by H2S and cyst(e)ine in primary leaves of Phaseolus vulgaris L.

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Abstract

During chloroplast development in the primary leaves of Phaseolus vulgaris, the extractable activity of adenosine 5′-phosphosulfate sulfotransferase increased ten-fold. When chloroplast development took place in air enriched with 3.5 μl H2S·l-1 there was a decrease in adenosine 5′-phosphosulfate sulfotransferase activity. Cyst(e)ine in concentrations up to 1 mM (in the external medium) did not affect the increase in adenosine 5′-phosphosulfate sulfotransferase activity in intact plants. In plants with excised roots, 0.75 mM cyst(e)ine inhibited this increase. In green primary leaves, H2S or cyst(e)ine treatment resulted in a decrease of extractable adenosine 5′-phosphosulfate sulfotransferase activity. In intact plants, this effect of cyst(e)ine was observed at a concentration of 1 mM, and in plants with excised roots, 0.25 mM had a comparable effect.

In developing plants, the extractable activities of O-acetyl-L-serine sulfhydrylase (EC 4.2.99.9) and ribulosebisphosphate carboxylase (EC 4.1.1.39.) were not affected by H2S or cyst(e)ine.

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Abbreviations

APS:

adenosine 5′-phosphosulfate

APSSTase:

adenosine 5′phosphosulfate sulfotransferase

BSA:

bovine serum albumin

DTE:

dithioerythritol

EDTA:

ethylenediaminetetra-acetic acid

OASSase:

O-acetyl-L-serine sulfhydrylase

PAPS:

adenosine 3′-phosphate 5′-phosphosulfate

POPOP:

1,4 Di 2-(5-phenyloxazolyl)-benzene

PPO:

2,5-diphenyloxazol

RubP:

ribulose-bisphosphate

RubPCase:

ribulosebiphosphate carboxylase

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Authors and Affiliations

  1. Pflanzenphysiologisches Institut der Universität Bern, Altenbergrain 21, CH-3013, Bern, Switzerland

    Hans-Rudolf Wyss & Christian Brunold

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  1. Hans-Rudolf Wyss
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  2. Christian Brunold
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Additional information

This is no. 8 in the series “Regulation of Sulfate Assimilation in Plants.” The term “cysteine” is used when it is clear that cystine is not involved; “cyst(e)ine” is used for an undefined mixture of cysteine and cystine. The concentrations are expressed in all cases relative to cysteine

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Wyss, HR., Brunold, C. Regulation of adenosine 5′-phosphosulfate sulfotransferase activity by H2S and cyst(e)ine in primary leaves of Phaseolus vulgaris L.. Planta 147, 37–42 (1979). https://doi.org/10.1007/BF00384588

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  • DOI: https://doi.org/10.1007/BF00384588

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