Abstract
A detailed investigation of the receptive surface of the stigma of a dimorphic taxon, Linum grandiflorum, was carried out using scanning electron microscopy, cytochemistry and polyacrylamide gel electrophoresis. The stigma surfaces of the pin and thrum morphs showed distinct differences. The stigma of the pin morph was of the dry type and the papillae were covered with a uniform cuticle-pellicle layer. The stigma of the thrum morph, on the other hand, resembled the wet type; the cuticle-pellicle layer was disrupted at places and a secretion product was released onto the surface of the stigma. Coomassie blue staining material was present on the surface of only the thrum stigma. Although esterases and acid phosphatases were present on the stigma of both the morphs, their activity was invariably higher on the thrum stigma. Polyacrylamide gel electrophoresis of stigma leachates also showed distinct differences in the protein profiles of the two morphs.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Davis, B.J.: Disc electrophoresis II. Methods and application to human serum proteins. Ann. N.Y. Acad. Sci. 121, 404–427 (1964)
Dickinson, H.G., Lewis, D.: The formation of the tryphine coating the pollen grains of Raphanus, and its properties relating to the self-incompatibility system. Proc. R. Soc. London Ser. B 184, 149–165 (1973)
Dickinson, H.G., Lewis, D.: Changes in the pollen grain wall of Linum grandiflorum following compatible and incompatible intraspecific pollinations. Ann. Bot. 38, 23–29 (1974)
Dulberger, R.: Structural dimorphism of stigmatic papillae in distylous Linum species. Am. J. Bot. 61, 238–243 (1974)
Dulberger, R.: Intermorph structural differences between stigmatic papillae and pollen grains in relation to incompatibility in Plumbaginaceae. Proc. R. Soc. London Ser. B 188, 257–274 (1975a)
Dulberger, R.: S-gene action and the significance of characters in the heterostylous syndrome. Heredity 35, 407–415 (1975b)
Heitz, B., Jean, R., Prensier, G.: Observation de la surface du stigmate et des grains de pollen der Linum austriacum L., hétérostyle. C.R. Hebd. Scéances Acad. Sci. Ser. D. Sci. Nat. 273, 2493–2495 (1971)
Heslop-Harrison, J.: Physiology of the pollen grain surface. Proc. R. Soc. London Ser. B 190, 275–299 (1975)
Heslop-Harrison, J.: A new look at pollination. Rep. E. Malling Res. Stn. for 1975 pp. 141–157 (1976)
Heslop-Harrison, J.: Genetics and physiology of angiosperm incompatibility systems. Proc. R. Soc. London Ser. B 202, 73–92 (1978)
Heslop-Harrison, J., Heslop-Harrison, Y.: Enzymic removal of the proteinaceous pellicle of the stigma papilla prevents pollen tube entry in the Caryophyllaceae. Ann. Bot. 39, 163–165 (1975)
Heslop-Harrison, J., Heslop-Harrison, Y., Barber, J.: The stigma surface in incompatibility responses. Proc. R. Soc. London Ser. B 188, 287–297 (1975)
Heslop-Harrison, Y.: The pollen-stigma interaction: pollen tube penetration in Crocus. Ann. Bot. 41, 913–922 (1977)
Heslop-Harrison, Y., Shivanna, K.R.: The receptive surface of the angiosperm stigma. Ann. Bot. 41, 1233–1258 (1977)
Howlett, B.J., Knox, R.B., Paxton, J.D., Heslop-Harrison, J.: Pollen-wall proteins: physicochemical characterization and role in self-incompatibility in Cosmos bipinnatus. Proc. R. Soc. London Ser. B 188, 167–182 (1975)
Knox, R.B., Heslop-Harrison, J.: Cytochemical localization of enzymes in the walls of the pollen grains. Nature (London) 223, 92–94 (1969)
Knox, R.B., Clarke, A.E., Harrison, S., Smith, P., Marchalonis, J.J.: Cell recognition in plants: determinants of the stigma surface and their pollen interactions. Proc. Natl. Acad. Sci. USA 73, 2788–2792 (1976)
Lewis, D.: The physiology of incompatibility in plants. II. Linum grandiflorum. Ann. Bot. 7, 115–117 (1943)
Lewis, D.: Comparative incompatibility in angiosperms and fungi. Adv. Genet. 6, 235–285 (1954)
Mattsson, O., Knox, R.B., Heslop-Harrison, J., Heslop-Harrison, Y.: Protein pellicle of stigmatic papillae as a probable recognition site in incompatibility reactions. Nature (London) 247, 298–300 (1974)
Nettancourt, D. de: Incompatibility in Angiosperms. Berlin, Heidelberg, New York: Springer 1977
Roberts, I.N., Stead, A.D., Ockendon, D.J., Dickinson, H.G.: A glycoprotein associated with the acquisition of the selfincompatibility system by maturing stigmas of Brassica oleracea. Planta 146, 179–183 (1979)
Scandalios, J.G.: Genetic control of multiple molecular forms of enzymes in plants: a review. Biochem. Genet. 3, 37–79 (1969)
Shivanna, K.R.: Recognition and rejection phenomena during pollen-pistil interaction. Proc. Indian Acad. Sci. B 88, 115–141 (1979)
Weber, K., Osborn, M.: Proteins and sodium dodecyl sulfate: molecular weight determination on polyacrylamide gels and related procedures. In: The Proteins, vol I, pp. 180–225, Neurath, H., Hill, R.L., eds. New York: Academic Press 1975
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ghosh, S., Shivanna, K.R. Pollen-pistil interaction in Linum grandiflorum . Planta 149, 257–261 (1980). https://doi.org/10.1007/BF00384562
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00384562