Summary
Descendants of a gentamicin resistance plasmid first isolated from the Minneapolis Veterans Administration Hospital in 1975 persisted at the hospital until 1983. During this extended period of time several macroevolutionary variants arose and two classes of variants were shown to persist with the initial plasmid. It was possible to construct a cladistic phylogeny on the basis of restriction endonuclease cleavage patterns of the initial and variant plasmids. Additionally, a plasmid polymorphism comprised of members of the plasmid classes was shown to persist at the hospital throughout the endemic period.
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References
Cohen SN, Brevet J, Cabello F, Chang ACY, Chow J, Kapecko DJ, Kretschmer DJ, Nisen P, Timms K (1978) Macro and microevolution of bacterial plasmids. In: Schlessinger D (ed) Microbiology 1978. American Society for Microbiology, Washington, pp 217–220
Datta D, Hughes VM, Nugent ME, Richards H (1979) Plasmids and transposons and their stability and mutability in bacteria isolated during an outbreak of hospital infection. Plasmid 2:182–196
Davis RW, Botstein D, Roth JR (1980) Large Scale isolation of E. coli plasmid DNA. In: A manual for genetic engineering advanced bacterial genetics. Cold Spring Harbor Laboratory, New York, p 116
Gerding DN, Buxton AE, Hughes RA, Cleary PP, Abraczawski J, Stamm WE (1979) Nosocomial multiply resistant Klebsiella pneumoniae: Epidemiology of an outbreak of apparent index case origin. Antimicrob Agents Chemother 15:608–615
John JF, McNeill WF (1981) Characteristics of Serratia marcescens containing a plasmid coding for genetamicin resistance in nosocomial infections. J Infect Dis 143:810–816
Nugent ME, Bone DH, Datta N (1979) A transposon, Tn732, encoding gentamicin/tobramicin resistance. Nature (London) 282:422–423
Nugent ME, Datta N (1980) Transposable gentamicin resistance in IncW plasmids from Hammersmith Hospital. J Gen Microbiol 121:259–262
O'Brien TF, Ross DG, Gazman MA, Medeiros AA, Hedges RW, Botstein D (1980) Dissemination of an antibiotic resistance plasmid in hospital patient flora. Antimicrob Agents Chemother 17:537–543
Portnoy DA, Moseley SL, Falkow S (1981) Characterization of plasmids and plasmid-associated determinants of Yersinia enterocolitica pathogenesis. Infect Immun 31:775–782
Radloff R, Bauer W, Vinograd J (1967) A dye-bouyant-density method for the detection and isolation of closed circular duplex DNA: The closed circular DNA in HeLa cells. Proc Natl Acad Sci USA 57:1514–1521
Rubens CE, McNeill WF, Farrar WE (1979) Evolution of multiple-antibiotic plasmids mediated by transposable plasmid deoxyribonucleic acid sequences. J Bacteriol 140:713–719
Rubens CE, Farrar WE, McGee ZA, Schaeffner W (1981) Evolution of a plasmid mediating resistance to multiple antimicrobial agents during a prolonged epidemic of nosocomial infections. J Infect Dis 143:170–181
Sadowski PL, Peterson BC, Gerding DN, Cleary PP (1979) Physical characterization of 10 R plasmids obtained from an outbreak of nosocomial Klebsiella pneumoniae infections. Antimicrob Agents Chemother 15:616–624
Southern E (1979) Gel electrophoresis of restriction fragments. In: Wu R (ed) Methods in enzymology, vol 68. Academic Press, New York, p 152
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Communicated by G. O'Donovan
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Lee, S.C., Gerding, D.N. & Cleary, P.P. Plasmid macroevolution in a nosocomial environment: Demonstration of a persistent molecular polymorphism and construction of a cladistic phylogeny on the basis of restriction data. Molec. Gen. Genet. 194, 173–178 (1984). https://doi.org/10.1007/BF00383513
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DOI: https://doi.org/10.1007/BF00383513