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Nucleotide sequence of the iron regulatory gene fur

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Summary

The fur gene of Escherichia coli is involved in all iron-regulated transcriptions hitherto studied. The nucleotide sequence of an 868 basepair fragment containing the fur gene was determined. There was only a single longer reading frame. The amino acid sequence derived from the nucleotide sequence comprised 148 amino acids that together made a polypeptide of 16,795 daltons. The amino acid sequence was confirmed by determination of the amino acid composition, the carboxy-terminal lysine residue and the internal Lys-Lys and Lys-Arg sequences of the isolated Fur protein. The nucleotide sequence contains typical initiation and termination sites for transcription and translation.

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Authors and Affiliations

  1. Lehrstuhl Mikrobiologie II der Universität Tübingen, Auf der Morgenstelle 28, D-7400, Tübingen, Germany

    Sven Schäffer, Klaus Hantke & Volkmar Braun

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  1. Sven Schäffer
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  2. Klaus Hantke
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  3. Volkmar Braun
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Communicated by A. Böck

Dedicated to Prof. Otto Lüderitz on the occasion of his 65th birthday

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Schäffer, S., Hantke, K. & Braun, V. Nucleotide sequence of the iron regulatory gene fur . Molec. Gen. Genet. 200, 110–113 (1985). https://doi.org/10.1007/BF00383321

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  • DOI: https://doi.org/10.1007/BF00383321

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