Abstract
A study was undertaken to evaluate the urine mutagenicity of 63 individuals working in four hospital departments. The exposed group included 38 subjects who were exposed to various cytostatic drugs and/or contaminated material from treated patients. The control group included 25 individuals of the hospital personnel. Urine mutagenicity was monitored by the Ames test using tester strains TA 98 + S9 Mix and TA 102 — S9 Mix. Urine samples were collected before and after the working periods. A total of 29/116 (25%) urine samples were mutagenic for either strain. Among the mutagenic samples, 24/29 were mutagenic for tester strain TA 98 exclusively. No significant correlation could be found between occupational exposure to cytostatic drugs and urine mutagenicity evaluated by the strain TA 98 + S9 Mix. Smoking was the main environmental factor that modulated urine mutagenicity with TA 98. Three subjects in the exposed group had mutagenic urine samples at the end of the working period with strain TA 102 — S9 Mix. This mutagenicity was related to occupational exposure to cisplatin. In the control group, one individual had mutagenic samples before and after the working period. Assessing occupational exposure to cytostatic drugs with strain TA 102 requires additional studies to determine environmental mutagens which can be detected by this strain.
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Anderson RW, Puckett WH, Dana WJ, NGuyen TV, Theiss JC, Matney TS (1982) Risk of handling injectable antineoplastic agents. Am J Hosp Pharm 39:1881–1887
Bennicelli C, Camoirano A, Petruzzelli S, Zanacchi, P. De Flora S (1983) High sensitivity of salmonella TA102 in detecting hexavelent chromium mutagenicity and its reversal by liver and lung preparations. Mutat Res 122:1–5
Bos RP, Leenars AO, Theuws JLG, Henderson PT (1982) Mutagenicity of urine from nurses handling cytostatic drugs, influence of smoking. Int Arch Occup Environ Health 50:359–369
Chasson AL, Grady HJ, Stanley MA (1961) Determination of creatinine by means of automatic chemical analysis. Am J Clin Pathol 35:83–88
De Flora S, Ramel C (1988) Mechanisms of inhibitors of mutagenesis and carcinogenesis classification and overview. Mutat. Res 202:285–306
De Flora S, Camoirano A, Zanacchi P, Bennicelli C (1984) Mutagenicity testing with TA97 and TA102 of 30 DNA-damaging compounds, negative with other salmonella strains. Mutat Res 134:159–165
De Méo M, Miribel V, Botta, A, Laget M, Duménil G (1988) Applicability of the SOS Chromotest to detect urinary mutagenicity caused by smoking. Mutagenesis 3:277–283
De Méo M, Vanelle P, Bernadini E, Laget M, Maldonado G, Jentzer I, Crozet MP, Dumenil G (1992) Evaluation of the mutagenic and genotoxic activities of 48 nitroimidazoles and related imidazole derivatives by the Ames test and the SOS Chromotest Environ Mol Mutagen 19:499–503
De Werk Neal A, Wadden RA, Chiou WL (1983) Exposure of hospital workers to airborne antineoplastic agents. Am J Hosp Pharm 40:597–601
Falck K, Gröhn P, Sorsa M, Vainio H, Heinonen E, Holsti LR (1979) Mutagenicity in urine of nurses handling cytostatic drugs. Lancet 1:1250–1251
Friederich U, Molko F, Hofmann V, Scossa D, Hann D, Würgler FE, Senn HJ (1986) Limitations of the salmonella mammalian microsome assay (AMES test) to determine occupational exposure to cytostatic drugs. Eur J Cancer Clin Oncol 22:567–575
Gibson JF, Baxter PJ, Hedworth-Whitty RB, Gompertz D (1983) Urinary mutagenicity assays: a problem arising from the presence of histidine associated factors in XAD-2 prepared urine concentrates with particular relevance to assays carried out using the bacterial fluctuation test. Carcinogenesis 4:1471–1476
Hirst M, Mills DG, Tse S, Levin L, White DF (1984) Occupational exposure to cyclophosphamide. Lancet 1:186–188
Kado NY, Langley D, Eisenstadt E (1983) A simple modification of the salmonella liquid-incubation assay, increased sensitivity for detecting mutagens in human urine. Mutat Res 121:25–32
Kado NY, Manson C, Eisenstadt E, and Hsiech DPH (1985) The kinetics of mutagen excretion in the urine of cigarette smokers. Mutat Res 157:227–233
Kriebel D, Commoner B, Bollinger, D, Bronsdson A, Gold J, Henry J (1983) Detection of occupational exposure to genotoxic agents with a urinary mutagen assay. Mutat Res 108:67–69
Kolmodin-Hedman B, Hartvig P, Sorsa M, Falck K (1983) Occupational handling of cytostatic drugs. Arch Toxicol 54:25–33
Levin DE, Hollstein M, Christman ME, Schwiers EA, Ames BN (1982) A new salmonella tester strain (TA 102) with A-T base pairs at the site of mutation detects oxidative mutagens. Proc Natl Acad Sci USA 79:7445–7449
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurements with the folin phenol reagent. J Biol Chem 193:265–275
Maron DM, Ames BN (1983) Revised method for the Salmonella mutagenicity test. Mutat Res 113:173–215
McDiarmid MA, Egan T, Furio M, Bonacci M, Watts SR (1986) Sampling for airborne fluorouracil in a hospital drug preparation area. Am J Hosp Pharm 43:1942–1945
Mohtashamipur E, Norpoth K, Lieder F (1985) Isolation of frameshift mutagens from smoker's urine: experiences with 3 methods. Carcinogenesis 6:783–788
Poyen D, De Méo MP, Botta A, Gouvernet J, Duménil G (1988) Handling of cytostatic drugs and urine mutagenesis. Int Arch Occup Environ Health 61:183–188
Sessink PJM, Boer KA, Scheefhals APH, Anzion RBM, Bos RP (1992) Occupational exposure to antineoplastic agents at several departments in a hospital. Int Arch Occup Environ Health 64:105–112
Sorsa M, Hemminki, K, Vainio H (1985) Occupational exposure to anticancer drugs-potential and real hazard. Mutat Res 154:135–149
Staino N, Gallelli JF, Adamson RH, Thorgeirsson SS (1981) Lack of mutagenic activity in urine from hospital pharmacists admixing antitumor drugs. Lancet 1:615–616
van Doorn R, Bos RP, Leijdekkers C.-M, Wagenaars-Zegers MAP, Theuws JLG, Henderson PT (1979) Thioether concentration and mutagenicity of urine from cigarette smokers. Int Arch Occup Environ Health 43:159–166
Venitt S (1988) The use of short-term tests for the detection of genotoxic activity in body fluid and excreta. Mutat Res 205:331–353
Venitt S, Crofton-Sleigh C, Hunt J, Speechley V, Briggs K (1984) Monitoring exposure of nursing and pharmacy personnel to cytostatic drugs: urinary mutation assays and urinary platinum as markers of absorption. Lancet I:74–77
Yamasaki E, Ames BN (1977) Concentration of mutagens from urine by absorption with the non polar resin XAD2: cigarette smokers have mutagenic urine. Proc Natl Acad Sci USA 74:3555–3559
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DeMéo, M.P., Mérono, S., DeBaille, A.D. et al. Monitoring exposure of hospital personnel handling cytostatic drugs and contaminated materials. Int. Arch Occup Environ Heath 66, 363–368 (1995). https://doi.org/10.1007/BF00383141
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DOI: https://doi.org/10.1007/BF00383141