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Additional genes essential for replication of the mini-F plasmid from origin I

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Summary

We isolated a series of Tn5-insertional mutants from the mini-F plasmid, which has a deletion in the origin II region and replicates exclusively from origin I, and found that the mutants that had Tn5 in either the F4 or the F5 gene were defective in their replication. It is concluded that, in addition to the F3 gene on which we have reported previously, both the F4 and the F5 genes are essential for the replication from origin I.

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Authors and Affiliations

  1. Laboratory of Genetics, Department of Biology, Faculty of Science, University of Tokyo, Hongo, 113, Tokyo, Japan

    Kouichi Tanimoto & Tetsuo Iino

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  1. Kouichi Tanimoto
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  2. Tetsuo Iino
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Communicated by M. Takanami

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Tanimoto, K., Iino, T. Additional genes essential for replication of the mini-F plasmid from origin I. Molec. Gen. Genet. 198, 358–359 (1985). https://doi.org/10.1007/BF00383020

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  • DOI: https://doi.org/10.1007/BF00383020

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