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Exposure to urban and rural air pollution: DNA and protein adducts and effect of glutathione-S-transferase genotype on adduct levels

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Abstract

Ambient air in urban areas is polluted by agents suspected of causing cancer in humans. A number of epidemiological studies have revealed an increased cancer risk in urban communities, especially in lung cancer. The relative risk have been estimated to be in the order of 1.5. The objective of this study was to evaluate differences in genotoxic exposure through air pollution in urban and rural areas using DNA and protein adducts as biomarkers. Another objective was to investigate whether the GSTM1 genotype has any effect on adduct level. The analyses included 32P postlabelling of DNA adducts in lymphocytes, enzyme-linked immunosorbent assay for measuring benzo[a]pyrene protein adducts and polymerase chain reaction amplification of the GSTM1 genotype. The study was a cross-sectional study of non-smoking, healthy males from rural and urban Danish areas and from Athens, Greece. All individuals in the study were healthy, non-smoking males. The Danish urban group included 74 university students, the rural group 29 students from agricultural colleges and the Greek group 17 individuals. Adduct levels differed significantly in the three groups with median levels of 0.152 fmol/μg DNA (rural), 0.205 fmol/μg (urban) and 0.285 fmol/μg (Athens). The adduct patterns showed some identical spots, but also specific adducts. Here we report increasing DNA adduct levels comparing residents in rural, small urban and large urban residential areas; we found no influence of GSTM1 genotype on DNA or protein adduct levels in non-smokers exposed to low levels of air pollution.

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References

  • Anttila S, Hirvonen A, Husgafvel Pursiainen K, Karjalainen A, Nurminen T, Vainio H (1994) Combined effect of CYPIA1 inducibility and GSTM1 polymorphism on histological type of lung cancer. Carcinogenesis 15:1133–1135

    Google Scholar 

  • Autrup H (1990) Carcinogen metabolism in cultured human tissues and cells. Carcinogenesis 11:707–712

    PubMed  Google Scholar 

  • Beach AC, Gupta RC (1992) Human biomonitoring and the 32P-postlabeling assay. Carcinogenesis 13:1053–1074

    Google Scholar 

  • Doll R, Peto R (1981) The causes of cancer: quantitative estimates of avoidable risks of cancer in the United States today. J Natl Cancer Inst 66:1191–1308

    Google Scholar 

  • Grinberg-Funes RA, Singh VN, Perera FP, Bell DA, Young TL, Dickey C, Wang LW, Santella RM (1994) Polycyclic aromatic hydrocarbon-DNA adducts in smokers and their relationship to micronutrient levels and the glutathione-S-transferase M1 genotype. Carcinogenesis 15:2449–2454

    PubMed  Google Scholar 

  • Grzybowska E, Hemminki K, Chorazy M (1993a) Seasonal variations in levels of DNA adducts and X-spots in human populations living in different parts of Poland. Environ Health Perspect 99:77–81

    PubMed  Google Scholar 

  • Grzybowska E, Hemminki K, Szeliga J, Chorazy M (1993b) Seasonal variation of aromatic DNA adducts in human lymphocytes and granulocytes. Carcinogenesis 14:2523–2526

    Google Scholar 

  • Gupta RC (1985) Enhanced sensitivity of 32P-postlabelling analysis of aromatic carcinogen: DNA adducts. Cancer Res 45:5656–5662

    PubMed  Google Scholar 

  • Gupta RC, Reddy MV, Randerath K (1982) 32P-postlabelling analysis of non-radioactive aromatic carcinogen-DNA adducts. Carcinogenesis 3:1081–1092

    Google Scholar 

  • Hemminki K, Pershagen G (1994) Cancer risk of air pollution: epidemiological evidence. Environ Health Perspect 102 (Suppl 4):187–192

    Google Scholar 

  • Hemminki K, Grzybowska E, Chorazy M, et al. (1990a) DNA adducts in humans environmentally exposed to aromatic compounds in an industrial area of Poland. Carcinogenesis 11:1229–1231

    Google Scholar 

  • Hemminki K, Grzybowska E, Chorazy M, Twardowska Saucha K, Sroczynski JW, Putman KL, Randerath K, Phillips DH, Hewer A (1990b) Aromatic DNA adducts in white blood cells of coke workers. Int Arch Occup Environ Health 62:467–470

    PubMed  Google Scholar 

  • Hemminki K, Autrup H, Haugen A (1993) Environmental carcinogens: assessment of exposure and effect. In: Tomatis L (ed) Indoor and outdoor air pollution and human cancer. Springer, Berlin Heidelberg New York, pp 89–102

    Google Scholar 

  • Johnston RF, Pickett SC, Barker DL (1990) Autoradiography using storage phosphor technology. Electrophoresis 11:355–360

    Google Scholar 

  • Lewtas J (1988) Genotoxicity of complex mixtures: strategies for the identification and comparative assessment of airborne mutagens and carcinogens from combustion sources. Fundam Appl Toxicol 10:571–589

    Article  Google Scholar 

  • Lewtas J, Lewis C, Zweidinger R, Stevens R, Cupitt L (1992) Sources of genotoxicity and cancer risk in ambient air. Pharmacogenetics 2:288–296

    Google Scholar 

  • Menichini E (1992) Urban air pollution by polycyclic aromatic hydrocarbons: levels and sources of variability. Sci Total Environ 116:109–135

    PubMed  Google Scholar 

  • Mustonen R, Hemminki K (1992) 7-Methylguanine levels in DNA of smokers' and non-smokers' total white blood cells, granulocytes and lymphocytes. Carcinogenesis 13:1951–1955

    PubMed  Google Scholar 

  • Nielsen PS, Autrup H (1994) Diesel exhaust related DNA adducts in garage workers. Clin Chem 40:1456–1458

    Google Scholar 

  • Nielsen T, Jørgensen HE, Grundahl L, Jensen AB, Nielsen PA, Tønnesen J (1995) Traffic PAH and other air pollutants in the center of a large city. Riso National Library, Roskilde

    Google Scholar 

  • Perera FP, Hemminki K, Grzybowska E et al. (1992) Molecular and genetic damage in humans from environmental pollution in Poland. Nature 360:256–258

    PubMed  Google Scholar 

  • Randerath K, Reddy MV, Gupta RC (1981) 32P-labelling test for DNA damage. Proc Natl Acad Sci USA 78:6126–6129

    PubMed  Google Scholar 

  • Randerath K, Reddy MV, Disher RM (1986) Age- and tissue-related DNA modifications in untreated rats: detection by 32P-postlabelling assay and possible significance for spontaneous tumor induction and aging. Carcinogenesis 7:1615–1617

    PubMed  Google Scholar 

  • Randerath K, Putman KL, Osterburg HH, Johnson SA, Morgan DG, Finch CE (1993) Age-dependent increases of DNA adducts (I-compounds) in human and rat brain DNA. Mutat Res 295:11–18

    Article  PubMed  Google Scholar 

  • Reddy MV, Randerath K (1986) Nuclease P1-mediated enhancement of sensitivity of 32P-postlabelling test for structurally diverse DNA adducts. Carcinogenesis 7:1543–1551

    PubMed  Google Scholar 

  • Ryberg D, Hewer A, Phillips DH, Haugen A (1994) Different susceptibility to smoking-induced DNA damage among male and female lung cancer patients. Cancer Res 54:5801–5803

    Google Scholar 

  • Santella RM, Hemminki K, Tang DL, Paik M, Ottman R, Young TL, Savela K, Vodickova L, Dickey C, Whyatt R, Perera FP (1993) Polycyclic aromatic hydrocarbon-DNA adducts in white blood cells and urinary 1-hydroxypyrene in foundry workers. Cancer Epid Biomark Prev 2:59–62

    Google Scholar 

  • Savela K, Hemminki K (1991) DNA adducts in lymphocytes and granulocytes of smokers and nonsmokers detected by the 32P-postlabelling assay. Carcinogenesis 12:503–508

    Google Scholar 

  • Savela K, Hemminki K (1993) Analysis of cigarette-smoke-induced DNA adducts by butanol extraction and nuclease P1-enhanced 32P-postlabelling in human lymphocytes and granulocytes. Environ Health Perspect 101 (Suppl 3):145–150

    Google Scholar 

  • Seidegard J, Pero RW, Miller DG, Beattie EJ (1986) A glutathione transferase in human leukocytes as a marker for the susceptibility to lung cancer. Carcinogenesis 7:751–753

    Google Scholar 

  • Sherson D, Sabro P, Sigsgaard T, Johansen F, Autrup H (1990) Biological monitoring of foundry workers exposed to polycyclic aromatic hydrocarbons. Br J Ind Med 47:448–453

    PubMed  Google Scholar 

  • Shields PG, Bowman ED, Harrington AM, Doan VT, Weston A (1993) Polycyclic aromatic hydrocarbon-DNA adducts in human lung and cancer susceptibility genes. Cancer Res 53:3486–3492

    Google Scholar 

  • Stevens RK, Lewis CW, Dzubay TG, Cupitt LT, Lewtas J (1990) Sources of mutagenic activity in urban fine particles. Toxicol Ind Health 6:81–94

    Google Scholar 

  • Walseth TF, Johnson RA (1979) The enzymatic preparation of [alpha-(32)P]nucleoside triphosphates, cyclic [32P] AMP, and cyclic [32P] GMP. Biochim Biophys Acta 562:11–31

    PubMed  Google Scholar 

  • Wolf CR (1990) Metabolic factors in cancer susceptibility. Cancer Surv 9:437–474

    PubMed  Google Scholar 

  • Zhong S, Howie AF, Ketterer B, Taylor J, Hayes JD, Beckett GJ, Wathen CG, Wolf CR, Spurr NK (1991) Glutathione S-transferase mu locus: use of genotyping and phenotyping assays to assess association with lung cancer susceptibility. Carcinogenesis 12:1533–1537

    Google Scholar 

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Authors and Affiliations

  1. Department of Environmental and Occupational Medicine, Building 180, University of Aarhus, DK-8000, Aarhus C, Denmark

    Per Sabro Nielsen, Henrik Okkels, Torben Sigsgaard & Herman Autrup

  2. National Hellenic Research Foundation, 11635, Athens, Greece

    Soterios Kyrtopoulos

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  1. Per Sabro Nielsen
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  2. Henrik Okkels
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  3. Torben Sigsgaard
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  4. Soterios Kyrtopoulos
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  5. Herman Autrup
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Nielsen, P.S., Okkels, H., Sigsgaard, T. et al. Exposure to urban and rural air pollution: DNA and protein adducts and effect of glutathione-S-transferase genotype on adduct levels. Int. Arch Occup Environ Heath 68, 170–176 (1996). https://doi.org/10.1007/BF00381627

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  • DOI: https://doi.org/10.1007/BF00381627

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