Summary
In vitro cultured corollas of the white flowering mutant W18 of Petunia hybrida synthesize anthocyanins after administration of a bud extract of the white flowering mutant W19. Dihydroquercetin-7-glucoside was identified as the major metabolite from W19, which is converted into anthocyanins in W18. This result supports the genetic evidences with regards to the biosynthetic pathway leading to anthocyanins in this plant. W18 was not able to convert flavanones or flavonols into anthocyanins. This type of experiments may be of value for elucidation of certain aspects of flavonoid biosynthesis.
Similar content being viewed by others
References
Amrhein, N., Zenk, M. H.: Untersuchungen zur Rolle der Phenylalanin-Ammonium-Lyase (PAL) bei der Regulation der Flavonoidsynthese im Buchweizen (Fagopyrum esculentum Moench). Z. Pflanzenphysiol. 64, 145–168 (1971)
Bhatia, I. S., Singh, J., Bajaj, K. L.: A new chromogenic reagent for the detection of phenolic compounds on thin layer plates. J. Chromat. 79, 350–352 (1973)
Birkofer, L., Kaiser, C.: Neue Flavonglycoside aus Petunia hybridia. Z. Naturforsch. 17b, 359–368 (1962)
Engelsma, G.: A comparative investigation of the control of phenylalanine ammonia lyase activity in gherkin and red cabbage seedlings. Acta Bot. Neerl. 19, 403–414 (1970)
Geissman, T. A., Jorgensen, E. C., Lennart Johnson, B.: The chemistry of flower pigmentation in Antirrhinum majus color genotypes I. The flavonoid components of the homozygous P, M, Y color types. Arch. Biochem. Biophys. 49, 368–388 (1954)
Hess, D.: Die Wirkung von Zimtsäuren auf die Anthocyansynthese in isolierten Petalen von Petunia hybrida. Z. Pflanzenphysiol. 56, 12–19 (1967)
Johnston, K. M., Stern, D. J., Waiss, A. C. Jr.: Separation of flavonoid compounds on Sephadex LH-20. J. Chromat. 33, 539–541 (1968)
Klein, A. O., Hagen, C. W. Jr.: Anthocyanin production in detached petals of Impatiens balsamina L. Plant Physiol. 36, 1–9 (1961)
Mabry, T. J., Markham, K. R., Thomas, M. B.: The systematic identification of flavonoids. Berlin-Heidelberg-New York: Springer 1970
Mansell, R. L., Hagen, C. W. Jr.: Metabolism of some exogeneous anthocyanins by detached petals of Impatients balsamina L. Amer. J. Bot. 53, 875–882 (1966)
Patschke, L., Grisebach, H.: Dihydrokaempferol and dihydroquercetin as precursors of kaempferol and quercetin in Pisum sativum. Phytochem. 7, 235–237 (1968)
Reddy, G. M., Coe, E. H. Jr.: Inter-tissue complementation: a simple technique for direct analysis of gene action sequence. Science 138, 149 (1962).
Schmitz, M., Seitz, U.: Hemmung der Anthocyansynthese durch Gibberellinsäure bei Kalluskulturen von Daucus carota. Z. Pflanzenphysiol. 68, 259–265 (1972)
Steiner, A. M.: Der Einfluß der Lichtintensität auf die Akkumulation einzelner Anthocyane in isolierten Petalen von Petunia hybrida. Z. Pflanzenphysiol. 68, 266–271 (1972)
Wagner, R. P., Mitchell, H. K.: Genetics and metabolism. 2nd ed. p. 286. New York: Wiley 1964
Wellman, E., Baron, D.: Durch Phytochrom kontrollierte Enzyme der Flavonoidsynthese in Zellsuspensionskulturen von Petersilie (Petroselinum hortense Hoffm.). Planta (Berl.) 119, 161–164 (1974)
Wiering, H.: Genetics of flower colour in Petunia hybrida Hort. Genen Phaenen 17, 117–134 (1974)
Wong, E., Francis, C. M.: Flavonoids in genotypes of Trifolium subterraneum II.: Mutants of the geraldton variety. Phytochem. 7, 2131–2137 (1968)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kho, K.F.F., Bennink, G.J.H. & Wiering, H. Anthocyanin synthesis in a white flowering mutant of Petunia hybrida by a complementation technique. Planta 127, 271–279 (1975). https://doi.org/10.1007/BF00380724
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00380724