Summary
Up to 80% of the cells of actively growing Chlorella species transformed into large, round, osmotically labile cells with cell walls apparently strongly damaged or completely degraded after a 90 h incubation period with a polysaccharide degrading enzyme mixture.
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Atkinson, A. W., Jr., Gunnning, B. E. S., John, P. C. L.: Sporopollenin in the cell wall of Chlorella and other algae: ultrastructure, chemistry and incorporation of 14C-acetate, studied in synchronous cultures. Planta (Berl.) 107, 1–32 (1972)
Binding, H.: Regeneration und Verschmelzung nackter Laubmoosprotoplasten. Z. Pflanzenphysiol. 55, 305–321 (1966)
Carlson, P. S., Smith, H. H., Dearing, R. D.: Parasexual interspecific plant hybridisation. Proc. nat. Acad. Sci. (Wash.) 69, 2292–2294 (1972)
Cocking, E. C.: Plant cell protoplasts—isolation and development. Ann. Rev. Plant Physiol. 23, 29–50 (1972)
Faegri, K., Iversen, J.: Textbook of pollen analysis. Copenhagen: Munksgaard 1964
Ferenczy, L., Kevei, F., Zsolt, J.: Fusion of fungal protoplasts. Nature (Lond.) 248, 793–794 (1974)
Grambow, H. J., Kao, K. N., Miller, R. A., Gramborg, O. L.: Cell division and plant development from protoplasts of carrot cell suspension cultures. Planta (Berl.) 303, 348–355 (1972)
Koch, W.: Verzeichnis der Sammlung von Algenkulturen am Pflanzenphysiologischen Institut der Universität Göttingen. Arch. Mikrobiol. 47, 402–432 (1964)
Küster, E.: Über Membranbildung an kontrahierten Protoplasten höherer Pflanzen. Z. wiss. Mikr. 56, 63–65 (1939)
Potrykus, J.: Fusion von Protoplasten mit gut sichtbaren Kernen. Naturwissenschaften 58, 328 (1971)
Ruppel, H. G.: Untersuchungen über die Zusammensetzung von Chlorella bei Synchronisation im Licht-Dunkel-Wechsel. Flora 152, 113–138 (1962)
Schaeffer, G. W., Smith, H. H.: Auxin-kinetin interactions in tissue cultures of Nicotinana species and tumor-conditioned hybrids. Plant Physiol. 38, 291–297 (1963)
Soeder, C. J.: Weitere zellmorphologische und physiologische Merkmale von Chlorella-Arten. In: Microalgae and photosynthetic bacteria. (Special issue of Plant and Cell Physiol.) S. 21–34. Tokyo 1963
Takebe, I., Labib, G., Melchers, G.: Regeneration of whole plants from isolated mesophyll protoplasts of tobacco. Naturwissenschaften 58, 318–320 (1971)
Weibull, C.: Bacterial protoplasts. Ann. Rev. Microbiol. 12, 1–26 (1958)
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Dedicated to Professor Dr. W. Menke on his 65th birthday.
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Braun, E., Aach, H.G. Enzymatic degradation of the cell wall of Chlorella . Planta 126, 181–185 (1975). https://doi.org/10.1007/BF00380622
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DOI: https://doi.org/10.1007/BF00380622