Summary
In fern gametophytes (= sporelings) there is a strong correlation between the degree of blue light mediated photomorphogenesis and the protein content of the organism (cf. Mohr, 1963). In a previous paper (Payer et al., 1969) we have shown that blue light specifically increases the rate of protein synthesis in the fern sporelings over the rate which is maintained under red light. — In the present paper blue light mediated protein synthesis has been dealt with further using one representative amino acid, alanine, which was labelled with 14C from 14CO2 under steady state conditions of photosynthetic 14C incorporation under blue or red light.
Synthesis of free alanine is proportional to the rate of photosynthesis (Table 1). For a number of reasons we conclude that alanine is derived directly from primary photosynthetic products. Since the pool size of the thoroughly 14C-labelled pool of free alanine is much less than the actual, pool size of this amino acid, (Table 1), and since the specific activity of the isolated 14C-alanine is much below the value we can expect on the basis of the specific activity of the 14CO2 applied we conclude that there are separate pools of free alanine; “active” (with respect to protein synthesis) and “inactive” pools which do not mingle. Taking into account this possibility of compartmentation of pools of free amino acids we have calculated in the case of 14C-alanine the rate of protein synthesis for two extreme instances (Table 2). A comparison of the theoretical values with the actual data indicates that indeed protein synthesis is fed from “active” pools of amino acids while the “inactive” pools are possibly located in the vacuoles. The total pool of alanine is much larger in red grown than in blue grown sporelings while the active pools seem to have the same size under both conditions. The cells of the red grown sporelings have much larger vacuoles than the cells of the blue grown sporelings.
The rate of protein synthesis is under our conditions 1.8 times higher in blue light than in red light. The rate of turnover of the total protein is 0.29% per hour in the blue and 0.23% in the red light. The absolute turnover of protein is 1.5 times higher in blue light than in red light. It is concluded that the blue light mediated increase of protein synthesis is very real. Blue light must act specifically at the level of polypeptide synthesis.
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Literatur
Aach, H. G., u. U. Heber: Kompartimentierung von Aminosäuren in der Blattzelle. Z. Pflanzenphysiol. 57, 317–328 (1967).
Bassham, J. A., and M. Kirk: Photosynthesis of amino acids. Biochim. biophys. Acta (Amst.) 90, 553–562 (1964).
Davies, J. W., and E. C., Cocking: Protein synthesis in tomato-fruit locule tissue: Incorporation of amino acids into protein by aseptic cell-free systems. Biochem. J. 104, 23–33 (1967a).
—: Protein synthesis in tomato-fruit locule tissue: The sites of synthesis and the pathway of carbon into protein. Planta (Berl.) 76, 285–305 (1967b).
Deimling, A. v., u. H. Mohr: Eine Analyse der durch Blaulicht bewirkten Steigerung der Proteinsynthese bei Farnvorkeimen auf der Ebene der Aminosäuren. Planta (Berl.) 76, 269–284 (1967).
Dougall, D. K.: Biosynthesis of protein amino acids in plant tissue cultures. Plant Physiol. 41, 1411–1415 (1966).
Hall, T. C., and E. C. Cocking: Studies on protein synthesis in tomato cotyledons and leaves. Plant Cell Physiol. 7, 329–356 (1966).
Heber, U.: Protein synthesis in chloroplasts during photosynthesis. Nature 195, 91–92 (1962).
Hellebust, J. A., and R. G. S. Bidwell: Protein turnover in wheat and snapdragon leaves. Canad. J. Bot. 41, 969–983 (1963a).
—: Sources of carbon for the synthesis of protein amino acids in attached photosynthesizing wheat leaves. Canad. J. Bot. 41, 985–994 (1963b).
—: Protein turnover in attached wheat and tobacco leaves. Cand. J. Bot. 42, 1–12 (1964).
Hollemann, J. M., and J. L. Key: Inactive and protein precursor pools of amino acids in the soybean hypocotyle. Plant Physiol. 42, 29–36 (1967).
Kasemir, H., u. H. Mohr: Die Regulation von Chlorophyll- und Proteingehalt in Franvorkeimen durch sichtbare Strahlung. Planta (Berl.) 67, 33–43 (1965).
Kates, J. R., and R. F. Jones: Fluoracetate inhibition of amino acids during photosynthesis of Chlamydomonas reinhardti. Science 143, 145–146 (1964).
McLennan, D. H., H. Beevers, and J. L. Harley: Compartmentation, of acids in plant tissues. Biochem. J. 89, 316–327 (1963).
Mohr, H.: The influence of visible radiation on the germination of archegoniate spores and the growth of the fern protonema. J. Linn. Soc. (Bot.) 58, 287–296 (1963).
Moses, V.: Aufgliederung des Stoffwechsels auf verschiedene Reaktionsräume. In: Die Zelle — Struktur und Funktion (H. Metzner, Hrsg.) Stuttgart: Wissenschaftl. Verlags-GmbH. 1966.
—, and K. K. Lonberg-Holm: The study of metabolic compartmentalization. J. theor. Biol. 10, 336–355 (1966).
Ohlenroth, K., u. H. Mohr: Die Steuerung der Proteinsynthese und der Morphogenese bei Farnvorkeimen durch Licht. Planta (Berl.) 59, 427–441 (1963).
Ongun, A., and C. R. Stocking: Effects of light and dark on the intracellular fate of photosynthetic products. Plant Physiol. 40, 825–831 (1965).
Partheir, B.: Proteinsynthese in grünen Blättern I.: Proteinsynthese in verschiedenen subcellulären Fraktionen nach 14CO2-Photosynthese. Z. Naturforsch. 19b, 235–248 (1964).
Payer, H. D., U. Sotriffer u. H. Mohr: Die Aufnahme von 14CO2 und die Verteilung des 14C auf freie Aminosäuren und auf Proteinaminosäuren bei Farnvorkeimen im hellroten, und blauen Licht. Planta 85, 270–283 (1969).
Racusen, D., and M. Foote: Amino acid turnover and protein synthesis in leaves. Arch. Biochem. Biophys. 51, 68–78 (1960).
—: Protein turnover rate in bean leave disks. Plant Physiol. 37, 640–643 (1962).
Smith, D. C., J. A. Bassham, and M. Kirk: Dynamics of the photosynthesis of carbon compounds II. Amino acid synthesis. Biochim. biophys. Acta (Amst.) 48, 299–313 (1961).
Steward, F. C., R. G. S. Bidwell, and E. W. Yemm: Nitrogen metabolism, respiration and growth of cultured plant tissue. J. exp. Bot., 9, 11–49 (1958).
—, and R. G. S. Bidwell: The free nitrogen compounds in plants considered in relation to metabolism, growth and development. In: Amino acid pools (J. T. Holden, edit.) Amsterdam: Elsevier Publ. 1962.
—: Storage pools and turnover systems in growing and non-growing cells: Experiments with 14C-sucrose, 14C-glutamine, and 14C-asparagine. J. exp. Bot. 17, 726–741 (1966).
Vittorio, P. V., G. Krotkov, and G. B. Reed: Canad. J. Bot. 32, 369 (1954); zit. bei F. C. Steward and R. G. S. Bidwell (1962).
Weigl, J. W., P. M. Warrington, and M. Calvin: The relation, of photosynthesis to respiration. J. Amer. chem. Soc. 73, 5058–5063 (1951).
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Payer, H.D. Untersuchungen zur Kompartimentierung der freien Aminosäure Alanin in den Farnvorkeimen von Dryopteris filix-mas (L.) Schott im Rotlicht und im Blaulicht. Planta 86, 103–115 (1969). https://doi.org/10.1007/BF00379818
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DOI: https://doi.org/10.1007/BF00379818