Summary
Protoplasts of Solanum nigrum and Petunia hybrida have been treated with lethal doses of different inhibitors irreversibly affecting cell metabolism. Survival was expected in fusion products by complementation of the inhibited enzymes. After fusion, three protoplasts survived, formed cell walls and two of them underwent mitoses. One of the multicellular regenerants could be examined cytologically and revealed to be a heterokaryon.
This is a preview of subscription content, log in via an institution to check access.
References
Binding, H.: Fusionsversuche mit isolierten Protoplasten von Petunia hybrida L. Z. Pflanzenphysiol. 72, 422–426 (1974)
Binding, H., Nehls, R.: Regeneration of isolated protoplasts to plants in Solanum duleamara L. Z. Pflanzenphysiol. 85, 279–280 (1977)
Keller, W.A., Melchers, G.: The effect of high pH and calcium on tobacco leaf protoplast fusion. Z. Naturforsch. 28c, 737–741 (1973)
Nehls, R.: Isolation and regeneration of protoplasts from Solanum nigrum L. Plant Sci. Lett. 12, 183–187 (1978)
Wright, W.E.: The isolation of heterokaryons and hybrids by a selective system using irreversible biochemical inhibitors. Exp. Cell Res. 112, 395–407 (1978)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nehls, R. The use of metabolic inhibitors for the selection of fusion products of higher plant protoplasts. Molec. Gen. Genet. 166, 117–118 I (1978). https://doi.org/10.1007/BF00379736
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00379736